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Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene

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Summary.

Human adenoviruses (Ads) are responsible for a substantial disease burden. Type-specific identification of Ads can help guide therapeutic and disease prevention strategies and aid epidemiological investigations. Immunotyping of Ads by serum neutralization (SN) is laborious and time consuming and depends upon type-specific antisera that are in short supply. A rapid molecular typing assay based on polymerase chain reaction (PCR) amplification and sequencing of Ad hexon gene hyper-variable regions 1–6 (HVR1–6) known to contain type-specific epitopes was evaluated as an alternative to SN. Deduced amino acid sequences of HVR1–6 obtained from all 51 currently recognized Ad prototype strains were well resolved, with the exception of types 15 and 29, which were identical. Of 192 temporally and geographically diverse Ad field isolates sequenced in this study, and 111 previously published sequences, all more closely matched their predicted prototype strains. Ads were also detected and correctly identified directly from 24 clinical specimens positive by culture or antigen detection. PCR and sequencing of HVR1–6 offers a practical alternative to SN for typing most Ads and can be readily adapted for use in laboratories with molecular capabilities.

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References

  • AK Adhikary J Numaga T Kaburaki H Kawashima S Kato M Araie K Miyata H Shimizu F Yagyu E Suzuki H Ushijima (2001) ArticleTitleRapid detection and typing of oculopathogenic Strain of subgenus D adenoviruses by fiber-based PCR and restriction enzyme analysis Invest Opthi Vis Sci 42 2010–2015 Occurrence Handle1:STN:280:DC%2BD3MvksFygug%3D%3D

    CAS  Google Scholar 

  • AK Adhikary T Inada J Numaga E Suzuki H Ushijima U Banik A Mukouyama S Matsuno N Okabe (2004) ArticleTitleCharacterization of hexon and fiber genes of a novel strain of adenovirus involved in epidemic kerato-conjunctivitis J Clin Pathol 57 95–97 Occurrence Handle14693847 Occurrence Handle1:CAS:528:DC%2BD2cXntVeksbw%3D Occurrence Handle10.1136/jcp.57.1.95

    Article  PubMed  CAS  Google Scholar 

  • T Adrian G Wadell JC Hierholzer R Wigand (1986) ArticleTitleDNA restriction analysis of adenovirus prototypes 1 to 41 Arch Virol 91 277–290 Occurrence Handle3022681 Occurrence Handle1:CAS:528:DyaL2sXhtlOqsQ%3D%3D Occurrence Handle10.1007/BF01314287

    Article  PubMed  CAS  Google Scholar 

  • T Ariga Y Shimada K Ohgami (2004) ArticleTitleNew genome type of adenovirus serotype 4 caused nosocomial infections associated with epidemic conjunctivitis in Japan J Clin Microbiol 42 3644–3648 Occurrence Handle15297510 Occurrence Handle1:CAS:528:DC%2BD2cXnt1OgtLs%3D Occurrence Handle10.1128/JCM.42.8.3644-3648.2004

    Article  PubMed  CAS  Google Scholar 

  • FK Athappily R Murali JJ Rux Z Cai RM Burnett (1994) ArticleTitleThe refined crystal structure of hexon, the major coat protein of adenovirus type 2, at 2.9 A resolution J Mol Biol 242 430–455 Occurrence Handle10.1006/jmbi.1994.1593

    Article  Google Scholar 

  • M Benkö B Harrach WC Russell (2000) Adenoviridae. Family Adenoviridae MHV van Regenmortel CM Fauquet DHL Bishop EB Carstens MK Estes SM Lemon J Maniloff MA Mayo DJ McGeoch CR Pringle RB Wickner (Eds) Virus taxonomy SeriesTitleThe Seventh Report of the International Committee on Taxonomy of Viruses Academic Press San Diego, CA 227–238

    Google Scholar 

  • DA Benson I Karsch-Mizrachi DJ Lipman J Ostell BA Rapp DL Wheeler (2002) ArticleTitleGenBank Nucleic Acids Res 30 17–20 Occurrence Handle11752243 Occurrence Handle1:CAS:528:DC%2BD38Xht12ksb0%3D Occurrence Handle10.1093/nar/30.1.17

    Article  PubMed  CAS  Google Scholar 

  • DA Blasiole D Metzgar LT Daum MA Ryan J Wu C Wills CT Le NE Freed CJ Hansen GC Gray KL Russell (2004) ArticleTitleMolecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults J Clin Microbiol 42 1686–1693 Occurrence Handle15071026 Occurrence Handle1:CAS:528:DC%2BD2cXjvFyltLw%3D Occurrence Handle10.1128/JCM.42.4.1686-1693.2004

    Article  PubMed  CAS  Google Scholar 

  • MEG Boursnell V Mautner (1981) ArticleTitleRecombination in adenovirus: crossover sites in intertypic recombinants are located in regions of homology Virology 112 198–209 Occurrence Handle6264680 Occurrence Handle1:CAS:528:DyaL3MXksFOqsLw%3D Occurrence Handle10.1016/0042-6822(81)90625-5

    Article  PubMed  CAS  Google Scholar 

  • L Crawford-Miksza DP Schnurr (1996) ArticleTitleAnalysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues J Virol 70 1836–1844 Occurrence Handle8627708 Occurrence Handle1:CAS:528:DyaK28XpvFaktg%3D%3D

    PubMed  CAS  Google Scholar 

  • L Crawford-Miksza DP Schnurr (1996) ArticleTitleAdenovirus serotype evolution is driven by illegitimate recombination in the hypervariable regions of the hexon protein Virology 224 357–367 Occurrence Handle8874497 Occurrence Handle1:CAS:528:DyaK28Xmt12gs70%3D Occurrence Handle10.1006/viro.1996.0543

    Article  PubMed  CAS  Google Scholar 

  • L Crawford-Miksza RN Nang DP Schnurr (1999) ArticleTitleStrain variation in adenovirus serotypes 4 and 7a causing acute respiratory disease J Clin Microbiol 37 1107–1112 Occurrence Handle10074533 Occurrence Handle1:CAS:528:DyaK1MXitFGkt7Y%3D

    PubMed  CAS  Google Scholar 

  • JC De Jong AG Wermenbol MW Verweij-Uijterwaal KW Slaterus P Wertheim-Van Dillen GJ Van Doornum SH Khoo JC Hierholzer (1996) Adenovirus from AIDS patients, including two new candidate serotypes Ad50 and Ad51 of subgeneus D and B1, respectively Xth International Congress of Virology Jerusalem, Israel 230

    Google Scholar 

  • CA Gaydos JC Gaydos (1995) ArticleTitleAdenovirus vaccines in the U.S. military Mil Med 160 300–304 Occurrence Handle7659229 Occurrence Handle1:STN:280:ByqA1cfitFE%3D

    PubMed  CAS  Google Scholar 

  • WC Gruber DJ Russell C Tibbetts (1993) ArticleTitleFiber gene and genomic origin of human adenovirus type 4 Virology 196 603–611 Occurrence Handle8372435 Occurrence Handle1:CAS:528:DyaK2cXhvV2j Occurrence Handle10.1006/viro.1993.1516

    Article  PubMed  CAS  Google Scholar 

  • JC Hierholzer PG Bingham (1978) ArticleTitleVero microcultures for adenovirus neutralization tests J Clin Microbiol 7 499–506 Occurrence Handle670375 Occurrence Handle1:STN:280:CSeB3MfkvV0%3D

    PubMed  CAS  Google Scholar 

  • JC Hierholzer (1995) Adenoviruses EH Lennette DA Lennette ET Lennette (Eds) Diagnostic procedures for viral, rickettsial, and chlamydial infections EditionNumber7 American Public Health Association Washington DC 169–188

    Google Scholar 

  • JC Hierholzer MT Suggs (1969) ArticleTitleStandardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions Appl Microbiol 18 816–823 Occurrence Handle4984202 Occurrence Handle1:CAS:528:DyaE3cXhtV2msQ%3D%3D

    PubMed  CAS  Google Scholar 

  • JC Hierholzer MT Suggs EC Hall (1969) ArticleTitleStandardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation Appl Microbiol 18 824–833 Occurrence Handle4984203 Occurrence Handle1:CAS:528:DyaE3cXitVSmug%3D%3D

    PubMed  CAS  Google Scholar 

  • JC Hierholzer WC Gamble WR Dowdle (1975) ArticleTitleReference equine antisera to 33 human adenovirus types: homologous and heterologous titers J Clin Microbiol 1 65–74 Occurrence Handle1236869 Occurrence Handle1:STN:280:CSmD38rjtlw%3D

    PubMed  CAS  Google Scholar 

  • JC Hierholzer A Pumarola (1976) ArticleTitleAntigenic characterization of intermediate adenovirus 14-11 strains associated with upper respiratory illness in a military camp Infect Immunol 13 354–359 Occurrence Handle1:STN:280:CSmC28nntFA%3D

    CAS  Google Scholar 

  • JC Hierholzer R Wigand LJ Anderson T Adrian JW Gold (1988) ArticleTitleAdenoviruses from patients with AIDS: a plethora of serotypes and a description of five new s serotypes of subgenus D (types 43–47) J Infect Dis 58 804–813

    Google Scholar 

  • JC Hierholzer YO Stone JR Broderson (1991) ArticleTitleAntigenic relationships among the 47 human adenoviruses determined in reference horse antisera Arch Virol 121 179–197 Occurrence Handle1759904 Occurrence Handle1:CAS:528:DyaK38XhtVeqsLc%3D Occurrence Handle10.1007/BF01316753

    Article  PubMed  CAS  Google Scholar 

  • AH Kidd M Jonsson D Garwicz AE Kajon AG Wermenbol MW Verweij JC De Jong (1996) ArticleTitleRapid subgenus identification of human adenovirus isolates by a general PCR J Clin Microbiol 34 622–627 Occurrence Handle8904426 Occurrence Handle1:CAS:528:DyaK28XhvVant7o%3D

    PubMed  CAS  Google Scholar 

  • L Li H Shimizu LT Doan PG Tung S Okitsu O Nishio E Suzuki JK Seo KS Kim WE Muller H Ushijima (2004) ArticleTitleCharacterization of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea J Clin Microbiol 42 4032–4039 Occurrence Handle15364986 Occurrence Handle1:CAS:528:DC%2BD2cXosVyitbY%3D Occurrence Handle10.1128/JCM.42.9.4032-4039.2004

    Article  PubMed  CAS  Google Scholar 

  • B Lin GJ Vora D Thach (2004) ArticleTitleUse of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses J Clin Microbiol 42 3232–3239 Occurrence Handle15243087 Occurrence Handle1:CAS:528:DC%2BD2cXntFGktb4%3D Occurrence Handle10.1128/JCM.42.7.3232-3239.2004

    Article  PubMed  CAS  Google Scholar 

  • SA Lukashok MS Horwitz (1998) ArticleTitleNew perspectives in adenoviruses Curr Clin Top Infect Dis 18 286–305 Occurrence Handle9779361 Occurrence Handle1:STN:280:DyaK1cvlt1eqtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • F Morfin S Dupuis-Girod S Mundweiler D Falcon D Carrington P Sedlacek M Bierings P Cetkovsky AC Kroes MJ van Tol D Thouvenot (2005) ArticleTitleIn vitro susceptibility of adenovirus to antiviral drugs is species-dependent Antivir Ther 10 225–229 Occurrence Handle15865216 Occurrence Handle1:CAS:528:DC%2BD2MXkvFyru7o%3D

    PubMed  CAS  Google Scholar 

  • P Pring-Åkerblom T Adrian T Kostler (1997) ArticleTitlePCR-based detection and typing of human adenoviruses in clinical samples Res Virol 148 225–231 Occurrence Handle9201813 Occurrence Handle10.1016/S0923-2516(97)83992-1

    Article  PubMed  Google Scholar 

  • P Pring-Åkerblom T Adrian (1993) ArticleTitleThe hexon genes of adenoviruses of subgenus C: comparison of the variable regions Res Virol 144 117–127 Occurrence Handle8511397

    PubMed  Google Scholar 

  • P Pring-Åkerblom T Adrian (1994) ArticleTitleType- and group-specific polymerase chain reaction for adenovirus detection Res Virol 145 25–35 Occurrence Handle8023012

    PubMed  Google Scholar 

  • P Pring-Åkerblom FE Trijssenaar T Adrian (1995) ArticleTitleSequence characterization and comparison of human adenovirus subgenus B and E hexons Virology 212 232–236 Occurrence Handle7676636 Occurrence Handle10.1006/viro.1995.1474

    Article  PubMed  Google Scholar 

  • C Rocholl K Gerber J Daly AT Pavia CL Byington (2004) ArticleTitleAdenoviral infections in children: the impact of rapid diagnosis Pediatrics 113 51–56 Occurrence Handle10.1542/peds.113.1.e51

    Article  Google Scholar 

  • W Saitoh-Inagawa A Oshima K Aoki (1996) ArticleTitleRapid diagnosis of adenovirus conjunctivitis by PCR and restriction fragment length polymorphism analysis J Clin Microbiol 34 2113–2116 Occurrence Handle8862567 Occurrence Handle1:CAS:528:DyaK28Xlslynsbc%3D

    PubMed  CAS  Google Scholar 

  • H Sarantis G Johnson M Brown M Petric R Tellier (2004) ArticleTitleComprehensive detection and serotypeing of human adenoviruses by PCR and sequencing J Clin Microbiol 42 3063–3069 Occurrence Handle10.1128/JCM.42.9.3963-3969.2004

    Article  Google Scholar 

  • D Schnurr ME Dondero (1993) ArticleTitleTwo new candidate adenovirus serotypes Intervirology 36 79–83 Occurrence Handle8294185 Occurrence Handle1:STN:280:ByuC38vit10%3D

    PubMed  CAS  Google Scholar 

  • Y Shimada T Ariga T Yoshitsugu K Aoki S Ohno H Ishiko (2004) ArticleTitleMolecular diagnosis of human adenoviruses D and E by a phylogeny-based classification method using a partial hexon sequence J Clin Microbiol 42 1577–1584 Occurrence Handle15071007 Occurrence Handle1:CAS:528:DC%2BD2cXjvFyltrY%3D Occurrence Handle10.1128/JCM.42.4.1577-1584.2004

    Article  PubMed  CAS  Google Scholar 

  • N Singh-Naz WJ Rodriguez AH Kidd CD Brandt (1988) ArticleTitleMonoclonal antibody enzyme-linked immunosorbent assay for specific identification and typing of subgroup F adenoviruses J Clin Microbiol 26 297–300 Occurrence Handle3343324 Occurrence Handle1:STN:280:BieC3sbntlI%3D

    PubMed  CAS  Google Scholar 

  • MP Simmons H Ochoterena (2000) ArticleTitleGaps as characters in sequence-based phylogenetic analyses Syst Biol 49 369–381 Occurrence Handle12118412 Occurrence Handle1:STN:280:DC%2BD38zntlKjtg%3D%3D Occurrence Handle10.1080/10635159950173889

    Article  PubMed  CAS  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), 4th edn. Sinauer Associates, Sunderland, Massachusetts

  • S Takeuchi N Itoh E Uchio K Aoki S Ohno (1999) ArticleTitleSerotyping of adenoviruses on conjunctival scrapings by PCR and sequence analysis J Clin Microbiol 37 1839–1845 Occurrence Handle10325334 Occurrence Handle1:CAS:528:DyaK1MXjslSrtbk%3D

    PubMed  CAS  Google Scholar 

  • S Takeuchi N Itoh E Uchio K Tanaka N Kitamura H Kanai K Isobe K Aoki S Ohno (1999) ArticleTitleAdenovirus strains of subgenus D associated with nosocomial infection as new etiological agents of epidemic keratoconjunctivitis in Japan J Clin Microbiol 37 3392–3394 Occurrence Handle10488216 Occurrence Handle1:STN:280:DyaK1MvhvFWkug%3D%3D

    PubMed  CAS  Google Scholar 

  • R Wigand (1987) ArticleTitlePitfalls in the identification of adenoviruses J Virol Methods 16 161–169 Occurrence Handle2821047 Occurrence Handle1:STN:280:BieD3cfhvVc%3D Occurrence Handle10.1016/0166-0934(87)90001-2

    Article  PubMed  CAS  Google Scholar 

  • R Wigand N Sehn JC Hierholzer JC de Jong T Adrian (1985) ArticleTitleImmunological and biochemical characterization of human adenoviruses from subgenus B. I. Antigenic relationships Arch Virol 84 63–78 Occurrence Handle3985807 Occurrence Handle1:STN:280:BiqC2szovFU%3D Occurrence Handle10.1007/BF01310554

    Article  PubMed  CAS  Google Scholar 

  • SR Wood IR Sharp EO Caul (1997) ArticleTitleRapid detection and serotyping of adenovirus by direct immuno-fluorescence J Med Virol 51 198–201 Occurrence Handle9139083 Occurrence Handle1:STN:280:ByiB3MfjsFE%3D Occurrence Handle10.1002/(SICI)1096-9071(199703)51:3<198::AID-JMV9>3.0.CO;2-1

    Article  PubMed  CAS  Google Scholar 

  • W Xu MM McDonough DD Erdman (2000) ArticleTitleSpecies-specific identification of human adenoviruses by a multiplex polymerase chain reaction assay J Clin Microbiol 38 4114–4120 Occurrence Handle11060077 Occurrence Handle1:CAS:528:DC%2BD3MXhs1Slurw%3D

    PubMed  CAS  Google Scholar 

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  1. Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, U.S.A.

    X. Lu & D. D. Erdman

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Lu, X., Erdman, D. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol 151, 1587–1602 (2006). https://doi.org/10.1007/s00705-005-0722-7

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