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Highly sensitive uric acid biosensor based on individual zinc oxide micro/nanowires

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Abstract

We describe the use of individual zinc oxide (ZnO) micro/nanowires in an electrochemical biosensor for uric acid. The wires were synthesized by chemical vapor deposition and possess uniform morphology and high crystallinity as revealed by scanning electron microscopy, X-ray diffraction, and photoluminescence studies. The enzyme uricase was then immobilized on the surface of the ZnO micro/nanowires by physical adsorption, and this was proven by Raman spectroscopy and fluorescence microscopy. The resulting uric acid biosensor undergoes fast electron transfer between the active site of the enzyme and the surface of the electrode. It displays high sensitivity (89.74 μA cm−2 mM−1) and a wide linear analytical range (between 0.1 mM and 0.59 mM concentrations of uric acid). This study also demonstrates the potential of the use of individual ZnO micro/nanowires for the construction of highly sensitive nano-sized biosensors.

Individual ZnO micro/nanowire based electrochemical biosensor was constructed. The biosensor displayed a higher sensitivity of 89.74 μA cm−2 mM−1 for uric acid detection.

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Acknowledgments

This work was supported by the National Major Research Program of China (2013CB932600), the Program of International S&T Cooperation (2012DFA50990), NSFC (51232001, 51172022, 50972011), the Research Fund of Co-construction Program from Beijing Municipal Commission of Education, the Fundamental Research Funds for the Central Universities, Program for Changjiang Scholars and Innovative Research Team in University, and the Beijing novel program (2008B19) and the Program for New Century Excellent Talents (NCET-09-0219).

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Authors and Affiliations

  1. State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, People’s Republic of China

    Yanguang Zhao, Xiaoqin Yan, Zhuo Kang, Pei Lin, Xiaofei Fang, Yang Lei, Siwei Ma & Yue Zhang

  2. Key Laboratory of New Energy Materials and Technologies, University of Science and Technology Beijing, Beijing, 100083, People’s Republic of China

    Yue Zhang

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  1. Yanguang Zhao
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  2. Xiaoqin Yan
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  3. Zhuo Kang
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  4. Pei Lin
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  6. Yang Lei
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  7. Siwei Ma
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  8. Yue Zhang
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Correspondence to Xiaoqin Yan or Yue Zhang.

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Zhao, Y., Yan, X., Kang, Z. et al. Highly sensitive uric acid biosensor based on individual zinc oxide micro/nanowires. Microchim Acta 180, 759–766 (2013). https://doi.org/10.1007/s00604-013-0981-z

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  • DOI: https://doi.org/10.1007/s00604-013-0981-z

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