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Hyaluronic acid functionalized nanostructured sensing interface for voltammetric determination of microRNA in biological media with ultra-high sensitivity and ultra-low fouling

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Abstract

An electrochemical biosensor for the detection of microRNA was prepared via chemical grafting of a Methylene Blue labeled reporter (MB-Rep) duplex onto a nanostructured surface that was obtained by electrodeposition of cobalt oxide and poly(o-phenylenediamine). This is followed by the attachment of hyaluronic acid and gold nanoclusters. In the presence of the target (microRNA), the probe-target duplex and the MB-Rep hairpin are formed. These will displace the labeled reporter from the sensor surface, and this results in a decrease of the amperometric signal for MB at a typical working voltage of −0.28 V (vs. Ag/AgCl). The electrode modified with hyaluronic acid possesses a large electroactive surface area and an excellent antifouling property. This makes it useful for ultrasensitive quantitation of microRNA even in complex biological media. The sensor has a linear response in the 100 f. to 0.1 μM microRNA concentration range, and a 33.3 f. detection limit. It was successfully applied to the determination of microRNA in cancer cells.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (21675093, 21422504,21505081), and the Taishan Scholar Program of Shandong Province of China (ts20110829).

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  1. Wei Wang and Silambarasan Jayachandran contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Wei Wang, Silambarasan Jayachandran, Mengru Li, Shenghao Xu & Xiliang Luo

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  1. Wei Wang
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Correspondence to Xiliang Luo.

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Wang, W., Jayachandran, S., Li, M. et al. Hyaluronic acid functionalized nanostructured sensing interface for voltammetric determination of microRNA in biological media with ultra-high sensitivity and ultra-low fouling. Microchim Acta 185, 156 (2018). https://doi.org/10.1007/s00604-018-2694-9

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