Abstract
In the present study, the treatment of ichthyophthiriasis with medicated feed was investigated in rainbow trout, Oncorhynchus mykiss, and chub, Leuciscus cephalus. The anti-parasitics toltrazuril and imidocarb; the antibiotics doxycycline, erythromycin and sulphadiazine and the anti-inflammatory acetylsalicylic acid were tested. In vitro experiment revealed that all tested anti-parasitics and antibiotics were effective in killing the isolated trophonts and theronts. Minimum doses for killing 100 % of the viable trophonts and for inhibiting the development of theronts were 3 mg/L for doxycycline, 30 mg/L for erythromycin, 2 mg/L for imidocarb dipropionate, 30 mg/L for sulphadiazine and 20 mg/L for toltrazuril. Acetylsalicylic acid (40 mg/kg fish/day), doxycycline (3 and 6 mg/kg/day), erythromycin (40 mg/kg/day), imidocarb dipropionate (5.0 mg/kg/day), sulphadiazine (40 mg/kg/day), toltrazuril (20 and 40 mg/kg/day) and combinations of doxycycline and toltrazuril (3 + 20 mg/kg/day, 6 + 40 mg/kg/day) were tested as medicated feed. When administered as medicated feed, only doxycycline, toltrazuril and combinations of doxycycline and toltrazuril reduced the fish mortality and infestation level. Best results were obtained by feeding a combination of 6 mg/kg/day doxycycline and 40 mg/kg/day toltrazuril. In O. mykiss, this treatment reduced the mortality rate from 100 to 50 ± 14 % after 10 days and the infestation level from grade 4 (≥100 trophonts per skin mucus sample) to 3.5 (50–100 trophonts). In L. cephalus, the mortality rate was decreased from 100 to 39 ± 5 % and the infestation level from grades 4 to 2 (ten to 50 trophonts) after 10 days.
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References
Buchmann K, Jensen PB, Kruse KD (2003) Effects of sodium percarbonate and garlic extract on Ichthyophthirius multifiliis theronts and tomocysts: in vitro experiments. North Am J Aquaculture 65:21–24
Chapman GB (1984) Ultrastructural aspects of the host–parasite relationship in ichthyophthiriasis. Trans Am Microsc Soc 103:364–375
Coldham NG, Moore AS, Dave M, Graham PJ, Sivapathasundaram S, Lake BG, Sauer MJ (1995) Imidocarb residues in edible bovine tissues and in vitro assessment of imidocarb metabolism and cytotoxicity. Drug Metabol Disp 23:501–505
Dickerson HW (2006) Fish diseases and disorders. In: Woo PTK (ed) Protozoan and metazoan infections, vol 1. CABI International, Oxfordshire, pp 128–152
Ekanem AP, Obiekezie A, Kloas W, Knopf K (2004) Effects of crude extracts of Mucuna pruriens (Fabaceae) and Carica papaya (Caricaceae) against the protozoan fish parasite Ichthyophthirius multifiliis. Parasitol Res 92:361–366
Elmusharaf MA, Beynen AC (2007) Alternative anticoccidial treatment of broiler chickens. Ph.D. thesis, Utrecht University
Harder A, Haberkorn A (1989) Possible mode of action of toltrazuril: studies on two Eimeria species and mammalian and Ascaris suum enzymes. Parasitol Res 78:8–12
Heinecke RD, Buchmann K (2009) Control of Ichthyophthirius multifiliis using a combination of water filtration and sodium percarbonate: dose–response studies. Aquaculture 288:32–35
Hines RS, Spira DT (1974) Ichthyophthiriasis in the mirror carp Cyprinus carpio (L.) V. Acquired immunity. J Fish Biol 6:373–378
Höfler W (1981) Persisting symptomatic human coccidiosis (Isospora belli). Treatment with sulphadiazine and pyrimethamine. Trans Res Soc Trop Med Hyg 75:184–186
Kirby GC (1996) Medicinal plants and the control of protozoal disease with particular reference to malaria. Trans Res Soc Trop Med Hyg 90:605–609
Koppang EO, Fischer U, Satoh M, Jirillo E (2007) Inflammation in fish as seen from a morphological point of view with special reference to the vascular compartment. Curr Pharm Des 13:3649–3655
Lahnsteiner F, Weismann T (2007) Treatment of ichthyophthiriasis in rainbow trout and common carp with common and alternative therapeutics. J Aquat Anim Health 19:186–194
Lawrence SA (2004) Amines: synthesis, properties and applications. Cambridge University Press, London
Ling F, Lu C, Tu X, Yi Y, Huang A, Zhang Q, Wang G (2013) Antiprotozoal screening of traditional medicinal plants: evaluation of crude extract of Psoralea corylifolia against Ichthyophthirius multifiliis in goldfish. Parasitol Res 112:2331–2340
Luzardo-Alvarez A, Martinez-Mazagatos J, Santamarina-Fernandez MT, Otero-Espinar FJ, Blanco-Mendez J (2003) Oral pharmacological treatments for ichthyophthiriosis of rainbow trout (Oncorhynchus mykiss). Aquaculture 220:15–25
Maki JL, Brown CC, Dickerson HW (2001) Occurrence of Ichthyophthirius multifiliis within the peritoneal cavities of infected channel catfish Ictalurus punctatus. Dis Aquat Organ 26:41–45
Meyohas MC, Capella F, Poirot JL, Lecomte I, Binet D, Eliaszewicz M, Frottier J (1990) Treatment with doxycycline and nifuroxazide of Isospora belli infection in AIDS. Pathol Biol 38:589–591
Picón-Camacho SM, Marcos-Lopez M, Beljean A, Debeaume S, Shinn AP (2012) In vitro assessment of the chemotherapeutic action of a specific hydrogen peroxide, peracetic, acetic, and peroctanoic acid-based formulation against the free-living stages of Ichthyophthirius multifiliis (Ciliophora). Parasitol Res 110:1029–1032
Rach J, Gaikowski MP, Ramsay RT (2000) Efficacy of hydrogen peroxide to control parasitic infestations on hatchery-reared fish. J Aquat Anim Health 12:267–273
Riviere JE, Spoo JW (2001) Tetracycline antibiotics. In: Adams HR (ed) Veterinary pharmacology and therapeutics. Iowa State Press, Ames
Schlenk D, Gollon J, Griffin BR (1998) Efficacy of copper sulfate for the treatment of ichthyophthiriasis in channel catfish. J Aquat Anim Health 10:390–396
Shinn AP, Wootten R, Côté I, Sommerville C (2003) Efficacy of selected oral chemotherapeutants Ichthyophthirius multifiliis (Ciliophora: Ophyroglenidae) infecting rainbow trout Oncorhynchus mykiss. Dis Aquatic Org 55:17–22
Spoo JW, Riviere JE (2001) Sulfonamides. In: Adams HR (ed) Veterinary pharmacology and therapeutics. Iowa State Press, Ames
Stoskopf MK (1993) Fish medicine. Saunders, London
Straus DL, Griffin BR (2001) Prevention of an initial infestation of Ichthyophthirius multifiliis in channel catfish and blue tilapia by potassium permanganate treatment. North Am J Aquaculture 63:11–16
Sun HY, Noe J, Barber J, Coyne RS, Cassidy-Hanley D, Clark TG, Findly RC, Dickerson HW (2009) Endosymbiotic bacteria in the parasitic ciliate Ichthyophthirius multifiliis. App Environ Microbiol 23:7445–7452
Tojo J, Santamarina MT (2001) Attempts at oral pharmacological treatment of Ichthyophthirius multifiliis in rainbow trout Oncorhynchus mykiss. J Fish Dis 24:249–252
Valtonen ET, Keranen AL (1981) Ichthyophthiriasis of Atlantic salmon, Salmo salar L, at the Montta hatchery in Northern Finland in 1978–1979. J Fish Dis 4:405–411
Wohllebe S, Richter P, Häder DP (2012) Chlorophyllin for the control of Ichthyophthirius multifiliis (Fouquet). Parasitol Res 111:729–733
Xu DH, Phillip H, Klesius PH (2004) Two-year study on the infectivity of Ichthyophthirius multifiliis in channel catfish, Ictalurus punctatus. Dis Aquatic Org 59:131–134
Yao JY, Shen JY, Li XL, Xu Y, Hau GJ, Pan XY, Wang GX, Yin WL (2010) Effect of sanguinarine from the leaves of Macleaya cordata against Ichthyophthirius multifiliis in grass carp (Ctenopharyngodon idella). Parasitol Res 107:1035–1042
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Abdel-Hafez, G., Lahnsteiner, F. & Mansour, N. Possibilities to control Ichthyophthirius multifiliis infestation with medicated feed in rainbow trout (Oncorhynchus mykiss) and chub (Leuciscus cephalus). Parasitol Res 113, 1119–1126 (2014). https://doi.org/10.1007/s00436-013-3749-9
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DOI: https://doi.org/10.1007/s00436-013-3749-9