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Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film

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Abstract

Polyacrylonitrile film (PAN) surfaces were modified with chemical polymerization of conductive polyaniline (PANI) in the presence of potassium dichromate as an oxidizing agent. The conductive films were used for immobilization of uricase. The surface resistance of the conductive film in this work was found to be 0.97 kΩ/cm. The maximum amount of immobilized enzyme on conductive film containing 2.4% PANI was about 216 μg/cm2. The optimum pH for free and immobilized enzymes was observed at 7.0 and 7.5, respectively. The K m values for free and immobilized uricase were found to be 94 and 138 μM, respectively. V max values were calculated as 1.87 and 1.63 U/mg protein for the free and immobilized enzymes, respectively. Immobilized uricase exhibited ~68% of its original activity even after 2 months of storage at 4 °C while the free enzyme lost its initial activity within 4 weeks.

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Authors and Affiliations

  1. Biochemical Processing and Biomaterial Research Laboratory, Department of Chemistry and Biology, Faculty of Arts and Sciences, Gazi University, 06500, Teknikokullar, Ankara, Turkey

    Gülay Bayramoğlu & M. Yakup Arıca

  2. Department of Environmental Sciences, Institute of Science and Technology, Gazi University, 06570, Ankara, Turkey

    Begüm Altıntaş

Authors
  1. Gülay Bayramoğlu
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  2. Begüm Altıntaş
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  3. M. Yakup Arıca
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Correspondence to Gülay Bayramoğlu.

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Bayramoğlu, G., Altıntaş, B. & Arıca, M.Y. Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film. Bioprocess Biosyst Eng 34, 127–134 (2011). https://doi.org/10.1007/s00449-010-0453-3

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  • DOI: https://doi.org/10.1007/s00449-010-0453-3

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