Abstract
Huge advances achieved recently in elucidating the role of NO in plants have been made possible by the application of NO donors. However, the application of NO to plants in various forms and doses should be subjected to detailed verification criteria. Not all metabolic responses induced by NO donors are reliable and reproducible in other experimental designs. The aim of the presented studies was to investigate the half-life of the most frequently applied donors (SNP, SNAP and GSNO), the rate of NO release under the influence of light and reducing agents. At a comparable donor concentration (500 μM) and under light conditions the highest rate of NO generation was found for SNAP, followed by GSNO and SNP. The measured half-life of the donor in the solution was 3 h for SNAP, 7 h for GSNO and 12 h for SNP. A temporary lack of light inhibited NO release from SNP, both in the solution and SNP-treated leaf tissue, which was measured by the electrochemical method. Also a NO, selective fluorescence indicator DAF-2DA in leaves supplied with different donors showed green fluorescence spots in the epidermal cells mainly in the light. SNP as a NO donor was the most photosensitive. The activity of PAL, which plays an important role in plant defence, was also activated by SNP in the light, not in the dark. S-nitrosothiols (SNAP and GSNO) also underwent photodegradation, although to a lesser degree than SNP. Additionally, NO generation capacity from S-nitrosothiols was shown in the presence of reducing agents, i.e. ascorbic acid and GSH, and the absence of light. The authors of this paper would like to polemicize with the commonly cited statement that “donors are compounds that spontaneously break down to release NO” and wish to point out the fact that the process of donor decomposition depends on the numerous external factors. It may be additionally stimulated or inhibited by live plant tissue, thus it is necessary to take into consideration these aspects and monitor the amount of NO released by the donor.
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Abbreviations
- NO:
-
Nitric oxide
- SNP:
-
Sodium nitroprusside
- RSNOs:
-
S-nitrosothiols
- GSNO:
-
S-nitrosoglutathione
- SNAP:
-
S-nitroso-N-acetyl-d-penicillamine
- PAL:
-
Phenylalanine ammonia lyase
- GSH:
-
Reduced glutathione
- CPTIO:
-
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
- DAF- 2DA:
-
4,5-diaminofluorescein diacetate
References
Aleryani S, Milo E, Kostka P (1999) Formation of peroxynitrite during thiol-mediated reduction of sodium nitroprusside. Biochim Biophys Acta 1472:181–190
Beligni MV, Lamattina L (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210:215–221
Beligni MV, Lamattina L (2001) Nitric oxide in plants: the history is just beginning. Plant Cell Environ 24:267–278
Ciszewski A, Milczarek G (2003) Electrochemical detection of nitric oxide using polymer modified electrodes. Talanta 61:11–26
Conrath U, Amoroso G, Köhle H, Sűltemeyer DF (2004) Non-invasive online detection of nitric oxide from plants and some other organisms by mass spectrometry. Plant J 38:1015–1022
Delledonne M (2005) NO news is good news for plants. Curr Opin Plant Biol 8:390–396
Duke SO, Naylor AW (1974) Effect of light on phenylalanine ammonia-lyase activity in dark-grown Zea mays (L.) seedlings. Plant Sci Lett 2:289–293
Durner J, Wendehenne D, Klessig DF (1998) Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci USA 95:10328–10333
Guo P, Cao Y, Li Z, Zhao B (2004) Role of an endogenous nitric oxide burst in the resistance of wheat to stripe rust. Plant Cell Environ 27:473–477
Grossi L, D’Angelo S (2005) Sodium nitroprusside: mechanism of NO release mediated by sulfohydryl-containing molecules. J Med Chem 48:2622–2626
Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol 40:347–369
Hou Y, Guo Z, Li J, Wang PG (1996) Seleno compounds and glutathione peroxidase catalyzed decomposition of S-nitrosothiols. Biochem Res Commun 228:89–93
Hou YC, Janczuk A, Wang PG (1999) Current trends in the development of nitric oxide donors. Curr Pharmac Des 5:417–441
Ioannidis I, Batz M, Paul T, Korth H-G, Susmann R, de Groot H (1996) Enhanced release of nitric oxide causes increased cytotoxicity of S-nitroso-N-acetyl-dl-penicillamine and sodium nitroprusside under hypoxic conditions. Biochem J 318:789–795
Leshem YY, Wills RBH, Ku VVV (1998) Evidence for the function of the free radical gas—nitric oxide (NO.)—as an endogenous maturation and senescence regulating factor in higher plants. Plant Physiol Biochem 36:825–833
Liu L, Hausladen A, Zeng M, Que L, Heitman J, Stamler JS (2001) A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans. Nature 410:490–494
Murgia I, de Pinto MC, Delledonne M, Soave C, de Gara L (2004) Comparative effects of various nitric oxide donors on ferritin regulation, programmed cell death, and cell redox state in plant cells. J Plant Physiol 161:777–783
Modolo LV, Cunha FQ, Braga MR, Salgado I (2002) Nitric oxide synthase-mediated phytoalexin accumulation in soybean cotyledones in response to the Diaporthe phaseolorum f. sp. meridionalis elicitor. Plant Physiol 130:1288–1297
Neill SJ, Desikan R, Hancock JT (2003) Nitric oxide signaling in plants. New Phytol 159:11–35
de Pinto MC, Tommasi F, de Gara L (2002) Changes in the antioxidant systems as part of the signaling pathway responsible for the programmed cell death activated by nitric oxide and reactive oxygen species in tobacco bright-yellow 2 cells. Plant Physiol 130:698–708
Privat C, Lantoine F, Bedioui F, Millanovoye van BE, Devynck J, Devynck MA (1997) Nitric oxide production by endothelial cells: comparison of three methods of quantification. Life Sci 61:1193–1202
del Rio LA, Corpas FJ, Barroso JB (2004) Nitric oxide and nitric oxide synthase activity in plants. Phytochemistry 65:783–792
Romero-Puertas MC, Perazzolli M, Zago E D, Delledonne M (2004) Nitric oxide signalling functions in plant-pathogen interactions. Cell Microbiol 6:795–803
Smith JN, Dasgupta TP (2000) Kinetics and mechanism of the decomposition of S-nitrosoglutathione by l-ascorbic acid and copper ions in aqueous solution to produce nitric oxide. Nitric Oxide 4:57–66
Seabra AB, de Souza GFP, da Rocha LL, Eberlin MN, de Oliveira MG (2004) S-nitrosoglutathione incorporated in poly (ethylene glycol) matrix: potential use for topical nitric oxide delivery. Nitric Oxide 11:263–272
Smith JN, Dasgupta TP (2001) Mechanisms of nitric oxide release from nitrovasodilators in aqueous solution: reaction of the nitroprusside ion ([Fe(CN)5NO]2−) with l-ascorbic acid. J Inorg Biochem 87:165–173
Stamler JS, Toone EJ (2002) The composition of thionitrite. Curr Opin Chem Biol 6:779–785
Trujillo M, Alvares MN, Pellufo G, Freeman BA, Radi R (1998) Xanthine oxidase-mediated decomposition of S-nitrosothiols. J Biol Chem 273:7828–7834
Wandehenne D, Durner J, Klessig DF (2004) Nitric oxide: a new player in plant signalling and defence. Curr Opin Plant Biol 7:449–455
Wang PG, Xian M, Tang X, Wu X, Wen Z, Cai T, Janczuk AJ (2002) Nitric oxide donors: chemical activities and biological applications. Chem Rev 102:1091–1134
Wang PG, Cai TB, Taniguchi N (2005) Nitric oxide donors for pharmaceutical and biological applications. Wiley-VCH, Weinheim
Ward EWB, Cahill DM, Bhattacharyya MK (1989) Abscisic acid suppression of phenylalanine ammonia-lyase activity and mRNA, and resistance of soybeans to Phytophthora megasperma f. sp. glycinea. Plant Physiol 91:23–27
Wojtaszek P (2000) Nitric oxide in plants to NO or not to NO. Phytochemistry 54:1–4
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This study was supported by the State Committee for Scientific Research (grant 2 P06A 016 27).
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Floryszak-Wieczorek, J., Milczarek, G., Arasimowicz, M. et al. Do nitric oxide donors mimic endogenous NO-related response in plants?. Planta 224, 1363–1372 (2006). https://doi.org/10.1007/s00425-006-0321-1
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DOI: https://doi.org/10.1007/s00425-006-0321-1