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Effect of dietary supplementation of bentonite and yeast cell wall on serum endotoxin, inflammatory parameters, serum and milk aflatoxin in high-producing dairy cows during the transition period

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Abstract

Twenty-four Holstein cows were randomly allocated to four equal experimental groups containing a control (n = 6) and three treatment (n = 6) groups. Experimental animals were selected according to their average milk yield, weight, and body condition score. The experimental groups received basal diet + Saccharomyces Cerevisiae cell wall (SCW) (group 1), basal diet + bentonite + SCW (group 2), basal diet + bentonite (group 3), and basal diet (control), on a dry matter basis (group 4). Blood samples were taken 4, 3, and 1 week prior to and 1 and 3 weeks following parturition. Serum concentrations of haptoglobin (Hp), serum amyloid A (SAA), lipopolysaccharide (LPS), and concentration of aflatoxin B1 in ration and M1 in milk and blood were determined during the transition period. The results showed that LPS concentration in blood was in the normal range in all groups. The acute-phase proteins (Hp and SAA) were in the normal range as well. The concentration of aflatoxin M1 in the serum of the control group was higher than other groups, yet lower in the group receiving toxin binders (p < 0.05). The mean aflatoxin M1 concentration in milk was the lowest in the group receiving SCW (p < 0.05). Similarly, the concentration of aflatoxin M1 in milk was the highest in the control group (p < 0.05). The study also showed that the levels of endotoxin in ration of different groups resulted from our experimental diet were not high enough to stimulate APP response. It was further observed that SCW might be able to reduce the level of aflatoxin in serum and milk. The results obtained from our study may lead to a better understanding of using dietary supplements, such as bentonite and yeast cell wall, during the transitional period. Moreover, the study may contribute to our knowledge on simultaneous LPS and toxin binders for providing more safe food during the transition period in cattle.

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Acknowledgments

The authors would like to thank MoghufatMalek industry for their provision of cows, especially Mr. Miri, Mr. Naghavi, and Mr. Ershadi.

Funding

This study was financed by PhD student project grant by School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

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Authors and Affiliations

  1. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 7144169155, Iran

    Ahmadreza Mirzaei, Ali Hajimohammadi, Khalil Badiei, Mehrdad Pourjafar & Seyed Amin Razavi

  2. Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Abbas Ali Naserian

Authors
  1. Ahmadreza Mirzaei
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  2. Ali Hajimohammadi
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  3. Khalil Badiei
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  4. Mehrdad Pourjafar
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  5. Abbas Ali Naserian
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  6. Seyed Amin Razavi
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Correspondence to Ali Hajimohammadi.

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All animals were treated in accordance with the regulations on the guidelines of the Iranian Council of Animal Care (1995), and the experiment was approved by the Institutional Animal Care Committee for Animals Used in Research.

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Mirzaei, A., Hajimohammadi, A., Badiei, K. et al. Effect of dietary supplementation of bentonite and yeast cell wall on serum endotoxin, inflammatory parameters, serum and milk aflatoxin in high-producing dairy cows during the transition period. Comp Clin Pathol 29, 433–440 (2020). https://doi.org/10.1007/s00580-019-03074-y

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