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Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding

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Abstract

We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Δ166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Δ166 predominantly phosphorylates AtMEK1, an Arabidopsis MAPKK, but not its double mutant (AtMEK1T218A/S224E), suggesting that Thr-218 and Ser-224 are the phosphorylation sites. In wounded seedlings, AtMEKK1 was activated and phosphorylated its downstream AtMEK1. Furthermore, analysis using anti-AtMEKK1 and anti-AtMEK1 antibodies revealed that the interaction between the two proteins was signal dependent. These results suggest the presence of AtMEKK1–AtMEK1 pathway induced by wounding.

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Abbreviations

ATA:

Aurin tricarboxylic acid

CBB:

Coomasive Brilliant Blue

GST:

Glutathione-S-transferase

MAPK:

Mitogen-activated protein kinase

MBP:

Myelin basic protein

RT-PCR:

Reverse transcript polymerase chain reaction

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Acknowledgements

We are grateful to Dr. Ushio Kikkawa (Biosignal Research Center, Kobe University) for his encouragement and discussion.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Kobe University, Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Toto Hadiarto, Takashi Nanmori, Yasuo Fukami, Tetsushi Azuma & Takeshi Yasuda

  2. Research Center for Environmental Genomics, Kobe University, Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Takashi Nanmori, Tetsushi Iwasaki, Ken-ichi Sato & Yasuo Fukami

  3. Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka, 599-8570, Japan

    Daisuke Matsuoka

Authors
  1. Toto Hadiarto
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  2. Takashi Nanmori
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  3. Daisuke Matsuoka
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  4. Tetsushi Iwasaki
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  5. Ken-ichi Sato
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  6. Yasuo Fukami
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  7. Tetsushi Azuma
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  8. Takeshi Yasuda
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Correspondence to Takashi Nanmori.

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Hadiarto, T., Nanmori, T., Matsuoka, D. et al. Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding. Planta 223, 708–713 (2006). https://doi.org/10.1007/s00425-005-0126-7

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  • DOI: https://doi.org/10.1007/s00425-005-0126-7

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