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Elevated gibberellin altered morphology, anatomical structure, and transcriptional regulatory networks of hormones in celery leaves

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Abstract

Gibberellins (GAs), as one of the important hormones in regulating the growth and development of higher plants, can significantly promote cell elongation and expansion. Celery is a widely grown leafy vegetable crop with rich nutritional value. However, the effect of gibberellins on celery leaves is unclear. In this paper, the celery variety “Jinnan Shiqin” plants were treated with gibberellic acid (GA3) and paclobutrazol (PBZ, a gibberellin inhibitor). Our results showed that GA3 treatment promoted the growth of celery leaves and caused lignification of celery leaf tissue. In addition, the transcript levels of genes associated with gibberellins, auxin, cytokinins, ethylene, jasmonic acid, abscisic acid, and brassinolide were altered in response to increased or decreased exogenous gibberellins or inhibitor. GA3 may regulate celery growth by interacting with other hormones through crosstalk mechanisms. This study provided a reference for further study of the regulation mechanism of gibberellins metabolism, and exerted effects on understanding the role of gibberellins in the growth and development of celery.

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Abbreviations

CPS:

ent-copalyl diphosphate synthase

GA:

Gibberellin

GA3 :

Gibberellic acid

GA20ox:

GA20-oxidase

GA2ox:

GA2-oxidase

GA3ox:

GA3-oxidase

GGPP:

Geranylgeranyl diphosphate

GID1:

Gibberellin insensitive dwarf1

IPP:

Isopentenyl pyrophosphate

KAO:

ent-kaurenoic acid oxidase

KO:

ent-kaurene oxidase

KS:

ent-kaurene synthase

PBZ:

Paclobutrazol

RT-qPCR:

Quantitative real-time polymerase chain reaction

UV:

Ultraviolet

SHI:

Short internode

SLY1:

Sleepy1

SPY:

Spindly

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Funding

The research was supported by Jiangsu Agricultural Science and Technology Innovation Fund [CX(18)2007], National Natural Science Foundation of China (31272175), and Priority Academic Program Development of Jiangsu Higher Education Institutions Project (PAPD).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Ao-Qi Duan, Kai Feng, Jie-Xia Liu, Feng Que, Zhi-Sheng Xu & Ai-Sheng Xiong

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  1. Ao-Qi Duan
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  2. Kai Feng
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  3. Jie-Xia Liu
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  4. Feng Que
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  6. Ai-Sheng Xiong
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Contributions

Conceived and designed the experiments: ASX AQD. Performed the experiments: AQD KF JXL ZSX. Analyzed the data: AQD KF ASX. Contributed reagents/materials/analysis tools: ASX. Wrote the paper: AQD. Revised the paper: ASX FQ. All authors read and approved the final manuscript.

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Correspondence to Ai-Sheng Xiong.

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Duan, AQ., Feng, K., Liu, JX. et al. Elevated gibberellin altered morphology, anatomical structure, and transcriptional regulatory networks of hormones in celery leaves. Protoplasma 256, 1507–1517 (2019). https://doi.org/10.1007/s00709-019-01396-w

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  • DOI: https://doi.org/10.1007/s00709-019-01396-w

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