Abstract
Bone allografts can be treated by various techniques before implantation. Recently, treatments based on supercritical carbon dioxide (CO2) were developed. The goal of this study was to evaluate the influence of such a treatment on the biomechanical properties of bone allografts. Thirteen human femoral heads obtained from patients who had undergone hip arthroplasty were cut along the frontal plane yielding to two slices with similar mechanical properties. For each femoral head, one of the two slices (randomly chosen) was fresh-frozen, whereas the other one underwent all steps of a supercritical CO2 based treatment in order to clean and secure bone tissue. Nine specimens (7 mm×9 mm×10 mm) per slice were then cut and loaded under compression in a physiologic saline solution maintained at 37°C. For the maximal compressive strength a mean value (SD) of 9.6 (2.4) MPa for fresh bone and 10.2 (5.2) MPa for treated one was found. Regarding the Young’s modulus a mean value of 417 (85) MPa was obtained for fresh specimens and 412 (149) MPa for the treated ones. No statistical difference was found between the bone specimens treated with supercritical CO2 and the fresh-frozen paired specimens when considering maximal compressive strength, Young’s modulus and work to failure.
Résumé
Les allogreffes osseuses peuvent être traitées par différents procédés avant implantation. Récemment le dioxyde de carbone (CO2) supercritique a été utilisé comme base d’un traitement. Le but de cette étude était d’évaluer l‘influence d’un tel procédé sur les propriétés biomécaniques d’allogreffes osseuses. Treize têtes fémorales, provenant de patients ayant eu une arthroplastie totale de hanche, ont été découpées selon le plan frontal en deux tranches ayant des propriétés mécaniques similaires. Pour chaque tête fémorale, une des deux tranches (de façon aléatoire) était congelée fraîche, alors que l’autre subissait les différentes étapes du traitement utilisant le CO2 supercritique. Neuf échantillons (7 mm×9 mm×10 mm) par tranche ont été découpés puis sollicités en compression dans un bain de sérum physiologique maintenu à 37°C. Pour la contrainte maximale en compression, la moyenne (écart type) est de 9.6 (2.4) MPa pour l’os frais et de 10.2 (5.2) MPa pour l’os traité. Quant au module d’Young les valeurs sont de 417 (85) MPa pour les échantillons frais et de 412 (149) MPa pour les échantillons traités. Aucune différence statistique, n’a été trouvée pour des échantillons traités par CO2supercritique par rapport à des échantillons appariés, frais et congelés, lorsque l’on considère la contrainte maximale à la rupture, le module d’élasticité et l‘énergie emmagasinée à la rupture.
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Acknowledgements
The authors are deeply grateful to I. Kojadinovic for his help in the data analysis. This study was partly funded by Biobank Company.
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Mitton, D., Rappeneau, J. & Bardonnet, R. Effect of a supercritical CO2 based treatment on mechanical properties of human cancellous bone. Eur J Orthop Surg Traumatol 15, 264–269 (2005). https://doi.org/10.1007/s00590-005-0250-x
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DOI: https://doi.org/10.1007/s00590-005-0250-x