Abstract
We describe the use of a nanocomposite consisting of graphene and β-cyclodextrin (β-CD) which was used to modify a glassy carbon electrode (GCE) to serve as a matrix for immobilization of hemoglobin (Hb). The composite was characterized by scanning electron microscopy, UV-vis and FTIR spectroscopy. The modified electrode displays an enhanced and well-defined quasi reversible peaks for the heme protein at a formal potential of −0.284 V (vs. Ag/AgCl). The direct electrochemistry of Hb is strongly enhanced at this modified electrode compared to electrodes not modified with graphene or β-CD. The heterogeneous electron transfer rate constant (Ks) is 3.18 ± 0.7 s−1 which indicates fast electron transfer. The biosensor exhibits excellent electrocatalytic activity towards the reduction of bromate, with a linear amperometric response in the 0.1 to 176.6 μM concentration range at a working voltage of −0.33 V. The sensitivity is 3.39 μA μM−1 cm−2, and the detection limit is 33 nM. The biosensor is fast, selective, well repeatable and reproducible, and therefore represents a viable platform for sensing bromate in aqueous samples.
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Acknowledgments
This project was supported by the National Science Council and the Ministry of Education of Taiwan (Republic of China). The financial supports of this work by the Ministry of Science and Technology (MOST), Taiwan (MOST-104-2410-H-182-015 to BSL and NSC101-2113-M-027-001-MY3 to SMC) are gratefully acknowledged.
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Palanisamy, S., Wang, YT., Chen, SM. et al. Direct electrochemistry of immobilized hemoglobin and sensing of bromate at a glassy carbon electrode modified with graphene and β-cyclodextrin. Microchim Acta 183, 1953–1961 (2016). https://doi.org/10.1007/s00604-016-1811-x
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DOI: https://doi.org/10.1007/s00604-016-1811-x