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The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues

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Abstract

The lignin-modifying enzymes (LMEs) play an important role in decomposition of agricultural residues, which contain a certain amount of lignin. In this study, the production of LMEs by three co-cultivated combinations of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora and the respective monocultures was comparatively investigated. Laccase and manganese peroxidases (MnP) were significantly promoted in the co-culture of P. radiata and D. squalens, and corncob was verified to be beneficial for laccase and MnP production. Moreover, laccase production by co-culture of P. radiata and D. squalens with high ratio of glucose to nitrogen was higher than low ratio under carbon- and nitrogen-meager conditions. New laccase isoenzymes measured by Native-PAGE were stimulated by co-cultured P. radiata with D. squalens or C. subvermispora, respectively, growing in the defined medium containing corncob, but the expression of laccase was greatly restrained by the co-culturing of D. squalens with C. subvermispora. This study showed that the synergistic and depressing effects of co-cultivation of P. radiata, D. squalens and C. subvermispora on LMEs were species specific.

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Abbreviations

LMEs:

Lignin-modifying enzymes

MnP:

Manganese peroxidases

LiP:

Lignin peroxidase

VP:

Versatile peroxidase

ABTS:

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt

VA:

Veratryl alcohol

PR:

Phlebia radiata

DS:

Dichomitus squalens

CS:

Ceriporiopsis subvermispora

PDA:

Potato dextrose agar

DNS:

3,5-Dinitrosalicylic acid

Native-PAGE:

Native polyacrylamide gel electrophoresis

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Acknowledgments

This study was financially supported by the Natural Science Foundation of Zhejiang Province, China (Y3090026).

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, China

    Ya-Chen Dong, Ming-Liang Fu & Qi-He Chen

  2. Institute of Quality and Standard for Agriculture Products, Zhejiang Academy of Agriculture Sciences, Hangzhou, 310021, China

    Wei Wang

  3. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China

    Zhong-Ce Hu

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  1. Ya-Chen Dong
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  2. Wei Wang
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  3. Zhong-Ce Hu
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Corresponding author

Correspondence to Qi-He Chen.

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The authors Y.-C. Dong and W. Wang contributed equally to this work.

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Dong, YC., Wang, W., Hu, ZC. et al. The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues. Bioprocess Biosyst Eng 35, 751–760 (2012). https://doi.org/10.1007/s00449-011-0655-3

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