Abstract
A composite, reduced graphene oxide (rGO) doped with silver nanoparticles (Ag NPs), was prepared by using binary reductants of sodium citrate and hydrazine hydrate. Carbon quantum dots (CQDs) synthesized by papaya peel combined with silver ions to form a CQDs-loaded silver nanoparticle (AgCQDs) nanocomposite. Polymer nanospheres (PNS) were generated via the infinite coordination polymer of ferrocene dicarboxylic acid and employed as carriers to load AgCQDs. The prepared AgCQDs@PNS–PEI has good biocompatibility and electrical conductivity and can be used as a matrix for the immobilization of a secondary antibody (Ab2). A sandwich-type electrochemiluminescence (ECL) immunosensor using AgCQDs@PNS–PEI nanocomposite as probe has been developed for the detection of human chorionic gonadotropin (HCG). The proposed immunosensor exhibits a linear range from 0.00100 to 500 mIU mL−1 and the detection limit is 0.33 μIU mL−1 (S/N = 3) under optimal conditions. The sensor exhibits excellent selectivity, good reproducibility, and high stability. These features demonstrate that the proposed method has promising potential for clinical protein detection and displays a new strategy to fabricate an immunosensor.
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Acknowledgments
We gratefully acknowledge Guilin Fifth People’s Hospital (Guilin, China) for providing the blood samples.
Funding
This work was financially supported by the National Natural Science Foundation of China (21765004) and by the Guangxi Science Foundation of China (2019GXNSFAA245076). This work also received research fund from the State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) (CMEMR2018-C18).
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Qin, D., Jiang, X., Mo, G. et al. Electrochemiluminescence immunoassay of human chorionic gonadotropin using silver carbon quantum dots and functionalized polymer nanospheres. Microchim Acta 187, 482 (2020). https://doi.org/10.1007/s00604-020-04450-0
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DOI: https://doi.org/10.1007/s00604-020-04450-0