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Cyclic electron flow around PSI monitored by afterglow luminescence in leaves of maize inbred lines (Zea mays L.): correlation with chilling tolerance

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Abstract

Maize (Zea mays L.) inbred lines of contrasting chilling sensitivity (three tolerant, three sensitive lines) were acclimated to 280 μmol photons m−2 s−1 white light at a 17°C sub-optimal temperature. They showed no symptoms of photoinhibition, despite slight changes in photosystem II (PSII) fluorescence and thermoluminescence properties in two tolerant lines. A luminescence “afterglow” emission [Bertsch and Azzi (1965) Biochim Biophys Acta 94:15–26], inducible by a far-red (FR) illumination of unfrozen leaf discs, was detected either as a bounce in decay kinetics at constant temperatures or as a sharp thermoluminescence afterglow band at about 45°C, in dark-adapted leaves. This band reflects the induction by warming of an electron pathway from stromal reductants to plastoquinones and to the QB secondary acceptor of PSII, resulting in a luminescence-emitting charge recombination in the fraction of centres that were initially in the S2/3QB non-luminescent state. A 5-h exposure of plants to growth chamber light shifted this luminescence emission towards shorter times and lower temperatures for several hours in the three chilling-tolerant lines. This downshift was not observed, or only transiently, in the three sensitive lines. In darkness, the downshifted afterglow band relaxed within hours to resume its dark-adapted location, similar for all maize lines. A faster dark re-reduction of P700+ oxidized by FR light (monitored by 820-nm absorbance) and an increase of photochemical energy storage under FR excitation (determined by photoacoustic spectroscopy) confirmed that a cyclic pathway induced by white actinic light remained activated for several hours in the tolerant maize lines.

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Abbreviations

DCMU:

3-(3′,4′-Dichlorophenyl)-1,1-dimethylurea (diuron), an inhibitor of photosystem II that binds to the QB pocket

E a :

Apparent activation energy (enthalpy) of charge recombination

1F, 2F:

1 or 2 flashes

FQR:

Ferredoxine-plastoquinone-reductase

F0, FV, FM:

Basal, variable and maximal chlorophyll fluorescence levels

PSI:

Photosystem I

PSII:

Photosystem II

QB:

Secondary quinonic acceptor of photosystem II

NDH:

NAD(P)H dehydrogenase (plastoquinone reductase)

R, S:

Chilling-tolerant and sensitive maize lines

TL:

Thermoluminescence

T m :

Temperature maximum of a thermoluminescence band

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Acknowledgements

This work was supported by a ACC-SV grant from the Ministry of Research and Technology and by the Comité Technique Permanent de la Sélection (CTPS). The Mumm-Perrier-Jouët company provided initial support to investigate spring chilling in grapevine. Seeds of the 1872 and 1192 inbred lines were kindly given by Euralis. We thank Dr. Sridharan Govindachary for the critical review of the manuscript.

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Authors and Affiliations

  1. Service de Bioénergétique, INRA/CEA-Saclay, 91191, Gif-sur-Yvette cedex, France

    Jean-Marc Ducruet & Miruna Roman

  2. Lasers Department, INFLPR, POB MG-36, Bucuresti-Magurele, Romania

    Miruna Roman

  3. Laboratoire d’Ecophysiologie de la Photosynthèse, CEA-Cadarache, DSV, DEVM, UMR 6191 CNRS-CEA-Aix-Marseille II, 13108, St Paul-lez-Durance, France

    Michel Havaux

  4. Agricultural Research Institute of the Hungarian Academy of Sciences, 2462, Martonvasar, POB 19, Hungary

    Tibor Janda

  5. INRA/INAPG/U-Paris-Sud: Génétique Végétale, ferme du Moulon, 91190, Gif-sur-Yvette, France

    André Gallais

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  1. Jean-Marc Ducruet
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  2. Miruna Roman
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  3. Michel Havaux
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  4. Tibor Janda
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Correspondence to Jean-Marc Ducruet.

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Ducruet, JM., Roman, M., Havaux, M. et al. Cyclic electron flow around PSI monitored by afterglow luminescence in leaves of maize inbred lines (Zea mays L.): correlation with chilling tolerance. Planta 221, 567–579 (2005). https://doi.org/10.1007/s00425-004-1464-6

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