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Abscisic acid and hydrogen peroxide induce a novel maize group C MAP kinase gene, ZmMPK7, which is responsible for the removal of reactive oxygen species

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Abstract

Mitogen-activated protein kinase (MAPK) cascades are involved in biotic and abiotic stress responses. In plants, MAPKs are classified into four groups, designated A–D. Information about group C MAPKs is limited, and, in particular, no data from maize are available. In this article, we isolated a novel group C MAPK gene, ZmMPK7, from Zea mays. Exogenous abscisic acid (ABA) and hydrogen peroxide (H2O2) induced calcium-dependant transcription of ZmMPK7. Induction of this gene in response to ABA was blocked by several reactive oxygen species (ROS) manipulators such as imidazole, Tiron, and dimethylthiourea (DMTU). This result indicates that endogenous H2O2 may be required for ZmMPK7-mediated ABA signaling. Expression of ZmMPK7 in Nicotonia tobaccum caused less H2O2 to accumulate and alleviated ROS-mediated injuries following submission of the plants to osmotic stress. The enhanced total peroxidase (POD) activity in transgenic tobacco plants may contribute to removal of ROS. Finally, we have shown that the ZmMPK7 protein localizes in the nucleus. These results broaden our knowledge regarding plant group C MAPK activity in response to stress signals.

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Abbreviations

ABA:

Abscisic acid

CaMV:

Cauliflower mosaic virus

DMTU:

Dimethylthiourea

DAB:

3,3′-Diaminobenzidine

GFP:

Green fluorescent protein

H2O2 :

Hydrogen peroxide

JA:

Jasmonic acid

MDA:

Malondialdehyde

PAGE:

Polyacrylamide gel electrophoresis

POD:

Peroxidase

ROS:

Reactive oxygen species

SA:

Salicylic acid

SOD:

Superoxide dismutase

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Acknowledgments

The authors gratefully acknowledge the help from Plant Developmental Biology Laboratory in Shandong Agricultural University, China. This work was supported by the Grants from the Nation Natural Science Foundation of China (No. 30871457, 30471052) and the Program for Changjiang Scholars and Innovative Research Teams in Universities (No. IRT0635).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People’s Republic of China

    Xiao-juan Zong, Da-peng Li, Ling-kun Gu, De-quan Li, Li-xia Liu & Xiao-li Hu

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  1. Xiao-juan Zong
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  2. Da-peng Li
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Correspondence to De-quan Li.

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Zong, Xj., Li, Dp., Gu, Lk. et al. Abscisic acid and hydrogen peroxide induce a novel maize group C MAP kinase gene, ZmMPK7, which is responsible for the removal of reactive oxygen species. Planta 229, 485–495 (2009). https://doi.org/10.1007/s00425-008-0848-4

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