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H2S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress

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Abstract

In current study, we investigated the relationship between hydrogen sulfide (H2S) and nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle in wheat seedlings by water stress. Findings showed that water stress significantly stimulated the production of H2S and NO, the transcript levels and activities of enzymes in AsA-GSH cycle, as well as malondialdehyde (MDA) production and electrolyte leakage, but significantly decreased AsA/DHA and GSH/GSSG. Meanwhile, water stress significantly decreased plant height and dry biomass. Except MDA and electrolyte leakage, above changes induced by water stress were reversed by H2S synthesis inhibitor aminooxyacetic acid (AOA) and NO synthesis inhibitor NG-nitro-L-arginine methyl ester (L-NAME). However, AOA and L-NAME significantly enhanced MDA production and electrolyte leakage, which further decreased plant height and dry biomass of wheat seedlings under water stress. Application of exogenous H2S donor sodium hydrosulfide (NaHS) to AOA-treated plants and application of exogenous NO donor sodium nitroprusside (SNP) to L-NAME-treated plants reversed above effects of AOA and L-NAME, respectively. Application of L-NAME plus water stress significantly decreased NO production induced by water stress. However, application of L-NAME plus water stress had no obvious influence on H2S production induced by water stress, while application of AOA plus water stress significantly reduced the production of H2S and NO induced by water stress. Current findings suggested that H2S acted upstream of NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress.

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Funding

This study was supported by “Key Project of Scientific Research of Higher Education Institution from Education Department of Henan Province (18B180009)”, “Open project of Crop Science Characteristic Discipline of Henan Province”, and “National Science Foundation for Young Scientists of China (Grant No. 31700232)”.

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Author notes

  1. Changjuan Shan and Shang Gao contributed equally to this work.

Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, China

    Changjuan Shan, Baoshi Wang, Haili Sun & Shang Gao

  2. School of Life Sciences, Henan University, Kaifeng, 475004, China

    Hua Li

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  1. Changjuan Shan
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  2. Baoshi Wang
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  3. Haili Sun
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  4. Shang Gao
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  5. Hua Li
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Correspondence to Changjuan Shan or Hua Li.

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Shan, C., Wang, B., Sun, H. et al. H2S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress. Protoplasma 257, 1487–1493 (2020). https://doi.org/10.1007/s00709-020-01510-3

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