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The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

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Abstract

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 was analyzed by an Agrobacterium-mediated transient expression assay in tobacco leaves. Several stress-related cis-acting elements (GT-1, W-box and ABRE) are located within the CaPIMP1 promoter. In tobacco leaf tissues transiently transformed with a CaPIMP1 promoter-β-glucuronidase (GUS) gene fusion, serially 5′-deleted CaPIMP1 promoters were differentially activated by Pseudomonas syringae pv. tabaci, ethylene, methyl jasmonate, abscisic acid, and nitric oxide. The −1,193 bp region of the CaPIMP1 gene promoter sequence exhibited full promoter activity. The −417- and −593 bp promoter regions were sufficient for GUS gene activation by ethylene and methyl jasmonate treatments, respectively. However, CaPIMP1 promoter sequences longer than −793 bp were required for promoter activation by abscisic acid and sodium nitroprusside treatments. CaPIMP1 expression was activated in pepper leaves by treatment with ethylene, methyl jasmonate, abscisic acid, β-amino-n-butyric acid, NaCl, mechanical wounding, and low temperature, but not with salicylic acid. Overexpression of CaPIMP1 in Arabidopsis conferred hypersensitivity to mannitol, NaCl, and ABA during seed germination but not during seedling development. In contrast, transgenic plants overexpressing CaPIMP1 exhibited enhanced tolerance to oxidative stress induced by methyl viologen during germination and early seedling stages. These results suggest that CaPIMP1 expression may alter responsiveness to environmental stress, as well as to pathogen infection.

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Abbreviations

ABA:

Abscisic acid

BABA:

β-Amino-n-butyric acid

ERE:

Ethylene-responsive element

GUS:

β-Glucuronidase

MeJA:

Methyl jasmonate

MV:

Methyl viologen

OX:

Overexpression

PR:

Pathogenesis-related

SA:

Salicylic acid

SNP:

Sodium nitroprusside

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Acknowledgments

This research was supported by a grant (CG1133) from the Crop Functional Genomics Center of the 21st Century, Frontier Research Program, funded by the Ministry of Science and Technology, Korea.

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Authors and Affiliations

  1. Department of Horticulture, College of Life Sciences and Natural Resources, Jinju National University, 150 Chilamdong, Jinju, Kyungnam, 660-758, Republic of Korea

    Jeum Kyu Hong

  2. Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Byung Kook Hwang

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  1. Jeum Kyu Hong
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Correspondence to Byung Kook Hwang.

Additional information

The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number DQ356279.

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Hong, J.K., Hwang, B.K. The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants. Planta 229, 249–259 (2009). https://doi.org/10.1007/s00425-008-0824-z

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