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Polymorphisms in interleukin-4-related genes in patients with minimal change nephrotic syndrome

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Abstract

Minimal change nephrotic syndrome (MCNS) in children is frequently associated with allergy and immunoglobulin E production. T helper subtype 2 cytokines, such as interleukin (IL)-4 and IL-13, may have an important role in the development of atopy. We investigated the association of genetic variations of IL-4 receptor α chain (IL-4Rα), IL-13 and signal transducer and activator of transcription 6 (STAT6) genes with MCNS. We analyzed these polymorphisms in 85 Japanese children (55 males, 30 females) with MCNS and 127 healthy controls with neither allergic nor renal diseases. Genomic DNA was extracted from peripheral blood leukocytes. The single nucleotide polymorphisms of IL-4Rα (Ile50Val) and IL-13 (R130Q) were detected by primer-specific polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism analysis, respectively. GT repeat polymorphism in STAT6 gene exon 1 was investigated by fragment length analysis. A significant difference in allelic frequencies in the STAT6 gene was detected between the MCNS and control groups. There was no significant difference between the two groups for genetic variations of IL-4Rα and IL-13 genes. We found a significant difference in IL-4Rα gene polymorphism between MCNS subgroups divided according to the number of relapses. These results suggested that the genetic variation in the first exon of the STAT6 gene may be associated with a predisposition to MCNS and that the genetic variation in the IL-4Rα gene may be associated with its clinical course.

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Acknowledgments

We thank Mrs. Tomoko Endo and Mrs. Chinori Iijima for technical assistance. This work was supported in part by Health Science Research Grants (Research on Eye and Ear Science, Immunology, Allergy and Organ Transplantation) from the Ministry of Health Labor and Welfare of Japan.

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Authors and Affiliations

  1. Department of Pediatrics and Developmental Medicine, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Yuka Ikeuchi, Yasuko Kobayashi, Hirokazu Arakawa, Michiko Suzuki, Kazushi Tamra & Akihiro Morikawa

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  1. Yuka Ikeuchi
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  2. Yasuko Kobayashi
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  3. Hirokazu Arakawa
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  4. Michiko Suzuki
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  5. Kazushi Tamra
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  6. Akihiro Morikawa
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Correspondence to Yasuko Kobayashi.

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Ikeuchi, Y., Kobayashi, Y., Arakawa, H. et al. Polymorphisms in interleukin-4-related genes in patients with minimal change nephrotic syndrome. Pediatr Nephrol 24, 489–495 (2009). https://doi.org/10.1007/s00467-008-1003-y

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  • DOI: https://doi.org/10.1007/s00467-008-1003-y

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