Skip to main content
SpringerLink
Log in

Effects of D-amino acids on lipoperoxidation in rat liver and kidney mitochondria

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary.

The effects of the amino acids D-ser, D-asp, and D-ala on lipoperoxidation under conditions of hypertension, alcoholism, and ammonemia in rat liver and kidney mitochondria were studied. Under normal conditions, D-alanine increased in 54% free radicals production in liver mitochondria (p < 0.05). The D-amino acids had no effect on kidney mitochondria. D-ser and D-ala increased lipoperoxidation in spontaneously hypertensive rats (SHR) as compared with their normotensive genetic control Wistar-Kyoto (WKY) rats (p < 0.05). During hypertension and in oxidative stress in the presence of calcium, only D-ala produced 46% and 29% free radicals in liver and kidney mitochondria (p < 0.05), respectively. During chronic alcoholism, D-ser increased lipoperoxidation in 80% in kidney mitochondria (p < 0.05), as compared to control. During ammonemia, D-ser produced 41% free radicals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • M Adachi H Ishii (2002) ArticleTitleRole of mitochondria in alcoholic liver injury Free Radic Biol Med 32 487–491 Occurrence Handle11958949 Occurrence Handle1:CAS:528:DC%2BD38Xhs1yls7o%3D Occurrence Handle10.1016/S0891-5849(02)00740-2

    Article  PubMed  CAS  Google Scholar 

  • L Aguilera-Aguirre JC González-Hernández V Pérez-Vázquez J Ramírez M Clemente-Guerrero R Villalobos-Molina A Saavedra-Molina (2002) ArticleTitleRole of intramitochondrial nitric oxide in rat heart and kidney during hypertension Mitochondrion 1 413–423 Occurrence Handle16120294 Occurrence Handle1:CAS:528:DC%2BD38XktVKntL4%3D Occurrence Handle10.1016/S1567-7249(02)00002-8

    Article  PubMed  CAS  Google Scholar 

  • S Asakura R Konno (1997) ArticleTitleOrigin of D-serine present in urine of mutant mice lacking D-amino acid oxidase activity Amino Acids 12 213–223 Occurrence Handle1:CAS:528:DyaK2sXkvFKgs7w%3D Occurrence Handle10.1007/BF01373002

    Article  CAS  Google Scholar 

  • JA Buege SD Aust (1978) ArticleTitleMicrosomal lipid peroxidation Methods Enzymol 52 302–310 Occurrence Handle672633 Occurrence Handle1:CAS:528:DyaE1MXltVajsw%3D%3D Occurrence Handle10.1016/S0076-6879(78)52032-6

    Article  PubMed  CAS  Google Scholar 

  • E Chávez N García C Zazueta F Correa C Avilés G García EO Balam (2003) ArticleTitleThe composition of the incubation medium influences the sensitivity of mitochondrial permeability transition to cyclosporin A J Bioenerg Biomembr 35 149–156 Occurrence Handle12887013 Occurrence Handle10.1023/A:1023798120239

    Article  PubMed  Google Scholar 

  • A D’Aniello G D’Onofrio M Pischetola G D’Aniello A Vetere L Petrucelli GH Fisher (1993) ArticleTitleBiological role of D-amino acid oxidase and D-aspartate oxidase J Biol Chem 268 26941–26949 Occurrence Handle7903300 Occurrence Handle1:CAS:528:DyaK3sXmsVKnu7o%3D

    PubMed  CAS  Google Scholar 

  • A D’Aniello JM Lee L Petrucelli MM Di Fiore (1998) ArticleTitleRegional decreases of free D-aspartate levels in Alzheimer’s disease Neurosci Lett 250 131–134 Occurrence Handle9697936 Occurrence Handle1:CAS:528:DyaK1cXktFKisL8%3D Occurrence Handle10.1016/S0304-3940(98)00451-0

    Article  PubMed  CAS  Google Scholar 

  • A D’Aniello G Fisher N Migliaccio G Cammisa E D’Aniello P Spinelli (2005) ArticleTitleAmino acids and transaminases activity in ventricular CSF and in brain of normal and Alzheimer patients Neurosci Lett 388 49–53 Occurrence Handle16039064 Occurrence Handle1:CAS:528:DC%2BD2MXptVeltrY%3D Occurrence Handle10.1016/j.neulet.2005.06.030

    Article  PubMed  CAS  Google Scholar 

  • AS Dutta JJ Gormley AS Graham I Briggs JW Growcott A Jamieson (1986) ArticleTitleAnalogues of substance P. Peptides containing D-amino acid residues in various positions of substance P and displaying agonist or receptor selective antagonist effects J Med Chem 29 1163–1171 Occurrence Handle2433442 Occurrence Handle1:CAS:528:DyaL28XktFyltbg%3D Occurrence Handle10.1021/jm00157a008

    Article  PubMed  CAS  Google Scholar 

  • E Fernández D Torrents A Zorzano M Palacin J Chillaron (2005) ArticleTitleIdentification and functional characterization of a novel low affinity aromatic-preferring amino acid transporter (arpAT). One of the few proteins silenced during primate evolution J Biol Chem 280 19364–19372 Occurrence Handle15757906 Occurrence Handle10.1074/jbc.M412516200 Occurrence Handle1:CAS:528:DC%2BD2MXjvFersbk%3D

    Article  PubMed  CAS  Google Scholar 

  • GH Fisher I Arias I Quesada S D’Aniello F Errico MM Di Fiore A D’Aniello (2001) ArticleTitleA fast and sensitive method for measuring picomole levels of total free amino acids in very small amounts of biological tissues Amino Acids 20 163–173 Occurrence Handle11332451 Occurrence Handle1:CAS:528:DC%2BD3MXjvFGmsLs%3D Occurrence Handle10.1007/s007260170057

    Article  PubMed  CAS  Google Scholar 

  • M Friedman (1999) ArticleTitleChemistry, nutrition and microbiology of D-amino acids J Agric Food Chem 47 3457–3479 Occurrence Handle10552672 Occurrence Handle1:CAS:528:DyaK1MXlt12lt7k%3D Occurrence Handle10.1021/jf990080u

    Article  PubMed  CAS  Google Scholar 

  • N Fujii (2005) ArticleTitleD-amino acid in elderly tissues Biol Pharm Bull 28 1585–1589 Occurrence Handle16141520 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWjurzE Occurrence Handle10.1248/bpb.28.1585

    Article  PubMed  CAS  Google Scholar 

  • T Furuchi H Homma (2005) ArticleTitleFree D-aspartate in mammals Biol Pharm Bull 28 1566–1570 Occurrence Handle16141517 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWjurzJ Occurrence Handle10.1248/bpb.28.1566

    Article  PubMed  CAS  Google Scholar 

  • MD Gandhi D Pal AK Mitra (2004) ArticleTitleIdentification and functional characterization of a Na(+)-independent large neutral amino acid transporter (LAT2) on ARPE-19 cells Int J Pharmacol 275 189–200 Occurrence Handle1:CAS:528:DC%2BD2cXivFGktbc%3D Occurrence Handle10.1016/j.ijpharm.2004.01.035

    Article  CAS  Google Scholar 

  • JC González-Hernández L Aguilera-Aguirre V Pérez-Vázquez J Ramírez M Clemente-Guerrero C Cortés-Rojo A Saavedra-Molina (2002) ArticleTitleEffect of D-amino acids on some mitochondrial functions in rat liver mitochondria Amino Acids 24 163–169

    Google Scholar 

  • AG Gornall CJ Bardawill MM David (1949) ArticleTitleDetermination of serum proteins by means of the biuret reaction J Biol Chem 177 751–765 Occurrence Handle18110453 Occurrence Handle1:STN:280:DyaH1M%2Fht1Snuw%3D%3D

    PubMed  CAS  Google Scholar 

  • JM Gutteridge I Nagy L Maidt RA Floyd (1990) ArticleTitleADP-iron as a Fenton reactant: radical reactions detected by spin trapping, hydrogen abstraction, and aromatic hydroxylation Arch Biochem Biophys 277 422–428 Occurrence Handle2155582 Occurrence Handle1:CAS:528:DyaK3cXhtlCrsbo%3D Occurrence Handle10.1016/0003-9861(90)90599-T

    Article  PubMed  CAS  Google Scholar 

  • B Halliwell JM Gutteridge (1999) Free radicals in biology and medicine EditionNumber3 Oxford University Press Oxford

    Google Scholar 

  • K Hamase R Konno A Morikawa K Zaitsu (2005) ArticleTitleSensitive determination of D-amino acids in mammals and the effect of D-amino-acid oxidase activity on their amounts Biol Pharm Bull 28 1578–1584 Occurrence Handle16141519 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWjurzL Occurrence Handle10.1248/bpb.28.1578

    Article  PubMed  CAS  Google Scholar 

  • K Hanada T Hara M Nishijima (2000) ArticleTitleD-serine inhibits serine palmitoyltransferase, the enzyme catalyzing the initial step of sphingolipid biosynthesis FEBS Lett 474 63–65 Occurrence Handle10828452 Occurrence Handle1:CAS:528:DC%2BD3cXjs1Chsb4%3D Occurrence Handle10.1016/S0014-5793(00)01579-9

    Article  PubMed  CAS  Google Scholar 

  • K Imai T Fukushima T Santa H Homma Y Huang M Shirao K Liura (1998) ArticleTitleWhole body autoradiography study on the distribution of [14C]-D-serine administered intravenously to rats Amino Acids 15 351–361 Occurrence Handle9891759 Occurrence Handle1:CAS:528:DyaK1MXls12ltw%3D%3D Occurrence Handle10.1007/BF01320899

    Article  PubMed  CAS  Google Scholar 

  • K Keyer JA Imlay (1996) ArticleTitleSuperoxide accelerates DNA damage elevating free-iron levels Proc Natl Acad Sci USA 93 13635–13640 Occurrence Handle8942986 Occurrence Handle1:CAS:528:DyaK28Xnt1Ggu7s%3D Occurrence Handle10.1073/pnas.93.24.13635

    Article  PubMed  CAS  Google Scholar 

  • E Kosenko N Venediktova Y Kaminsky C Montoliu V Felipo (2003) ArticleTitleSources of oxygen radicals in brain in acute ammonia intoxication in vivo Brain Res 981 193–200 Occurrence Handle12885441 Occurrence Handle1:CAS:528:DC%2BD3sXls1Cnsrk%3D Occurrence Handle10.1016/S0006-8993(03)03035-X

    Article  PubMed  CAS  Google Scholar 

  • B Li Z Zhang (2000) ArticleTitleChemiluminescence flow biosensor for determination of total D-amino acid in serum with immobilized reagents Sens Actuat B 69 70–74 Occurrence Handle10.1016/S0925-4005(00)00405-6

    Article  Google Scholar 

  • G Maracaida V Felipo C Hermenejildo MD Miñana S Grisolia (1992) ArticleTitleAcute ammonemia toxicity is mediated by the NMDA type of glutamate receptors FEBS Lett 296 67–68 Occurrence Handle10.1016/0014-5793(92)80404-5

    Article  Google Scholar 

  • K Nakazono N Watanabe K Matsuno J Sasaki T Sato M Inoue (1991) ArticleTitleDoes superoxide underlie the pathogenesis of hypertension? Proc Natl Acad Sci USA 88 10045–10048 Occurrence Handle1658794 Occurrence Handle1:CAS:528:DyaK38XhvFWisg%3D%3D Occurrence Handle10.1073/pnas.88.22.10045

    Article  PubMed  CAS  Google Scholar 

  • M Navab GM Anantharamaiah S Hama DW Garber M Chaddha G Hough R Lallone AM Fogelman (2002) ArticleTitleOral administration of an Apo A-I mimetic peptide synthesized from D-amino acids dramatically reduces atherosclerosis in mice independent of plasma cholesterol Circulation 105 290–292 Occurrence Handle11804981 Occurrence Handle1:CAS:528:DC%2BD38XhsFSgt7k%3D Occurrence Handle10.1161/hc0302.103711

    Article  PubMed  CAS  Google Scholar 

  • T Nishikawa (2005) ArticleTitleMetabolism and functional roles of endogenous D-serine in mammalian brains Biol Pharm Bull 28 1561–1565 Occurrence Handle16141516 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWjurzI Occurrence Handle10.1248/bpb.28.1561

    Article  PubMed  CAS  Google Scholar 

  • S Oguri M Kumazaki R Kitou H Nonoyama N Tooda (1999) ArticleTitleElucidation of intestinal absorption of D, L-amino acid enantiomers and aging in rats Biochim Biophys Acta 1472 107–114 Occurrence Handle10572931 Occurrence Handle1:CAS:528:DyaK1MXnsFSrsL4%3D

    PubMed  CAS  Google Scholar 

  • SE Ohia OS Awe CA Opere AM LeDay LC Harris NA Sharif (2001) ArticleTitleHypoxia-induced [(3)H]D-aspartate release from isolated bovine retina: Modulation by calcium-channel blockers and glutamatergic agonists and antagonists Curr Eye Res 23 386–392 Occurrence Handle11910529 Occurrence Handle1:STN:280:DC%2BD383jsVGgtw%3D%3D Occurrence Handle10.1076/ceyr.23.5.386.5443

    Article  PubMed  CAS  Google Scholar 

  • V Pérez-Vázquez J Ramírez L Aguilera-Aguirre JC González-Hernández M Clemente-Guerrero S Manzo-Ávalos S Uribe A Saavedra-Molina (2002) ArticleTitleEffect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria Amino Acids 22 405–416 Occurrence Handle12107766 Occurrence Handle10.1007/s007260200024

    Article  PubMed  Google Scholar 

  • AI Pettit RK Wong V Lee S Jennings PA Quinn LL Ng (2002) ArticleTitleIncreased free radical production in hypertension due to increased expression of the NADPH oxidase subunit p22(phox) in lymphoblast cell lines J Hypertension 20 677–683 Occurrence Handle1:CAS:528:DC%2BD38XmvFCnu7Y%3D Occurrence Handle10.1097/00004872-200204000-00025

    Article  CAS  Google Scholar 

  • DP Rajan R Kekuda W Huang LD Devoe FH Leibach PD Prasad V Ganapathy (2000) ArticleTitleCloning and functional characterization of a Na(+)-independent, broad-specific neutral amino acid transporter from mammalian intestine Biochim Biophys Acta 1463 6–14 Occurrence Handle10631289 Occurrence Handle1:CAS:528:DC%2BD3cXjtV2nsg%3D%3D Occurrence Handle10.1016/S0005-2736(99)00224-2

    Article  PubMed  CAS  Google Scholar 

  • MG Rosenfeld EH Leiter (1977) ArticleTitleIsolation and characterization of a mitochondrial D-amino acid oxidase from Neurospora crassa Can J Biochem 55 66–74 Occurrence Handle13919 Occurrence Handle1:CAS:528:DyaE2sXltFelu74%3D Occurrence Handle10.1139/o77-012

    Article  PubMed  CAS  Google Scholar 

  • A Saavedra-Molina E Piña (1991) ArticleTitleStimulation of L-ornithine uptake and L-citrulline and urea biosynthesis by D-arginine Biochem Int 24 349–357 Occurrence Handle1930251 Occurrence Handle1:CAS:528:DyaK3MXlsFGksLo%3D

    PubMed  CAS  Google Scholar 

  • A Saavedra-Molina TM Devlin (1997) ArticleTitleEffect of extra- and intra-mitochondrial calcium on citrulline synthesis Amino Acids 12 293–298 Occurrence Handle1:CAS:528:DyaK2sXkvFKgs7c%3D Occurrence Handle10.1007/BF01373009

    Article  CAS  Google Scholar 

  • R Villalobos-Molina JP Pardo A Saavedra-Molina E Piña (1987) ArticleTitleAccumulation of D-arginine by rat liver mitochondria Biochem Cell Biol 65 1057–1063 Occurrence Handle3454185 Occurrence Handle1:CAS:528:DyaL1cXntVOlsg%3D%3D Occurrence Handle10.1139/o87-138

    Article  PubMed  CAS  Google Scholar 

  • K Voss R Galensa (2000) ArticleTitleDetermination of L- and D-amino acids in foodstuffs by coupling of high-performance liquid chromatography with enzyme reactors Amino Acids 18 339–352 Occurrence Handle10949917 Occurrence Handle1:CAS:528:DC%2BD3cXlvVGht78%3D Occurrence Handle10.1007/PL00010321

    Article  PubMed  CAS  Google Scholar 

  • H Wang H Wolosker JF Morris J Pevsner SH Snyder DJ Selkoe (2002) ArticleTitleNaturally occurring free D-aspartate is a nuclear component of cells in the mammalian hypothalamo-neurohypophyseal system Neuroscience 109 1–4 Occurrence Handle11784695 Occurrence Handle1:CAS:528:DC%2BD38XitVykug%3D%3D Occurrence Handle10.1016/S0306-4522(01)00545-0

    Article  PubMed  CAS  Google Scholar 

  • M Zentella de Piña R Villalobos-Molina A Saavedra-Molina H Riveros-Rosas E Piña (1989) ArticleTitleEffects of moderate chronic ethanol consumption on rat liver mitochondrial functions Alcohol 6 3–7 Occurrence Handle2541737 Occurrence Handle10.1016/0741-8329(89)90066-9

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México

    C. Cortés-Rojo, M. Clemente-Guerrero & A. Saavedra-Molina

Authors
  1. C. Cortés-Rojo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Clemente-Guerrero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Saavedra-Molina
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cortés-Rojo, C., Clemente-Guerrero, M. & Saavedra-Molina, A. Effects of D-amino acids on lipoperoxidation in rat liver and kidney mitochondria. Amino Acids 32, 31–37 (2007). https://doi.org/10.1007/s00726-005-0356-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-005-0356-9

Search

Navigation