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Localization of proton-ATPase genes expressed in arbuscular mycorrhizal tomato plants

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Abstract

In arbuscular mycorrhizal symbioses, solutes such as phosphate are transferred to the plant in return for photoassimilates. The uptake mechanism is probably facilitated by a proton gradient generated by proton H+-ATPases. We investigated expression of Lycopersicon esculentum Mill. H+-ATPases in mycorrhizal and non-mycorrhizal plants to determine if any are specifically regulated in response to colonization. Tissue expression and cellular localization of H+-ATPases were determined by RNA gel blot analysis and in situ hybridization of mycorrhizal and non-mycorrhizal roots. LHA1, LHA2, and LHA4 had high levels of expression in roots and were expressed predominantly in epidermal cells. LHA1 and LHA4 were also expressed in cortical cells containing arbuscules. The presence of arbuscules in root sections was correlated with lower levels of expression of these two isoforms in the epidermis. These results suggest that LHA1 and LHA4 expression is decreased in epidermal cells located in regions of the root that contain arbuscules. This provides evidence of differential regulation between molecular mechanisms involved in proton-coupled nutrient transfer either from the soil or fungus to the plant.

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Acknowledgment

The financial support of the Australian Research Council is gratefully acknowledged by its contribution of an ARC Large Grant (D.P.S., S.E.S., and F.A.S.). Special thanks to Rosemary White for support with microscopy, to Megan C. Shelden for technical help, and to Ellen Marsh for developing methods for RNA extraction from tomato mycorrhizal roots.

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Authors and Affiliations

  1. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Garry M. Rosewarne

  2. Soil and Land Systems, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, 5005, SA, Australia

    F. Andrew Smith & Sally E. Smith

  3. Donald Danforth Plant Science Center, 975 N. Warson Road, St. Louis, MO, 63132, USA

    Daniel P. Schachtman

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  1. Garry M. Rosewarne
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  2. F. Andrew Smith
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  3. Daniel P. Schachtman
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  4. Sally E. Smith
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Correspondence to Garry M. Rosewarne.

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Rosewarne, G.M., Smith, F.A., Schachtman, D.P. et al. Localization of proton-ATPase genes expressed in arbuscular mycorrhizal tomato plants. Mycorrhiza 17, 249–258 (2007). https://doi.org/10.1007/s00572-006-0101-6

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