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Electrochemiluminescence DNA biosensor based on the use of gold nanoparticle modified graphite-like carbon nitride

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Abstract

The authors describe an electrochemiluminescent (ECL) DNA biosensor that is based on the use of gold nanoparticles (AuNPs) modified with graphite-like carbon nitride nanosheets (g-C3N4 NSs) and carrying a DNA probe. In parallel, nanoparticles prepared from gold-platinum (Au/Pt) alloy and carbon nanotubes (CNTs) were placed on a glassy carbon electrode (GCE). Once the g-C3N4 NHs hybridize with DNA-modified AuNPs, they exhibit strong and stable cathodic ECL activity. The Au/Pt-CNTs were prepared by electrochemical deposition of Au/Pt on the surface of the CNTs in order to warrant good electrical conductivity. On hybridization of immobilized capture probe (S1), target DNA (S2) and labeled signal probe (S3), a sandwich-type DNA complex is formed that produces a stable ECL emission at a typical applied voltage of −1.18 V and in the presence of peroxodisulfate. Under optimized conditions, the method has a response to target DNA that is linearly related to the logarithm of its concentration in the range between 0.04 f. and 50 pM, with a 0.018 f. detection limit.

Schematic presentation of an electrochemiluminescent DNA biosensor based on two-dimensional graphite-like carbon nitride nanosheets (g-C3N4 NSs) hybridized with gold nanoparticles. Abbreviations: MCH: 6-mercapto-1-hexanol; S1: Capture probe; S2: target DNA. 

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Acknowledgements

This work was financially supported by National Natural Science Foundation of China (51273084, 51473067). Excellent Youth Foundation of Shandong Provincial 264 (ZR2015JL019).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Yanhu Wang, Lei Shen, Shenguang Ge, Jinghua Yu & Mei Yan

  2. Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, China

    Lina Zhang

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  1. Yanhu Wang
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  2. Lina Zhang
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Correspondence to Mei Yan.

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Wang, Y., Zhang, L., Shen, L. et al. Electrochemiluminescence DNA biosensor based on the use of gold nanoparticle modified graphite-like carbon nitride. Microchim Acta 184, 2587–2596 (2017). https://doi.org/10.1007/s00604-017-2234-z

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  • DOI: https://doi.org/10.1007/s00604-017-2234-z

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