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Amorphous titania modified with boric acid for selective capture of glycoproteins

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Abstract

Amorphous titania was modified with boric acid, and the resulting material was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction and X-ray photoelectron spectrometry. The new material, in contrast to conventional boronate affinity materials containing boronic acid ligands, bears boric acid groups. It is shown to exhibit high specificity for glycoproteins, and this was applied to design a method for solid phase extraction of glycoproteins as shown for ribonuclease B, horse radish peroxidase and ovalbumin. Glycoproteins were captured under slightly alkaline environment and released in acidic solutions. The glycoproteins extracted were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The binding capacities for ribonuclease B, horse radish peroxidase and ovalbumin typically are 9.3, 26.0 and 53.0 mg ∙ g−1, respectively. The method was successfully applied to the selective enrichment of ovalbumin from egg white.

Schematic presentation of the capture of glycoproteins by amorphous titania modified with boric acid.

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Funding

This work has been supported by the Natural Science Foundation of Hubei Province of China (no. 2014CFB179).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Shanxia Jin, Liping Liu & Ping Zhou

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  1. Shanxia Jin
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  2. Liping Liu
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  3. Ping Zhou
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Correspondence to Ping Zhou.

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The authors declare that they have no competing interests.

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Jin, S., Liu, L. & Zhou, P. Amorphous titania modified with boric acid for selective capture of glycoproteins. Microchim Acta 185, 308 (2018). https://doi.org/10.1007/s00604-018-2824-4

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