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Fabrication, characterization and application of laccase–nylon 6,6/Fe3+ composite nanofibrous membrane for 3,3′-dimethoxybenzidine detoxification

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Abstract

In this study, laccase was immobilized on nylon 6,6/Fe3+ composite (NFC) nanofibrous membrane and used for the detoxification of 3,3′-dimethoxybenzidine (DMOB). The average size and tensile strength of the NFC membrane were found to be 60–80 nm (diameter) and 2.70 MPa, respectively. The FTIR results confirm that the amine (N–H) group of laccase was attached with Fe3+ particles and the carbonyl (C=O) group of NFC membrane via hydrogen bonding. The half-life of the laccase–NFC membrane storage stability was increased from 6 to 11 weeks and the reusability was significantly extended up to 43 cycles against ABTS oxidation. Enhanced electro-oxidation of DMOB by laccase was observed at 0.33 V and the catalytic current was found to be 30 µA. The DMOB-treated mouse fibroblast 3T3-L1 preadipocytes showed maximum (97 %) cell inhibition at 75 µM L−1 within 24 h. The cytotoxicity of DMOB was significantly decreased to 78 % after laccase treatment. This study suggests that laccase–NFC membrane might be a good candidate for emerging pollutant detoxification.

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References

  1. Jasnowska J, Ligaj M, Stupnicka B, Filipiak M (2004) DNA sensor for o-dianisidine. Bioelectrochemistry 64:85–90

    Article  CAS  Google Scholar 

  2. IARC (1974) 3,3′-Dimethoxybenzidine (o-dianisidine). IARC monographs on the evaluation of carcinogenic risk of chemicals to humans, vol. 4. International Agency for Research on Cancer, Lyon, pp 41–47

  3. NTP (2014) Report on carcinogens, thirteenth Ed. 3,3′-Dimethoxybenzidine and dyes metabolized to 3,3′-dimethoxybenzidine. Department of Health and Human Services, Public Health Service. NC, USA

  4. Sornambikai S, Hin LQ, Marimuthu K, Abdul Kadir MR (2016) Fish embryo toxicity assessment of o-dianisidine in Clarias gariepinus and its electrochemical treatment in aquatic samples using super conductive carbon black. RSC Adv 6:50255–50266

    Article  CAS  Google Scholar 

  5. WHO (2008) Monographs on the evaluation of the carcinogenic risk of chemicals to humans: some aromatic amines, organic dyes, and related exposures. International Agency for Research on Cancer, Lyon, pp 99

  6. US-EPA (1997) Health effects assessment summary tables. Solid waste and emergency response, Office of Emergency and Remedial Response, Cincinnati (EPA/540/R-97-036)

  7. Adnan LA, Sathishkumar P, Mohd Yusoff AR, Hadibarata T (2015) Metabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03. Int Biodeterior Biodegrad 104:274–282

    Article  CAS  Google Scholar 

  8. Carabajal M, Perullini M, Jobbagy M, Ullrich R, Hofrichter M, Levin L (2016) Removal of phenol by immobilization of Trametes versicolor in silica-alginate-fungus biocomposites and loofa sponge. CSAWAC 44:107–218

    Google Scholar 

  9. Agarwal PK (2006) Enzymes: an integrated view of structure, dynamics and function. Microb Cell Fact 5:2

    Article  Google Scholar 

  10. Torres E, Bustos-Jaimes I, Leborgne S (2003) Potential use of oxidative enzymes for the detoxification of organic pollutants. Appl Catal B 46:1–15

    Article  CAS  Google Scholar 

  11. Sathishkumar P, Mythili A, Hadibarata T, Palvannan T, Salim MR, Mohd Yusoff AR (2014) Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes. RSC Adv 4:11689–11697

    Article  CAS  Google Scholar 

  12. Sathishkumar P, Chae JC, Unnithan AR, Palvannan T, Kamala-Kannan S, Oh BT (2012) Laccase-poly(lactic-co-glycolic acid) (PLGA) nanofiber: highly stable, reusable, and efficacious for the transformation of diclofenac. Enzyme Microb Technol 51:113–118

    Article  CAS  Google Scholar 

  13. Spina F, Cordero C, Schiliro T, Sgorbini B, Pignata C, Gilli G, Bicchi C, Varese GC (2015) Removal of micropollutants by fungal laccases in model solution and municipal wastewater: evaluation of estrogenic activity and ecotoxicity. J Clean Prod 100:185–194

    Article  CAS  Google Scholar 

  14. Sathishkumar P, Palvannan T, Murugesan K, Kamala-Kannan S (2013) Detoxification of malachite green by Pleurotus florida laccase produced under solid-state fermentation using agricultural residues. Environ Technol 34:139–147

    Article  CAS  Google Scholar 

  15. Asgher M, Shahid M, Kamal S, Iqbal HMN (2014) Recent trends and valorization of immobilization strategies and ligninolytic enzymes by industrial biotechnology. J Mol Catal B-Enzym 101:56–66

    Article  CAS  Google Scholar 

  16. Liu Y, Cui L, Guan F, Gao Y, Hedin NE, Zhu L, Fong H (2007) Crystalline morphology and polymorphic phase transitions in electrospun nylon-6 nanofibers. Macromolecules 40:6283–6290

    Article  CAS  Google Scholar 

  17. Ikeda K, Umeno D, Saito K, Koide F, Miyata E, Sugo T (2011) Removal of boron using nylon-based chelating fibers. Ind Eng Chem Res 50:5727–5732

    Article  CAS  Google Scholar 

  18. Miyazawa T, Asari Y, Miyoshi K, Umeno D, Saito K, Nagatani T, Yoshikawa N (2009) Development of novel ion-exchange membranes for electrodialysis of sea water by electron-beam-induced graft polymerization. II. Graft polymerization of vinyl benzyltrimethylammonium chloride and sodium styrenesulfonate onto nylon-6 film. Bull Soc Sea Water Sci Jpn 63:175–183

    CAS  Google Scholar 

  19. Wolfenden BS, Wilson RL (1982) Radical cations as reference chromogens in studies of one-electron transfer reactions: pulse radio analysis studies of ABTS. J Chem Soc Perkin Trans 2:805–812

    Article  Google Scholar 

  20. Sathishkumar P, Kamala-Kannan S, Cho M, Kim JS, Hadibarata T (2014) Laccase immobilization on cellulose nanofiber: the catalytic efficiency and recyclic application for simulated dye effluent treatment. J Mol Catal B-Enzym 100:111–120

    Article  CAS  Google Scholar 

  21. Gao J, Li W, Shi H, Hu M, Li RKY (2014) Preparation, morphology, and mechanical properties of carbon nanotube anchored polymer nanofiber composite. Compos Sci Technol 92:95–102

    Article  CAS  Google Scholar 

  22. Wang ZG, Wan LS, Liu ZM, Huang XJ, Xu ZK (2009) Enzyme immobilization on electrospun polymer nanofibers: an overview. J Mol Catal B-Enzym 56:189–195

    Article  CAS  Google Scholar 

  23. Xu R, Tang R, Zhou Q, Li F, Zhang B (2015) Enhancement of catalytic activity of immobilized laccase for diclofenac biodegradation by carbon nanotubes. Chem Eng J 262:88–95

    Article  CAS  Google Scholar 

  24. Fatarella E, Spinelli D, Ruzzante M, Pogni R (2014) Nylon 6 film and nanofiber carriers: preparation and laccase immobilization performance. J Mol Catal B-Enzym 102:41–47

    Article  CAS  Google Scholar 

  25. Rekuć A, Kruczkiewicz P, Jastrzembska B, Liesiene J, Peczyńska-Czoch W, Bryjak J (2008) Laccase immobilization on the tailored cellulose-based Granocel carriers. Int J Biol Macromol 42:208–215

    Article  Google Scholar 

  26. Bautista LF, Morales G, Sanz R (2010) Immobilization strategies for laccase from Trametes versicolor on mesostructured silica materials and the application to the degradation of naphthalene. Bioresour Technol 101:8541–8548

    Article  Google Scholar 

  27. Ahn MY, Zimmerman AR, Martınez CE, Archibald DD, Bollag JM, Dec J (2007) Characteristics of Trametes villosa laccase adsorbed on aluminum hydroxide. Enzyme Microb Technol 41:141–148

    Article  CAS  Google Scholar 

  28. Xu R, Si Y, Wu X, Li F, Zhang B (2014) Triclosan removal by laccase immobilized on mesoporous nanofibers: strong adsorption and efficient degradation. Chem Eng J 255:263–270

    Article  Google Scholar 

  29. Wan LS, Ke BB, Xu ZK (2008) Electrospun nanofibrous membranes filled with carbon nanotubes for redox enzyme immobilization. Enzyme Microb Technol 42:332–339

    Article  CAS  Google Scholar 

  30. Rahmani K, Faramarzi MA, Mahvi AH, Gholami M, Esrafili A, Forootanfar H, Farzadkia M (2015) Elimination and detoxification of sulfathiazole and sulfamethoxazole assisted by laccase immobilized on porous silica beads. Int Biodeterior Biodegrad 97:107–114

    Article  CAS  Google Scholar 

  31. Huang XJ, Chen PC, Huang F, Ou Y, Chen MR, Xu ZK (2011) Immobilization of Candida rugosa lipase on electrospun cellulose nanofiber membrane. J Mol Catal B-Enzym 70:95–100

    Article  CAS  Google Scholar 

  32. Sampaio LMP, Padrao J, Jorge F, Silva JP, Silva CJ, Dourado F, Zille A (2016) Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties. Carbohyd Polym 145:1–12

    Article  CAS  Google Scholar 

  33. Bourbonnais R, Leech D, Paice MG (1998) Electrochemical analysis of the interactions of laccase mediators with lignin model compounds. Biochim Biophys Acta 1379:381–390

    Article  CAS  Google Scholar 

  34. Sundaram S, Jagannathan M, Abdul Kadir MR, Palanivel S, Hadibarata T, Mohammed Yusoff AR (2015) A new electro-generated o-dianisidine derivative stabilized MWCNT-modified GCE for low potential gallic acid detection. RSC Adv 5:45996–46006

    Article  CAS  Google Scholar 

  35. Wen YH, Zhang HM, Qian P, Zhou HT, Zhao P, Yi BL, Yang YS (2006) Studies on iron (Fe3+/Fe2+)-complex/bromine (Br2/Br−) redox flow cell in sodium acetate solution. J Electrochem Soc 153:A929–A934

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Ministry of Higher Education (Malaysia) for the LRGS 4L810 (Grant No. 203/PKT/6720006) and Universiti Teknologi Malaysia.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia

    M. Jasmin Fathi Jasni, Abdull Rahim Mohd Yusoff & Nor Aziah Buang

  2. Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia

    Palanivel Sathishkumar & Zulkifli Yusop

  3. Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia

    Sundaram Sornambikai & Mohammed Rafiq Abdul Kadir

  4. Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia

    Abdull Rahim Mohd Yusoff

  5. Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Fuad Ameen

Authors
  1. M. Jasmin Fathi Jasni
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  2. Palanivel Sathishkumar
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  3. Sundaram Sornambikai
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  4. Abdull Rahim Mohd Yusoff
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  5. Fuad Ameen
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  6. Nor Aziah Buang
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  7. Mohammed Rafiq Abdul Kadir
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  8. Zulkifli Yusop
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Correspondence to Palanivel Sathishkumar or Abdull Rahim Mohd Yusoff.

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Jasni, M.J.F., Sathishkumar, P., Sornambikai, S. et al. Fabrication, characterization and application of laccase–nylon 6,6/Fe3+ composite nanofibrous membrane for 3,3′-dimethoxybenzidine detoxification. Bioprocess Biosyst Eng 40, 191–200 (2017). https://doi.org/10.1007/s00449-016-1686-6

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  • DOI: https://doi.org/10.1007/s00449-016-1686-6

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