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Winter wheat cells subjected to freezing temperature undergo death process with features of programmed cell death

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Abstract

Programmed cell death is a process defined as genetically regulated self-destruction or cell suicide. It can be activated by different internal and external factors, but few studies have investigated whether this process occurs under cold and freezing temperatures. In this study, a freezing treatment (−8 °C for 6 h) induced cell death with features of programmed cell death in suspension cultures of winter wheat (Triticum aestivum L.). This process occurred for 10 days after cold exposure. The death of cells in culture was slow and prolonged, and was accompanied by protoplast shrinkage, DNA fragmentation, and an increase in the level of reactive oxygen species. Other changes observed after the freezing treatment included an increase in the respiration rate, changes in mitochondrial transmembrane potential (∆Ψ m ), and the release of cytochrome c from mitochondria into the cytosol. These findings indicated that mitochondria are involved in the cell death process that occurs after a freezing treatment in cells of winter wheat.

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Acknowledgments

The work was supported by the Ministry of Education and Science of the Russian Federation under the 8266 agreement.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of Sciences, Irkutsk-33, P.O. Box 317, Irkutsk, Russia, 664033

    Irina V. Lyubushkina, Olga I. Grabelnych, Tamara P. Pobezhimova, Aleksey V. Stepanov, Anna V. Fedyaeva, Irina V. Fedoseeva & Victor K. Voinikov

  2. Biological Faculty, Irkutsk State University, Irkutsk, Russia

    Irina V. Lyubushkina & Olga I. Grabelnych

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  1. Irina V. Lyubushkina
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  2. Olga I. Grabelnych
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Correspondence to Olga I. Grabelnych.

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Handling Editor: Heiti Paves

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Lyubushkina, I.V., Grabelnych, O.I., Pobezhimova, T.P. et al. Winter wheat cells subjected to freezing temperature undergo death process with features of programmed cell death. Protoplasma 251, 615–623 (2014). https://doi.org/10.1007/s00709-013-0562-3

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  • DOI: https://doi.org/10.1007/s00709-013-0562-3

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