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Localisation of Arabidopsis NDPK2—revisited

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Abstract

Phytochromes are light responsive photoreceptors in plants that influence development and differentiation during the entire plant life cycle. Plant nucleoside diphosphate kinase 2 (NDPK2) has been reported to be a component of the light-mediated signalling cascade and to interact physically with phytochrome A in the cytosol. By using diverse methods as in vitro imports, in vivo localisation of GFP-fusion proteins and immuno blotting of plant cell fractions we clearly localise NDPK2 only to chloroplasts but not to the cytosol, demonstrating that although high affinity protein–protein interactions can occur in vitro, their physiological relevance can be artificial if the proteins are localised to different cell compartments in vivo.

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Abbreviations

NDPK:

Nucleoside diphosphate kinase

FNR:

Ferredoxin oxidoreductase

GFP:

Green fluorescent protein

RFP:

Red fluorescent protein

DAPI:

4′,6,-Diamidino-2-phenylindol

References

  • Amann K, Lezhneva L, Wanner G, Herrmann RG, Meurer J (2004) Accumulation of photosystem one1, a member of a novel gene family, is required for accumulation of [4Fe–4S] cluster-containing chloroplast complexes and antenna proteins. Plant Cell 16:3084–3097

    Article  PubMed  CAS  Google Scholar 

  • Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamid gels. Electrophoresis 8:93–99

    Article  CAS  Google Scholar 

  • Briat J, Lescure A, Mache R (1986) Transcription of the chloroplast DNA: a review. Biochimie 68:981–990

    Article  PubMed  CAS  Google Scholar 

  • Chi-Ham CL, Keaton M, Cannon GC, Heinhorst S (2002) The DNA-compacting protein DCP68 from soybean chloroplasts is ferredoxin:sulfite reductase and co-localizes with the organellar nucleoid. Plant Mol Biol 49:621–630

    Article  PubMed  CAS  Google Scholar 

  • Choi G, Yi H, Lee J, Kwon YK, Soh MS, Shin B, Luka Z, Hahn TR, Song PS (1999) Phytochrome signalling is mediated through nucleoside diphosphate kinase 2. Nature 401:610–613

    Article  PubMed  CAS  Google Scholar 

  • Choi G, Kim JI, Hong SW, Shin B, Choi G, Blakeslee JJ, Murphy AS, Seo YW, Kim K, Koh EJ, Song PS, Lee H (2005) A possible role for NDPK2 in the regulation of auxin-mediated responses for plant growth and development. Plant Cell Physiol 46:1246–1254

    Article  PubMed  CAS  Google Scholar 

  • Cline K, Werner-Washburne M, Andrews J, Keegstra K (1984) Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. Plant Physiol 75:675–678

    Article  PubMed  CAS  Google Scholar 

  • Dorion S, Matton DP, Rivoal J (2006) Characterization of a cytosolic nucleoside diphosphate kinase associated with cell division and growth in potato. Planta 224:108–124

    Article  PubMed  CAS  Google Scholar 

  • Emanuelsson O, Nielsen H, von Heijne G (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Prot Sci 8:978–984

    CAS  Google Scholar 

  • Escobar Galvis ML, Hakansson G, Alexciev K, Knorpp C (1999) Cloning and characterisation of a pea mitochondrial NDPK. Biochimie 81:1089–1096

    Article  PubMed  CAS  Google Scholar 

  • Huber SC, Huber JL (1996) Role and regulation of sucrose-phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47:431–444

    Article  PubMed  CAS  Google Scholar 

  • Im YJ, Kim JI, Shen Y, Na Y, Han YJ, Kim SH, Song PS, Eom SH (2004) Structural analysis of Arabidopsis thaliana nucleoside diphosphate kinase-2 for phytochrome-mediated light signaling. J Mol Biol 343:659–670

    Article  PubMed  CAS  Google Scholar 

  • Kantor JD, McCormick B, Steeg PS, Zetter BR (1993) Inhibition of cell motility after nm23 transfection of human and murine tumor cells. Cancer Res 53:1971–1973

    PubMed  CAS  Google Scholar 

  • Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K, Galbraith D, Bohnert HJ (2001) Gene expression profiles during the initial phase of salt stress in rice. Plant Cell 13:889–905

    Article  PubMed  CAS  Google Scholar 

  • Keim D, Hailat N, Melhem R, Zhu XX, Lascu I, Veron M, Strahler J, Hanash SM (1992) Proliferation-related expression of p19/nm23 nucleoside diphosphate kinase. J Clin Invest 89:919–924

    Article  PubMed  CAS  Google Scholar 

  • Laemmli U (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Lakso M, Steeg PS, Westphal H (1992) Embryonic expression of nm23 during mouse organogenesis. Cell Growth Differ 3:873–879

    PubMed  CAS  Google Scholar 

  • Lubeck J, Soll J (1995) Nucleoside diphosphate kinase from pea chloroplasts: purification, cDNA cloning and import into chloroplasts. Planta 196:668–673

    Article  PubMed  CAS  Google Scholar 

  • Moon H, Lee B, Choi G, Shin D, Prasad DT, Lee O, Kwak SS, Kim DH, Nam J, Bahk J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci USA 100:358–363

    Article  PubMed  CAS  Google Scholar 

  • Nato A, Mirshahi A, Tichtinsky G, Mirshahi M, Faure JP, Lavergne D, De Buyser J, Jean C, Ducreux G, Henry Y (1997) Immunological detection of potential signal-transduction proteins expressed during wheat somatic tissue culture. Plant Physiol 113:801–807

    Article  PubMed  CAS  Google Scholar 

  • Nomura T, Fukui T, Ichikawa A (1991) Purification and characterization of nucleoside diphosphate kinase from spinach leaves. Biochim Biophys Acta 1077:47–55

    PubMed  CAS  Google Scholar 

  • Nomura T, Yatsunami K, Honda A, Sugimoto Y, Fukui T, Zhang J, Yamamoto J, Ichikawa A (1992) The amino acid sequence of nucleoside diphosphate kinase I from spinach leaves, as deduced from the cDNA sequence. Arch Biochem Biophys 297:42–45

    Article  PubMed  CAS  Google Scholar 

  • Ogura Y, Yoshida Y, Ichimura K, Aoyagi C, Yabe N, Hasunuma K (1999) Isolation and characterization of Neurospora crassa nucleoside diphosphate kinase NDK-1. Eur J Biochem 266:709–714

    Article  PubMed  CAS  Google Scholar 

  • Okabe-Kado J, Kasukabe T, Honma Y, Hayashi M, Hozumi M (1988) Purification of a factor inhibiting differentiation from conditioned medium of nondifferentiating mouse myeloid leukemia cells. J Biol Chem 263:10994–10999

    PubMed  CAS  Google Scholar 

  • Ouatas T, Salerno M, Palmieri D, Steeg PS (2003) Basic and translational advances in cancer metastasis: Nm23. J Bioenerg Biomembr 35:73–79

    Article  PubMed  CAS  Google Scholar 

  • Parks RE, Agarwal RP (1973) Nucleoside diphosphokinases. Enzymes 8:307–337

    CAS  Google Scholar 

  • Peltier JB, Cai Y, Sun Q, Zabrouskov V, Giacomelli L, Rudella A, Ytterberg AJ, Rutschow H, van Wijk KJ (2006) The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts. Mol Cell Proteomics 5:114–133

    Article  PubMed  CAS  Google Scholar 

  • Postel EH, Berberich SJ, Flint SJ, Ferrone CA (1993) Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis. Science 261:478–480

    Article  PubMed  CAS  Google Scholar 

  • Sheen J (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol 127:1466–1475

    Article  PubMed  CAS  Google Scholar 

  • Spetea C, Hundal T, Lundin B, Heddad M, Adamska I, Andersson B (2004) Multiple evidence for nucleotide metabolism in the chloroplast thylakoid lumen. Proc Natl Acad Sci USA 101:1409–1414

    Article  PubMed  CAS  Google Scholar 

  • Sweetlove LJ, Mowday B, Hebestreit HF, Leaver CJ, Millar AH (2001) Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria. FEBS Lett 508:272–276

    Article  PubMed  CAS  Google Scholar 

  • Yang LM, Lamppa GK (1996) Rapid purification of a chloroplast nucleoside diphosphate kinase using CoA-affinity chromatography. Biochim Biophys Acta 1294:99–102

    PubMed  Google Scholar 

  • Yano A, Umeda M, Uchimiya H (1995) Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.). Plant Mol Biol 27:1053–1058

    Article  PubMed  CAS  Google Scholar 

  • Zimmermann S, Baumann A, Jaekel K, Marbach I, Engelberg D, Frohnmeyer H (1999) UV-responsive genes of Arabidopsis revealed by similarity to the Gcn4-mediated UV response in yeast. J Biol Chem 274:17017–17024

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We would like to thank Dr J. Meurer for providing the plasmid coding for APO1-GFP and Dr J. Schwenn (Ruhr-University, Bochum) for the antibody against SiR. This work was supported by funds from the Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Department of Botany, University of Munich, Menzinger Str. 67, 80638, Munich, Germany

    Bettina Bölter, Rita Sharma & Jürgen Soll

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  1. Bettina Bölter
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  2. Rita Sharma
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  3. Jürgen Soll
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Correspondence to Jürgen Soll.

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Bölter, B., Sharma, R. & Soll, J. Localisation of Arabidopsis NDPK2—revisited. Planta 226, 1059–1065 (2007). https://doi.org/10.1007/s00425-007-0549-4

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