Abstract
We report on a novel electrochemical biosensor that was fabricated by immobilizing hemoglobin (Hb) onto the surface of a gold electrode modified with a chitosan@Fe3O4 nano-composite. The Fe3O4 nanoparticles were prepared by co-precipitation and have an average size of 25 nm. They were dispersed in chitosan solution to obtain the chitosan@Fe3O4 nano-composite particles with an average diameter of 35 nm as verified by transmission electron microscopy. X-ray diffraction patterns and Fourier transform IR spectroscopy confirmed that the crystallite structure of the Fe3O4 particles in the nano-composite has remained unchanged. At pH 7.0, Hb gives a pair of redox peaks with a potential of about −0.21 V and −0.36 V. The Hb on the film maintained its biological activity and displays good electrocatalytic reduction activity towards hydrogen peroxide. The linear range for the determination of H2O2 is from 2.3 μM to 9.6 mM, with a detection limit at 1.1 μM concentration (at S/N = 3). The apparent Michaelis-Menten constant is 3.7 mM and indicates the high affinity of Hb for H2O2. This biosensor also exhibits good reproducibility and long-term stability. Thus, it is expected to possess potential applications in the development of the third-generation electrochemical biosensors.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Grant numbers: 20875051; 81001263), the Natural Science Foundation of Jiangsu Province (Grant number: BK2011047), the Universities Natural Science Foundation of Jiangsu Province (Grant number: 10KJB150015), the Application Research Item of Nantong City (Grant number: BK2011020),the Social Development Item of Nantong City (Grant number: HS2012017) and the project founded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (Grant number: 12KJD150010).
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Yuan-Hong Wang and Chun-Mei Yu, co-first author. Paper was written by Yuan-Hong Wang
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Wang, YH., Yu, CM., Pan, ZQ. et al. A gold electrode modified with hemoglobin and the chitosan@Fe3O4 nanocomposite particles for direct electrochemistry of hydrogen peroxide. Microchim Acta 180, 659–667 (2013). https://doi.org/10.1007/s00604-013-0977-8
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DOI: https://doi.org/10.1007/s00604-013-0977-8