Abstract
Main conclusion
This study demonstrates that brassinosteroid is essential for seedling and shoot growth in moso bamboo.
Abstract
The shoot of moso bamboo is known to grow extremely fast. The roles of phytohormones in such fast growth of bamboo shoot remain unclear. Here we reported that endogenous brassinosteroid (BR) is a major factor promoting bamboo shoot internode elongation. Reducing endogenous brassinosteroid level by its biosynthesis inhibitor propiconazole stunted shoot growth in seedling stage, whereas exogenous BR application promoted scale leaf elongation and the inclination of lamina joint of leaves and scale leaves. Genome-wide transcriptome analysis identified hundreds of genes whose expression levels are altered by BR and propiconazole in shoots and roots of bamboo seedling. The data show that BR regulates cell wall-related genes, hydrogen peroxide catabolic genes, and auxin-related genes. Our study demonstrates an essential role of BR in fast growth bamboo shoots and identifies a large number of BR-responsive genes in bamboo seedlings.
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Data availability
The RNA-seq raw data were deposited in the National Center for Biotechnology Information database (GSE123529).
Abbreviations
- BR:
-
Brassinosteroid
- BL:
-
Brassinolide
- DEG:
-
Differentially expressed gene
- eBL:
-
2,4-Epicastasterone
- GO:
-
Gene ontology
- PPZ:
-
Propiconazole
- ROS:
-
Reactive oxygen species
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Acknowledgements
This work was supported by a grant from the National Natural Science Foundation of China Grant (No. 31700254) and FAFU-International Collaborative Program (KXb16005A) to WW, the National Natural Science Foundation of China Grant (No. 31500226) to XY, the National Natural Science Foundation of China Grant (No. 31600230) to KS, the National Natural Science Foundation of China Grant (No. 31971734) to LG, and the Program for scientific and technological innovation team in University of Fujian province (No. 118/KLA18069A) to QZ.
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Zhang, Z., Yang, X., Cheng, L. et al. Physiological and transcriptomic analyses of brassinosteroid function in moso bamboo (Phyllostachys edulis) seedlings. Planta 252, 27 (2020). https://doi.org/10.1007/s00425-020-03432-z
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DOI: https://doi.org/10.1007/s00425-020-03432-z