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Cellular re-distribution of flavin-containing polyamine oxidase in differentiating root and mesocotyl of Zea mays L. seedlings

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Abstract

Plant polyamine oxidases (PAOs; EC 1.5.3.11) are hydrogen peroxide-producing enzymes supposedly involved in cell-wall differentiation processes and defence responses. Maize (Zea mays L.) PAO (MPAO) is a 53 kDa secretory glycoprotein, abundant in primary and secondary cell walls of several tissues. Using biochemical, histochemical, ultrastructural and immunocytochemical techniques, the distribution and sub-cellular compartmentalisation of MPAO in the primary root and mesocotyl of seedlings at different maturation stages or after growth under varying light conditions were analysed. In apical root tissues, MPAO immunoreactivity was mainly detected in the cytoplasmic compartment, while a lower immunoreactivity was observed in the cell walls. In the more mature, basal part of the root, intense immunogold labelling was found in the primary and secondary walls of protoxylem precursors and vessels, while endodermal cells and living metaxylem precursors were immunopositive both in their walls and in their thin cytoplasmic compartments. A re-distribution of MPAO protein from the cytoplasm toward the primary and secondary walls was also recognised when immunoreactivity of basal root tissues from 3-day-old seedlings was compared with that detected in 11-day-old tissues. Accordingly, biochemical analyses revealed MPAO entrapment in the extracellular matrix of mature tissues. In the mesocotyl, an enrichment of MPAO immunolabelling in the cell wall of protoxylem, metaxylem and epidermal tissues, as a function of light exposure, was observed. Taken together, these data support the hypothesised role of PAOs in cell-wall maturation. Moreover, the relevant intraprotoplasmic MPAO localisation observed mainly in differentiating root tissues suggests an additional role in intracellular production of hydrogen peroxide.

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Abbreviations

DAB:

3,3′-Diaminobenzidine

DPI:

Diphenyleneiodonium

MPAO:

Maize polyamine oxidase

PAO:

Polyamine oxidase

PCD:

Programmed cell death

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Acknowledgements

This work was supported by the Italian Ministry of University and Research (PRIN 2002). We thank Prof. Annarosa Luzzatto (Department of Biology, University “Roma Tre”, Italy) and Prof. Paola Bonfante (Plant Biology Department, Turin University, Italy) for helpful suggestions. We gratefully acknowledge Dr. Roberta Nardacci (IRCCS “L. Spallanzani”, Rome, Italy) for expert technical assistance.

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Authors and Affiliations

  1. Dipartimento di Biologia-LIME, Università “Roma Tre”, Viale Guglielmo Marconi 446, Rome, 00146, Italy

    Alessandra Cona, Sandra Moreno, Francesco Cenci, Rodolfo Federico & Riccardo Angelini

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  1. Alessandra Cona
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  2. Sandra Moreno
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  3. Francesco Cenci
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  4. Rodolfo Federico
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  5. Riccardo Angelini
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Correspondence to Riccardo Angelini.

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Alessandra Cona and Sandra Moreno have contributed equally to this paper.

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Cona, A., Moreno, S., Cenci, F. et al. Cellular re-distribution of flavin-containing polyamine oxidase in differentiating root and mesocotyl of Zea mays L. seedlings. Planta 221, 265–276 (2005). https://doi.org/10.1007/s00425-004-1435-y

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