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Serum albumin and its bilirubin complex as drug-carrier proteins for water-soluble porphyrin: a spectroscopic study

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Abstract

The interactions of bovine serum albumin (BSA) and its bilirubin (BR) macromolecular complex (BR·BSA) with meso-tetrakis-(p-sulfophenyl)porphin (TSPP) have been studied by electronic spectroscopy, and emission and synchronous fluorescence in phosphate buffer at pH 7.4. The parameters of the resulting intermolecular complexes (binding constants, quenching rate constants, etc.) were established. The values of the binding constants for the BSA–TSPP and BR·BSA–TSPP systems were 13 × 104 and 8.0 × 104 dm3 mol−1, respectively. The interaction of TSPP with the proteins is studied by static quenching of protein fluorescence, showing predominantly hydrophobic and electrostatic nature. During the complex-formation process, a bathochromic shift of the TSPP Soret band occurs. The influence of TSPP on conformational changes of the protein molecules was analyzed using synchronous fluorescence spectroscopy. It was found that there is competition of BR with TSPP for binding sites on the protein, resulting in displacement of BR from the complex.

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Acknowledgments

This work was supported by Russian Foundation for Basic Research RFBR Project No. 12-03-31309 mol_a and Grant of the President of the Russian Federation No. SP-6898.2013.4 for young scientists and graduate students engaged in advanced research and development in priority directions of modernization of the Russian economy (2013–2015).

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Authors and Affiliations

  1. Department of Inorganic Chemistry, Ivanovo State University of Chemistry and Technology (ISUCT), Ivanovo, Russian Federation

    Alexey V. Solomonov & Evgeniy V. Rumyantsev

  2. G.A. Krestov Institute of Solution Chemistry of the Russian Academy of Sciences, Ivanovo, Russian Federation

    Elena V. Antina

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  1. Alexey V. Solomonov
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  2. Evgeniy V. Rumyantsev
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  3. Elena V. Antina
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Correspondence to Alexey V. Solomonov.

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Solomonov, A.V., Rumyantsev, E.V. & Antina, E.V. Serum albumin and its bilirubin complex as drug-carrier proteins for water-soluble porphyrin: a spectroscopic study. Monatsh Chem 144, 1743–1749 (2013). https://doi.org/10.1007/s00706-013-1062-z

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  • DOI: https://doi.org/10.1007/s00706-013-1062-z

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