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Effects of silver nanoparticles (AgNPs) on hematological parameters of rainbow trout, Oncorhynchus mykiss

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Abstract

Silver nanoparticles (AgNPs) are potentially toxic materials that are used today in numerous consumer products. Releasing of AgNPs to aquatic environment may affect adversely aquatic animal health including fish. In this study, we examined the effects of AgNPs on hematological parameters of rainbow trout, Oncorhynchus mykiss. The parameters include red blood cells (RBCs) and hemoglobin (Hb), hematocrit (Hct), white blood cells (WBCs), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein (TP), and albumin (AL). Three treatment groups included fish treated with 0.1 mg/l AgNP solution (T1), 0.2 mg/l AgNP (T2), and 0.4 mg/l AgNP (T3), and one group without AgNP treatment was considered as control group. In the fourth and eighth days of the experiment, the values of RBCs and WBCs were higher in all treatments than those in the control group (P < 0.05). The Hct in the T3 group showed lower values than that of the control group in the eighth day (P < 0.05). Although the Hb concentrations were higher in the T2 and T3 groups than those in the control group in the first blood sampling, a significant decrease compared to the control group was observed in Hb values in the second sampling for the T3 group (P < 0.05). The MCV and MCH in the treatment groups decreased as the concentration of AgNPs increased (P < 0.05). In contrast, the MCHC values showed an increasing trend as the concentration of AgNPs increased (P < 0.05). The concentrations of liver enzymes (i.e., ALT, AST, ALP, and LDH) were higher in all treatment groups than those in the control group (P < 0.05). The TP and AL concentrations decreased compared to the control group as the concentration of AgNPs increased (P < 0.05). Our results demonstrated that silver nanoparticles induce significant changes in hematologic parameters of rainbow trout.

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References

  • Atamanalp M, Aksakal E, Kocaman EM, Ucar A, Şisman T, Turkez H (2011) The alterations in the hematological parameters of rainbow trout, Oncorhynchus mykiss, exposed to cobalt chloride. Kafkas Univ Vet Fak Derg 17:S73–S76

    Google Scholar 

  • Benarjee G, Narayana Rao B, Srikanth K, Ramu G (2010) Haematological changes in fresh water fish, Channa punctatus due to the effect of rayon industry effluents. Pollut Res 29:63–68

    CAS  Google Scholar 

  • Böckmann J, Lahl H, Eckert T, Unterhalt B (2000) Titan-Blutspiegel vor und nach Belastungsversuchen mit Titandioxid. Pharmazie 55:140–143

    PubMed  Google Scholar 

  • Braydich-Stolle L, Hussain S, Schlager J, Hofmann MC (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci 88:412–419

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Chen HW, Su SF, Chien CT, Lin WH, Yu SL, Chou CC, Chen JJ, Yang PC (2006) Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. Fed Am Soc Exp Biol J 20:2393–2395

    CAS  Google Scholar 

  • Cheraghi J, Hosseini E, Hoshmandfar R, Sahraei R (2013) Hematologic parameters study of male and female rats administrated with different concentrations of silver nanoparticles. Int J Agric and J Crop Sci 5:789–796

  • Duomas BT, Bayso DD, Carter RJ, Peters T, Schaffer R (1981) Determination of total serum protein. Clin Chem 27:1642–1643

    Google Scholar 

  • Homatowska A, Wojtaszek J, Adamowicz A (2002) Haematological indices and circulating blood picture in the sunbleak, Leucaspius delineatus (Heckel, 1843). Zool Pol 47:57–68

    Google Scholar 

  • Hussain SM, Hess KL, Gearhart JM, Geiss K, Schlager JJ (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–986

    Article  CAS  PubMed  Google Scholar 

  • Hussain SM, Javorina AK, Schrand AM, Duhart HM, Ali SF, Schlager JJ (2006) The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicol Sci 92:456–463

    Article  CAS  PubMed  Google Scholar 

  • Ji JH, Jung JH, Kim SS, Yoon JU, Park JD, Choi BS, Chung YH, Kwon IH, Jeong J, Han BS, Shin JH, Sung JH, Song KS, Yu IJ (2007) Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhal Toxicol 19:857–871

    Article  CAS  PubMed  Google Scholar 

  • Joshi PK, Bose M, Harish D (2002) Haematological changes in the blood of Clarias batrachus exposed to mercuric chloride. J Ecotoxicol Environ Monit 12:119–122

    CAS  Google Scholar 

  • Miao AJ, Schwehr KA, Xu C, Zhang SJ, Luo Z, Quigg A, Santschi PH (2009) The algal toxicity of silver engineered nanoparticles and detoxification by exopolymeric substances. Environ Pollut 157:3034–3041

    Article  CAS  PubMed  Google Scholar 

  • Navarro E, Piccapietra F, Wagner B, Marconi F, Kaegi R, Odzak N, Sigg L, Behra R (2008) Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environ Sci Technol 42:8959–8964

    Article  CAS  PubMed  Google Scholar 

  • Neskovic NK, Elezovic I, Karan V, Poleksic V, Budimir M (1993) Acute and subacute toxicity of atrazine to carp (Cyprinus carpio L.). Ecotoxicol Environ Saf 25:173–182

    Article  CAS  PubMed  Google Scholar 

  • Ololade IA, Oginni O (2010) Toxic stress and hematological effects of nickel on African catfish, Clarias gariepinus, fingerlings. J Environ Chem Ecotoxicol 22:14–19

    Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83

    Article  CAS  PubMed  Google Scholar 

  • Ramesh M, Srinivasan R, Saravanan M (2009) Effect of atrazine (Herbicide) on blood parameters of common carp Cyprinus carpio (Actinopterygii: Cypriniformes). Afr J Environ Sci Technol 3:453–458

    CAS  Google Scholar 

  • Rehulka J (2003) Haematological and biochemical analyses in rainbow trout Oncorhynchus mykiss affected by viral haemorrhagic septicaemia (VHS). Dis Aquat Org 56:186–193

    Article  Google Scholar 

  • Reinhold DG (1953) The principle diseases of lower vertebrates. Academic Press, London, 600 pp

    Google Scholar 

  • Satheeshkumar P, Ananthan G, Senthil Kumar D, Jagadeesan L (2012) Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India. Comp Clin Pathol 21:1187–1191

    Article  CAS  Google Scholar 

  • Tencalla GF, Dietrich RD, Schlatter CH (1994) Toxicity of Microcystis aeruginosa peptide toxin to yearling rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 30:215–224

    Article  CAS  Google Scholar 

  • Yacobi NR, Phuleria HC, Demaio L, Liang CH, Peng CA, Sioutas C, Borok Z, Kim KJ, Crandall ED (2007) Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties. Toxicol In Vitro 21:1373–1381

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Mostafa Halimi.

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Imani, M., Halimi, M. & Khara, H. Effects of silver nanoparticles (AgNPs) on hematological parameters of rainbow trout, Oncorhynchus mykiss . Comp Clin Pathol 24, 491–495 (2015). https://doi.org/10.1007/s00580-014-1927-5

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  • DOI: https://doi.org/10.1007/s00580-014-1927-5

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