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Preparation of magnetic and fluorescent bifunctional chitosan nanoparticles for optical determination of copper ion

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Abstract

We describe a simple method for the synthesis of highly magnetic and fluorescent bifunctional chitosan nanoparticles (MF-CSNPs). Water-soluble and magnetic Fe3O4-chitosan nanoparticles and CdSe quantum dots capped with thioglycolic acid were incorporated into a chitosan matrix via electrostatic interaction. The optical, magnetic, crystallographic and morphological properties of the new nanoparticles were studied by UV-visible, fluorescence, X-ray diffraction and transmission electron microscopy. In addition, MF-CSNPs are found to be a useful probe for the determination of copper ion which acts as a quencher of fluorescence. The relative fluorescence intensity of MF-CSNPs is linearly related to the concentration of copper ion in the 0.125 to 25 ng·mL-1 concentration range. The MF-CSNPs also are found to adsorb copper ion which therefore can be separated and enriched by manipulating them with an external magnetic field. Before enrichment, the limit of detection (LOD) for copper ion is 120 pg·mL-1, but after enrichment, the LOD is 46 pg·mL-1.

High magnetic and fluorescent bifunctional chitosan nanoparticles (MF-CSNPs) have been successfully synthesized via electrostatic interaction. MF-CSNPs are shown to represent a quenchable fluorescent probe for the detection of copper ion in water solution.

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Acknowledgement

This work is supported by Development Program of China (863 program, grant number 2007AA06Z418) and the Fundamental Research Funds for the Central Universities. The authors want to thank Center for Electron Microscopy Wuhan University for assistance with the characterization of samples.

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Authors and Affiliations

  1. College of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan, 430072, People’s Republic of China

    Li Liu, Ling Xiao, Huayue Zhu & Xiaowen Shi

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  1. Li Liu
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  2. Ling Xiao
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  3. Huayue Zhu
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  4. Xiaowen Shi
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Correspondence to Ling Xiao.

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Liu, L., Xiao, L., Zhu, H. et al. Preparation of magnetic and fluorescent bifunctional chitosan nanoparticles for optical determination of copper ion. Microchim Acta 178, 413–419 (2012). https://doi.org/10.1007/s00604-012-0855-9

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  • DOI: https://doi.org/10.1007/s00604-012-0855-9

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