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Expression of PsGRP1, a novel glycine rich protein gene of Pisum sativum, is induced in developing fruit and seed and by ABA in pistil and root

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Abstract

A novel glycine-rich protein gene, PsGRP1, has been identified in Pisum sativum L. Accumulation of PsGRP1 transcripts was observed in reproductive organs and vegetative tissues. They were localized in endocarp sclerenchyma during fruit development in cells that will lignify. PsGRP1 expression was also detected in senescent pistils and developing seeds and induced by ABA treatment in presenescent pistils. A raise in the expression was also observed in roots after treatment with ABA or mannitol but not under cold stress. A mannitol treatment induced a rise in ABA levels and fluridone treatment counteracted the mannitol induction of PsGRP1 expression. The results suggest a possible role for PsGRP1 in differentiation of the endocarp sclerenchyma and during seed development, pistil senescence and osmotic stress under ABA control.

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Abbreviations

GRP:

Glycine-rich protein

ABA:

Abscisic acid

GA3 :

Gibberellic acid

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Acknowledgements

The authors wish to thank Dr. J. Rose (Cornell University), Dr. D. Alabadí, and Dr. I. Zarra for critical reading of the manuscript, Ms M.A. Argomániz for her excellent technical assistance, Dr. M.D. Gómez for help in the in-situ hybridization experiments, and Dr. T. Lafuente for help in ABA determinations. Financial support was provided by grants BIO2002-0483-C03-02 (MCyT, Spain) and BIO4 CT960621 (EU).

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  1. Manuel Cercós

    Present address: Instituto Valenciano de Investigaciones Agrarias, Centro de Genómica, Carretera Moncada - Náquera, km 4.6, 46113, Moncada, Spain

Authors and Affiliations

  1. Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia and Consejo Superior de Investigaciones Cientificas, Avenida de los Naranjos s/n, 46022, Valencia, Spain

    Cristina Urbez, Manuel Cercós, Miguel A. Perez-Amador & Juan Carbonell

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  1. Cristina Urbez
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Correspondence to Juan Carbonell.

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Urbez, C., Cercós, M., Perez-Amador, M.A. et al. Expression of PsGRP1, a novel glycine rich protein gene of Pisum sativum, is induced in developing fruit and seed and by ABA in pistil and root. Planta 223, 1292–1302 (2006). https://doi.org/10.1007/s00425-005-0178-8

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  • DOI: https://doi.org/10.1007/s00425-005-0178-8

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