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Advances in nanomaterial based optical biosensing and bioimaging of apoptosis via caspase-3 activity: a review

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Abstract

Caspase-3 plays a vital role in intrinsic and extrinsic pathways of programed cell death and in cell proliferation. Its detection is an important tool for early detection of some cancers and apoptosis-related diseases, and for monitoring the efficacy of pharmaceuticals and of chemo- and radiotherapy of cancers. This review (with 72 references) summarizes nanomaterial based methods for signal amplification in optical methods for the determination of caspase-3 activity. Following an introduction into the field, a first large section covers optical assays, with subsections on luminescent and chemiluminescence, fluorometric (including FRET based), and colorimetric assays. Further section summarize methods for bioimaging of caspase-3. A concluding section covers current challenges and future perspectives.

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Acknowledgements

We gratefully thanks for financial support of this work by stem cell research center, Tabriz University of Medical Sciences, Tabriz, Iran.

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Authors and Affiliations

  1. Stem Cell Research Center (SCRC), Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Balal Khalilzadeh & Hojjatollah Nozad Charoudeh

  2. Department of Nanochemistry and Nanotechnology Center, Urmia University, Urmia, 57154, Iran

    Nasrin Shadjou

  3. Department of Chemistry, Faculty of Sciences, Van Yuzuncu Yil University, 65080, Van, Turkey

    Gulsah Saydan Kanberoglu

  4. Faculty of Physics, Iran University of Science and Technology, Tehran, 16846-13114, Iran

    Hadi Afsharan

  5. Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain

    Miguel de la Guardia

  6. Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, 51666-14711, Iran

    Alireza Ostadrahimi

  7. Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Mohammad-Reza Rashidi

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  1. Balal Khalilzadeh
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Khalilzadeh, B., Shadjou, N., Kanberoglu, G.S. et al. Advances in nanomaterial based optical biosensing and bioimaging of apoptosis via caspase-3 activity: a review. Microchim Acta 185, 434 (2018). https://doi.org/10.1007/s00604-018-2980-6

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