Abstract
A novel “off-on” electrochemiluminescence (ECL) aptasensor based on ECL resonance energy transfer (RET) system from 3,4,9,10-perylenetetracar-boxylic acid/the metal-organic frameworks NH2-MIL-125(Ti) (PTCA/NH2-MIL-125) to Au nanoparticles (Au NPs) was put forward for microcystin-LR (MC-LR) assay. The electrochemiluminescence (ECL) emission spectra of PTCA/NH2-MIL-125 show excellent spectral overlap with the UV-vis absorption spectrum of Au NPs. A linear response ranging from 10 fM (9.95 × 10−6 μg L−1) to 0.1 μM (99.5 μg L−1) was obtained, and the detection limit was 3.6 fM (3.58 × 10−6 μg L−1) under optimal conditions at a potential of − 1.6 V (S/N = 3). Besides, the prepared ECL-RET MC-LR aptasensor exhibited good selectivity and stability.
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Funding
This work was financially supported by the Natural Science Foundation of Jiangsu Province (BK20190928), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (18KJB150001, 19KJB150003), the National Natural Science Foundation of China (51874050, Nos. 51874050 and 21904014) and the Foundation of Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology (BM2012110). This work was also supported in part by Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX20_0946).
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Li, J., Jiang, D., Shan, X. et al. An “off-on” electrochemiluminescence aptasensor for microcystin-LR assay based on the resonance energy transfer from PTCA/NH2-MIL-125(Ti) to gold nanoparticles. Microchim Acta 187, 474 (2020). https://doi.org/10.1007/s00604-020-04453-x
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DOI: https://doi.org/10.1007/s00604-020-04453-x