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The pepper extracellular peroxidase CaPO2 is required for salt, drought and oxidative stress tolerance as well as resistance to fungal pathogens

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Abstract

In plants, biotic and abiotic stresses regulate the expression and activity of various peroxidase isoforms. Capsicum annuum EXTRACELLULAR PEROXIDASE 2 (CaPO 2) was previously shown to play a role in local and systemic reactive oxygen species bursts and disease resistance during bacterial pathogen infection. Here, we report CaPO 2 expression patterns and functions during conditions of biotic and abiotic stress. In pepper plants, CaPO 2 expression was strongly induced by abscisic acid, but not by defense-related plant hormones such as salicylic acid, ethylene and jasmonic acid. CaPO 2 was also strongly induced by abiotic and biotic stress treatments, including drought, cold, high salinity and infection by the hemibiotrophic fungal pathogen Colletotrichum coccodes. Loss-of-function of CaPO 2 in virus-induced gene silenced pepper plants led to increased susceptibility to salt- and osmotic-induced stress. In contrast, CaPO 2 overexpression in transgenic Arabidopsis thaliana plants conferred enhanced tolerance to high salt, drought, and oxidative stress, while also enhancing resistance to infection by the necrotrophic fungal pathogen Alternaria brassicicola. Taken together, these results provide evidence for the involvement of pepper extracellular peroxidase CaPO2 in plant defense responses to various abiotic stresses and plant fungal pathogens.

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Abbreviations

CaPO 2 :

Capsicum annum extracellular peroxidase 2

VIGS:

Virus-induced gene silencing

TRV:

Tobacco rattle virus

OX:

Overexpression

ROS:

Reactive oxygen species

ABA:

Abscisic acid

MV:

Methyl viologen

TBARS:

Thiobarbituric acid reactive substance

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Acknowledgments

This work was supported by the Next Generation BioGreen21 Program (Plant Molecular Breeding Center; Grant No. PJ008027), Rural Development Administration, Republic of Korea.

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  1. Hyong Woo Choi

    Present address: Boyce Thompson Institute for Plant Research, Tower Road Ithaca, New York, 14853-1801, USA

Authors and Affiliations

  1. Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Hyong Woo Choi & Byung Kook Hwang

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  1. Hyong Woo Choi
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Correspondence to Byung Kook Hwang.

Additional information

The nucleotide sequence data reported here have been deposited in the GenBank database under the accession number DQ489711.

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Choi, H.W., Hwang, B.K. The pepper extracellular peroxidase CaPO2 is required for salt, drought and oxidative stress tolerance as well as resistance to fungal pathogens. Planta 235, 1369–1382 (2012). https://doi.org/10.1007/s00425-011-1580-z

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