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Amperometric nitrite biosensor based on a gold electrode modified with cytochrome c on Nafion and Cu-Mg-Al layered double hydroxides

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Abstract

An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transform infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 μM. The detection limit is 2 × 10−7 M (S/N = 3). The biosensor was successfully applied to the determination of nitrite in food samples.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.20775044) and the Natural Science Foundation of Shandong province, China (Y2006B20).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, Shandong, China

    Huanshun Yin, Tao Liu, Lin Cui, Shiyun Ai & Yanyan Qiu

  2. College of Resources and Environment, Shandong Agricultural University, Taian, 271018, Shandong, China

    Huanshun Yin & Lusheng Zhu

  3. College of Life Science, Beijing Normal University, 100875, Beijing, China

    Yunlei Zhou

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  1. Huanshun Yin
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  2. Yunlei Zhou
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  3. Tao Liu
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  4. Lin Cui
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  5. Shiyun Ai
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  6. Yanyan Qiu
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  7. Lusheng Zhu
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Correspondence to Shiyun Ai or Lusheng Zhu.

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Yin, H., Zhou, Y., Liu, T. et al. Amperometric nitrite biosensor based on a gold electrode modified with cytochrome c on Nafion and Cu-Mg-Al layered double hydroxides. Microchim Acta 171, 385–392 (2010). https://doi.org/10.1007/s00604-010-0444-8

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  • DOI: https://doi.org/10.1007/s00604-010-0444-8

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