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Pea Gβ subunit of G proteins has a role in nitric oxide-induced stomatal closure in response to heat and drought stress

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Abstract

Heterotrimeric G proteins consisting of Gα, Gβ and Gγ subunits act as downstream effectors to regulate multiple functions including abiotic stress tolerance. However, the mechanism of Gβ-mediated heat and drought tolerance is yet to be established. To explore the role of Pisum sativum Gβ subunit (PsGβ) in heat and drought stress, transgenic tobacco plants overexpressing (OEs) PsGβ were raised. Transgenic plants showing ectopic expression of PsGβ performed better under heat and drought stress in comparison with vector control plants. The seed germination, relative water content (RWC) and nitric oxide (NO) induction in the guard cells of transgenic plants were significantly higher in contrast to control plants. PsGβ promoter was isolated and several stress-responsive elements were identified. The change in Gβ expression in response to heat, methyl jasmonate (MeJA), abscisic acid (ABA), drought and salt confirms the presence of heat, low temperature and drought-responsive elements in the PsGβ promoter. Also, heat and drought stress caused the release of NO-induced stomatal closure in the leaves of transgenic tobacco plants OEs PsGβ. The better performance of transgenic plant OEs PsGβ is also attributed to the improved photosynthetic parameters as compared with control plants. These findings suggest a role of PsGβ in the signalling pathway leading to NO-induced stomatal closure during heat and drought stress.

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Acknowledgements

DB is thankful to the UGC for providing research fellowship. DB is grateful to Purnima Kumar and Amit Verma for their help and support during the experiments and writing of this paper.

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Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Deepak Bhardwaj, Afsar Raza Naqvi & Narendra Tuteja

  2. Department of Botany, Central University of Jammu, Jammu and Kashmir, 181143, India

    Deepak Bhardwaj

  3. Department of Botany, University of Delhi, Delhi, India

    Deepak Bhardwaj & Suman Lakhanpaul

  4. Department of Biotechnology, Centurion University of Technology and Management, Bhubaneswar, Odisha, 752050, India

    Ranjan Kumar Sahoo

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  1. Deepak Bhardwaj
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Correspondence to Narendra Tuteja.

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Pisum sativum G-beta subunit of G proteins has been shown here to confer heat and drought stress tolerance in transgenic tobacco by regulating nitric oxide-induced stomatal movements.

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ESM 1

Prediction of distribution of Gβ subunit of G proteins through PlantLoc software- A- Probability percentage of PsGβ; B-Probability Percentage of Arabidopsis AGB1. Chloroplast (CHL), cell wall (CEL), cytoplasm (CYT), endoplasmic reticulum (END), extracellular space (EXC), Golgi apparatus (GOL), mitochondrion (MIT), nucleus (NUC), peroxisome (PER), plasma membrane (PLA), and vacuole (VAC). (DOCX 217 kb)

ESM 2

Primer sequences used in the experiments. (DOCX 11 kb)

ESM 3

Cis-elements identified from the Gβ subunit of various plants using plantCARE software. Y represent YES and (−) represent “Not Found”. (DOCX 14 kb)

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Bhardwaj, D., Sahoo, R.K., Naqvi, A.R. et al. Pea Gβ subunit of G proteins has a role in nitric oxide-induced stomatal closure in response to heat and drought stress. Protoplasma 257, 1639–1654 (2020). https://doi.org/10.1007/s00709-020-01529-6

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