Skip to main content

Advertisement

SpringerLink
Log in

Nanoparticles in the fight against mosquito-borne diseases: bioactivity of Bruguiera cylindrica-synthesized nanoparticles against dengue virus DEN-2 (in vitro) and its mosquito vector Aedes aegypti (Diptera: Culicidae)

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

An Erratum to this article was published on 28 September 2015

Abstract

Mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. Dengue is a viral disease mainly vectored through the bite of Aedes mosquitoes. Its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. There is no specific treatment for dengue. Its prevention and control solely depend on effective vector control measures. Mangrove plants have been used in Indian traditional medicine for a wide array of purposes. In this research, we proposed a method for biosynthesis of antiviral and mosquitocidal silver nanoparticles (AgNP) using the aqueous extract of Bruguiera cylindrica leaves. AgNP were characterized using a variety of biophysical analyses, including UV-visible spectrophotometry, Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Bruguiera cilyndrica aqueous extract and green-synthesized AgNP were tested against the primary dengue vector Aedes aegypti. AgNP were the most effective. LC50 values ranged from 8.93 ppm (larva I) to 30.69 ppm (pupa). In vitro experiments showed that 30 μg/ml of AgNP significantly inhibited the production of dengue viral envelope (E) protein in vero cells and downregulated the expression of dengue viral E gene. Concerning nontarget effects, we observed that the predation efficiency of Carassius auratus against A. aegypti was not affected by exposure at sublethal doses of AgNP. Predation in the control was 71.81 % (larva II) and 50.43 % (larva III), while in an AgNP-treated environment, predation was boosted to 90.25 and 76.81 %, respectively. Overall, this study highlights the concrete potential of green-synthesized AgNP in the fight against dengue virus. Furthermore, B. cylindrica-synthesized AgNP can be employed at low doses to reduce larval and pupal population of A. aegypti, without detrimental effects of predation rates of mosquito predators, such as C. auratus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Ahmad A, Mukherjee M, Mandal D, Senapati S, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Coll Surf B Biointerf 28:313–318

    Article  CAS  Google Scholar 

  • Amer A, Mehlhorn H (2006a) Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitol Res 99:478–490

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006b) The sensilla of Aedes and Anopheles mosquitoes and their importance in repellency. Parasitol Res 99:491–499

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006c) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006d) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477

    Article  PubMed  Google Scholar 

  • Amerasan D, Nataraj T, Murugan K, Madhiyazhagan P, Panneerselvam C, Nicoletti M, Benelli G (2015) Mico-synthesis of silver nanoparticles using Metarhizium anisopliae against the rural malaria vector Anopheles culicifacies Giles (Diptera: Culicidae). J Pest Sci. doi:10.1007/s10340-015-0675-x

    Google Scholar 

  • Azizullah A, Rehman ZU, Ali I, Murad W, Muhammad N, Ullah W, Hader D-P (2014) Chlorophyll derivatives can be an efficient weapon in the fight against dengue. Parasitol Res 113:4321–4326

    Article  PubMed  Google Scholar 

  • Bar H, Dipak Bhui KR, Gobinda Sahoo P, Sarkar P (2009) Green synthesis of silver nanoparticles using latex of Jatropha curcas. Cool Surf A Physicochem Eng Asp 339:134–139

    Article  CAS  Google Scholar 

  • Baram-Pinto D, Shukla S, Perkas N, Gedanken A, Sarid R (2009) Inhibition of herpes simplex virus type 1 infection by silver nanoparticles capped with mercaptoethane sulfonate. Bioconjugate Chem 20:1497–1502

    Article  CAS  Google Scholar 

  • Benelli G (2015a) Research in mosquito control: current challenges for a brighter future. Parasitol Res. doi:10.1007/s00436-015-4586-9

    Google Scholar 

  • Benelli G (2015b) Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. Parasitol Res 114:3201–3212

  • Benelli G (2015c) Plant-synthesized nanoparticles in the fight against mosquito vectors: an eco-friendly tool against mosquito vectors? In: “Nanoparticles in the fight against parasites” (Ed. Heinz Mehlhorn), Parasitology Research Monographs, Springer, ISSN: 2192-3671, in press

  • Benelli G, Bedini S, Cosci F, Toniolo C, Conti B, Nicoletti M (2015a) Larvicidal and ovideterrent properties of neem oil and fractions against the filariasis vector Aedes albopictus (Diptera: Culicidae): a bioactivity survey across production sites. Parasitol Res 114:227–236

    Article  PubMed  Google Scholar 

  • Benelli G, Bedini S, Flamini G, Cosci F, Cioni PL, Amira S, Benchikh F, Laouer H, Di Giuseppe G,Conti B (2015b) Mediterranean essential oils as effective weapons against the West Nile vector Culex pipiens and the Echinostoma intermediate host Physella acuta: what happens around? An acute toxicity survey on non-target mayflies. Parasitol Res doi: 101007/s00436-014-4267-0

  • Benelli G, Murugan K, Panneerselvam C, Madhiyazhagan P, Conti B, Nicoletti M (2015c) Old ingredients for a new recipe? Neemcake, a low-cost botanical by-product in the fight against mosquito-borne diseases. Parasitol Res 114:391–397

    Article  PubMed  Google Scholar 

  • Borkow G, Lapidot A (2005) Multi-targeting the entrance door to block HIV-1. Curr Drug Targ Infect Disord 5:3–15

    Article  CAS  Google Scholar 

  • Bowatte G, Perera P, Senevirathne G, Meegaskumbura S, Meegaskumbura M (2013) Tadpoles as dengue mosquito (Aedes aegypti) egg predators. Biol Control 67:469–474

    Article  Google Scholar 

  • Chandra G, Bhattacharjee I, Chatterjee SN, Ghosh A (2008) Mosquito control by larvivorous fish. Indian J Med Res 127:13–27

    CAS  PubMed  Google Scholar 

  • Chatterjee SN, Chandra G (1997) Laboratory trials on the feeding pattern of Anopheles subpictus, Culex quinquefasciatus and Armigeres subalbatus larvae by Gambusia affinis. Sci Cult 63:51–52

    Google Scholar 

  • Chitra G, Balasubramani G, Ramkumar R, Sowmiya R, Perumal P (2015) Mukia maderaspatana (Cucurbitaceae) extract-mediated synthesis of silver nanoparticles to control Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Parasitol Res, doi: 101007/s00436-015-4320-7

  • Cho K, Park J, Osaka T, Park S (2005) The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta 51:956–960

    Article  CAS  Google Scholar 

  • Dinesh D, Murugan K, Madhiyazhagan P, Panneerselvam C, Nicoletti M, Jiang W, Benelli G, Chandramohan B, Suresh U (2015) Mosquitocidal and antibacterial activity of green-synthesized silver nanoparticles from Aloe vera extracts: towards an effective tool against the malaria vector Anopheles stephensi? Parasitol Res 114:1519–1529

    Article  PubMed  Google Scholar 

  • Xiang DX, Chen Q, Pang L, Zheng CL (2011) Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods 178:37–142

    Article  Google Scholar 

  • Dubey M, Bhadauria S, Kushwah BS (2009) Green synthesis of nanosilverparticles from extract of Eucalyptus hybrida (safeda) leaf. Dig J Nanomat Biostruc 4:537–43

    Google Scholar 

  • Elechiguerra JL, Burt JL, Morones JR, Camacho-Bragado A, Gao X, Lara HH, Yacaman MJ (2005) Interaction of silver nanoparticles with HIV-1. J Nanobiotech 3:6

    Article  Google Scholar 

  • Finney DJ (1971) Probit analysis. Cambridge University Press, London, pp 68–78

    Google Scholar 

  • Floore TG (2006) Mosquito larval control practices: past and present. J Am Mosq Control 22:527–533

    Article  CAS  Google Scholar 

  • Gabaldon A (1969) Global eradication of malaria: changes of strategy and future outlook. Am J Trop Med Hyg 18:64–56

    Google Scholar 

  • Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons G, Troiani H, Jose-Yacaman M (2003) Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir 19:1357–1361

    Article  CAS  Google Scholar 

  • Gerberich JB, Laird M (1985) Larvivorous fish in the biocontrol of mosquitoes, with a selected bibliography of recent literature. In: Laird M, Miles JW (eds) Integrated mosquito control methodologies Vol 2 Biocontrol and other innovative components and future directions. Academic Press, London, pp 47–76

    Google Scholar 

  • Ghosh SK, Tiwari SN, Sathyanarayan TS, Sampath TR, Sharma VP, Nanda N, Joshi H, Adak T, Subbarao S (2005) Larvivorous fish in wells target the malaria vector sibling species of the Anopheles culicifacies complex in villages in Karnataka, India. Trans Royal Soc Trop Med Hyg 99:101–105

    Article  CAS  Google Scholar 

  • Goncalvez AP, Engle RE, St Claire M, Purcell RH, Lai CJ (2007) Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proc Natl Acad Sci USA 104(22):9422–9427

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Goodsell DS (2004) Bionanotechnology: lessons from nature. Wiley, Hoboken

    Book  Google Scholar 

  • Gupta S, Banerjee S (2009) Food preference of goldfish (Carassius auratus (Linnaeus, 1758)) and its potential in mosquito control. J Ichth 2:47–58

    Google Scholar 

  • Houng H-S H, Chung-Ming Chen R, Vaughn DW, Kanesa-thasan N (2001) Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1–4 using conserved and serotype-specific 3’ noncoding sequences. J Virol Methods 95:19–32

    Article  PubMed  Google Scholar 

  • Hu RL, Li SR, Kong FJ, Hou RJ, Guan XL, Guo F (2014) Inhibition effect of silver nanoparticles on herpes simplex virus 2. Gen Mol Res 13:7022–7028

    Article  CAS  Google Scholar 

  • Jain J, Arora S, Rajwade JM, Omray P, Khandelwal S, Paknikar KM (2009) Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Mol Pharm 6:1388–1401

    Article  CAS  PubMed  Google Scholar 

  • Jayasankar P (1998) Ornamental fish culture and trade: current status and prospects. Fish Chim 17:9–13

    Google Scholar 

  • Kalimuthu K, Lin SM, Tseng LC, Murugan K, Hwang J-S (2014) Bioefficacy potential of seaweed Gracilaria firma with copepod, Megacyclops formosanus for the control larvae of dengue vector Aedes aegypti. Hydrobiologia 741:113–123

    Article  CAS  Google Scholar 

  • Killeen GF, Fillinger U, Knols BG (2002) Advantages of larval control for African malaria vectors: low mobility and behavioural responsiveness of immature mosquito stages allow high effective coverage. Malaria J 1:8

    Article  Google Scholar 

  • Krishnamoorthy M, Sasikuma JM, Shamna R, Pandiarajan C, Sofia P, Nagarajan B (2011) Antioxidant activities of bark extract from mangroves, Bruguiera cylindrica (L.) Blume and Ceriops decandra Perr. Indian J Pharmacol 43:557–562

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Coll Surf B Biointerf 76:50–56

    Article  CAS  Google Scholar 

  • Lin CF, Wan SW, Cheng HJ, Lei HY, Lin YS (2006) Autoimmune pathogenesis in dengue virus infection. Viral Immunol 19:127–32

    Article  CAS  PubMed  Google Scholar 

  • Lu L, Sun RW, Chen R, Hui CK, Ho CM, Luk JM, Lau GK, Che CM (2008) Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther 13:253–262

    CAS  PubMed  Google Scholar 

  • Mahitha B, Deva Prasad Raju B, Dillip GR, Reddy CM, Mallikarjuna K, Manoj L, Priyanka S, Rao KJ, Sushma NJ (2011) Biosynthesis, characterization and antimicrobial studies of AgNPs extract from Bacopa monniera whole plant. Dig J Nanomater Bios 6:135–142

    Google Scholar 

  • Marimuthu S, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Jayaseelan C, Bagavan A, Zahir AA, Elango G, Kamaraj C (2011) Evaluation of green synthesized silver nanoparticles against parasites. Parasitol Res 10:2212–2224

    Google Scholar 

  • Mehlhorn H (2011) Nature helps. How plants and other organisms contribute to solve health problems. Parasitol Res Monographs, Springer, Berlin, pp 1–372

    Google Scholar 

  • Mehlhorn H, Schmahl G, Schmidt J (2005) Extract of the seeds of the plant Vitex agnus castus proven to be highly efficacious as a repellent against ticks, fleas, mosquitoes and biting flies. Parasitol Res 95:363–365

    Article  PubMed  Google Scholar 

  • Mehlhorn H, Al-Rasheid KAS, Al-Quraishy S, Abdel-Ghaffar F (2012) Research and increase of expertise in arachno-entomology are urgently needed. Parasitol Res 110:259–265

    Article  PubMed  Google Scholar 

  • Milam CD, Farris JL, Wilhide JD (2000) Evaluating mosquito control pesticides for effect on target and nontarget organisms. Arch Environ Con Toxicol 39:324–328

    Article  CAS  Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramfrez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotech 16:2346–2353

    Article  CAS  Google Scholar 

  • Mukherjee P, Roy M, Mandal BP, Dey GK, Mukherjee PK Ghatak J, Tyagi AK, Kale SP (2008) Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum. Nanotechn 19:075103. doi:10.1088/0957-4484/19/7/075103

    Article  CAS  Google Scholar 

  • Mukherjee M, Chattopadyay M, Datta SK, Biswas S (2000) Problems and prospects of aquarium fish trade in West Bengal. Fish Chim 20:90–93

    Google Scholar 

  • Murugan K, Benelli G, Ayyappan S, Dinesh D, Panneerselvam C, Nicoletti M, Hwang JS, Mahesh Kumar P, Subramaniam J, Suresh U (2015a) Toxicity of seaweed-synthesized silver nanoparticles against the filariasis vector Culex quinquefasciatus and its impact on predation efficiency of the cyclopoid crustacean Mesocyclops longisetus. Parasitol Res 114:2243–2253

    Article  PubMed  Google Scholar 

  • Murugan K, Benelli G, Panneerselvam C, Subramaniam J, Jeyalalitha T, Dinesh D, Nicoletti M, Hwang JS, Suresh U, Madhiyazhagan P (2015b) Cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod Mesocyclops aspericornis against malaria and dengue mosquitoes. Exp Parasitol 153:129–138

    Article  CAS  PubMed  Google Scholar 

  • Murugan K, Priyanka V, Dinesh D, Madhiyazhagan P, Panneerselvam C, Subramaniam J, Suresh U, Chandramohan B, Roni M, Nicoletti M, Alarfaj AA, Higuchi A, Munusamy MA, Khater HF, Messing RH, Benelli G (2015c) Enhanced predation by Asian bullfrog tadpoles, Hoplobatrachus tigerinus, against the dengue vector Aedes aegypti in an aquatic environment treated with mosquitocidal nanoparticles. Parasitol Res. doi:10.1007/s00436-015-4582-0

    Google Scholar 

  • Murugan K, Venus JSE, Panneerselvam C, Bedini S, Conti B, Nicoletti M, Kumar Sarkar S, Hwang JS, Subramaniam J, Madhiyazhagan P, Mahesh Kumar P, Dinesh D, Suresh U, Benelli G (2015d) Biosynthesis, mosquitocidal and antibacterial properties of Toddalia asiatica-synthesized silver nanoparticles: do they impact predation of guppy Poecilia reticulata against the filariasis mosquito Culex quinquefasciatus? Environ Sci Poll Res. doi:10.1007/s11356- 015-4920-x

    Google Scholar 

  • Pavela R (2015a) Essential oils for the development of eco-friendly mosquito larvicides: A review. Ind Crops Prod 76:174–187

    Article  CAS  Google Scholar 

  • Pavela R (2015b) Acute toxicity and synergistic and antagonistic effects of the aromatic compounds of some essential oils against Culex quinquefasciatus Say larvae. Parasitol Res. doi:10.1007/s00436-015-4614-9

    PubMed  Google Scholar 

  • Rogers J, Parkinson C, Choi Y, Speshock J, Hussain S (2008) A preliminary assessment of silver nanoparticle inhibition of monkey pox virus plaque formation. Nanoscale Res Lett 3:129–133

    Article  PubMed Central  Google Scholar 

  • Roni M, Murugan K, Panneerselvam C, Subramaniam J, Hwang JS (2013) Evaluation of leaf aqueous extract and synthesized silver nanoparticles using Nerium oleander against Anopheles stephensi (Diptera: Culicidae). Parasitol Res 112:981–990

    Article  PubMed  Google Scholar 

  • Roni M, Murugan K, Panneerselvam C, Subramaniam J, Nicoletti M, Madhiyazhagan P, Dinesh D, Suresh U, Khater HF, Wei H, Canale A, Alarfaj AA, Munusamy MA, Higuchi A, Benelli G (2015) Characterization and biotoxicity of Hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against Aedes aegypti and Plutella xylostella. Ecotoxicol Environ Saf. doi:10.1016/j.ecoenv.2015.07.005

  • Sathyavathi R, Balamurali Krishna M, Venugopal Rao S, Saritha R, Narayana Rao D (2010) Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:1–6

    Article  Google Scholar 

  • Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi AA (2007) Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Proc Biochem 42:919–923

    Article  CAS  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Coll Interf Sci 275:496–502

    Article  CAS  Google Scholar 

  • Shreshtha V, Abirami S, Mahalakshmi V (2013) Anticancer and antibacterial activity of silver nan oparticles biosynthesized by Penicillium spp and its synergistic effect with antibiotics. J Microbiol Biotech Res 3(3):54–71

    Google Scholar 

  • Singh AK, Ahmed SH (2005) Ornamental fish culture in Bihar: Prospects as a Cottage Industry. Fish Chim 25:9–18

    Google Scholar 

  • Singh BP, Hatton BJ, Singh B, Cowie AL, Kathuria A (2010) Influence of biochars on nitrous oxide emission and nitrogen leaching from two contrastings oils. J Environ Qual 39:1–12

    Article  Google Scholar 

  • Singh T (2005) Emerging trends in world ornamental fish trade. Infofish Int 24(3):15–18

    Google Scholar 

  • Sivapriyajothi S, Mahesh Kumar P, Kovendan K, Subramaniam J, Murugan K (2014) Larvicidal and pupicidal activity of synthesized silver nanoparticles using Leucas aspera leaf extract against mosquito vectors, aedes aegypti and Anopheles stephensi. J Entomol Acarol Res 46:1787

    Article  Google Scholar 

  • Semmler M, Abdel-Ghaffar F, Al-Rasheid KAS, Mehlhorn H (2009) Nature helps: from research to products against blood sucking arthropods. Parasitol Res 105:1483–1487

    Article  PubMed  Google Scholar 

  • Subramaniam J, Murugan K, Panneerselvam C, Kovendan K, Madhiyazhagan P, Mahesh Kumar P, Dinesh D, Chandramohan B, Suresh U, Nicoletti M, Higuchi A, Hwang JS, Kumar S, Alarfaj AA, Munusamy MA, Messing RH, Benelli G (2015) Eco-friendly control of malaria and arbovirus vectors using the mosquitofish Gambusia affinis and ultra-low dosages of Mimusops elengi-synthesized silver nanoparticles: towards an integrative approach? Environ Sci Poll Res doi:10.1007/s11356-015-5253-5

  • Sujitha V, Murugan K, Paulpandi M, Panneerselvam C, Suresh U, Roni M, Nicoletti M, Higuchi A, Madhiyazhagan P, Subramaniam J, Dinesh D, Vadivalagan C, Chandramohan B, Alarfaj AA, Munusamy MA, Barnard DR, Benelli G (2015) Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti. Parasitol Res. doi:10.1007/s00436-015-4556-2

    PubMed  Google Scholar 

  • Suresh U, Murugan K, Benelli G, Nicoletti M, Barnard DR, Panneerselvam C, Mahesh Kumar P, Subramaniam J, Dinesh D, Chandramohan B (2015) Tackling the growing threat of dengue: Phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector Aedes aegypti (Diptera: Culicidae). Parasitol Res 114:1551–1562

    Article  PubMed  Google Scholar 

  • Vivek R, Thangam R, Muthuchelian K, Gunasekaran P, Kaveri K, Kannan S (2012) Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochem 47:2405–2410

    Article  CAS  Google Scholar 

  • Walker K, Lynch M (2007) Contributions of Anopheles larval control to malaria suppression in tropical Africa: review of achievements and potential. Med Vet Entomol 21:2–21

    Article  CAS  PubMed  Google Scholar 

  • Wattanachai P, Tintanon B (1999) Resistance of Aedes aegypti to chemical compounds in aerosol insecticide products in different areas of Bangkok, Thailand. Commun Dis J 25:188–191

    Google Scholar 

  • WHO (2015) Dengue and severe dengue. Fact sheet N°117. World Health Organization, Geneva

    Google Scholar 

  • Yildirim O, Karacuha A (2007) A preliminary study on determination of Aphanius chantrei’s feeding behaviour on mosquito larvae. Acta Trop 102:172–175

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Department of Science and Technology (New Delhi, India), Project No. DST/SB/EMEQ-335/2013, for providing financial support. The funder had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflicts of interest

The authors declare no conflicts of interest. Heinz Mehlhorn and Giovanni Benelli are Editor in Chief and Editorial Board Member of Parasitology Research, respectively. This does not alter the authors’ adherence to all the Parasitology Research policies on sharing data and materials.

Compliance with ethical standards

All applicable international and national guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Author information

Authors and Affiliations

  1. Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    Kadarkarai Murugan, Devakumar Dinesh, Manickam Paulpandi, Jayapal Subramaniam, Pari Madhiyazhagan, Udaiyan Suresh, Palanisamy Mahesh Kumar, Jagathish Mohan & Rajapandian Rajaganesh

  2. Faculty of Science, Department of Biology, University of Tabuk, Tabuk, 71491, Saudi Arabia

    Abdulaziz Dakhellah Meqbel Althbyani

  3. School of Life Science and Technology, Shanxi University, Taiyuan, 030006, China

    Lan Wang

  4. Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, China

    Hui Wei

  5. Institute of Marine Biology, National Taiwan Ocean University, Keelung, 20224, Taiwan

    Kandasamy Kalimuthu

  6. Texas A&M University System/Agri Life Research and Extension Center, Lubbock, TX, 79403, USA

    Megha N. Parajulee

  7. Department of Parasitology, Heinrich Heine University, Düsseldorf, Germany

    Heinz Mehlhorn

  8. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

Authors
  1. Kadarkarai Murugan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Devakumar Dinesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Manickam Paulpandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abdulaziz Dakhellah Meqbel Althbyani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jayapal Subramaniam
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pari Madhiyazhagan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Udaiyan Suresh
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Palanisamy Mahesh Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jagathish Mohan
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Rajapandian Rajaganesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Hui Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Kandasamy Kalimuthu
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Megha N. Parajulee
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Heinz Mehlhorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Giovanni Benelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Giovanni Benelli.

Electronic supplementary material

Below is the link to the electronic supplementary material.

(MP4 9177 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Murugan, K., Dinesh, D., Paulpandi, M. et al. Nanoparticles in the fight against mosquito-borne diseases: bioactivity of Bruguiera cylindrica-synthesized nanoparticles against dengue virus DEN-2 (in vitro) and its mosquito vector Aedes aegypti (Diptera: Culicidae). Parasitol Res 114, 4349–4361 (2015). https://doi.org/10.1007/s00436-015-4676-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-015-4676-8

Keywords

Search

Navigation