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Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants

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Abstract

Immediate early responsive genes were screened by the differential display method during the hypersensitive response upon tobacco mosaic virus infection of tobacco (Nicotiana tabacum L.) plants carrying the N gene. Three hours after temperature shift from 30 °C to 20 °C, an increase in transcripts of a particular clone was observed. The cDNA encoded a polypeptide of 330 amino acids, whose topology indicated it to be a seven-transmembrane protein, designated as Nt7TM1. This was confirmed by direct observation of cultured tobacco cells expressing an Nt7TM1–green fluorescent protein fusion protein, which migrated exclusively to the plasma membrane and the endoplasmic reticulum. RNA blot hybridization analysis indicated that Nt7TM1 transcripts were not induced by salicylic or jasmonic acids, ethylene or hydrogen peroxide. The results suggested the presence of a unique system for pathogen response involving a novel seven-transmembrane protein.

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Fig. 1.
Fig. 2a, b.
Fig. 3a–h.
Fig. 4a–e.

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Abbreviations

GFP:

green fluorescent protein

HR:

hypersensitive response

MeJA:

jasmonic acid methylester

SA:

salicylic acid

7TM:

seven-transmembrane

TMV:

tobacco mosaic virus

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Acknowledgements

The authors thank Drs. N.-H. Chua (Rockefeller University New York, USA) and P. Spielhofer (University of Bern, Switzerland) for the generous gift of the pGFP-2 plasmid, and T. Nakagawa (Shimane University, Japan) for the pSA702 plasmid. The authors also thank Dr. K. Hiratsuka (Yokohama National University, Japan) for valuable discussion on GFP experiments, Ms. C. Ueda (Nara Institute of Science and Technology, Japan) for technical assistance, and Dr. M. Moore (Intermal, Nagoya, Japan) for critical reading of the manuscript. This work was supported by a grant for Research for the Future Program (JSPS-RFTF 00L01604) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan

    Yube Yamaguchi, Hiroshi Yoda, Kenji Akiyama & Hiroshi Sano

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  1. Yube Yamaguchi
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  2. Hiroshi Yoda
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  3. Kenji Akiyama
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  4. Hiroshi Sano
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Corresponding author

Correspondence to Hiroshi Sano.

Additional information

The nucleotide sequence reported in this article has been submitted to DDBJ under accession number AB024511

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Yamaguchi, Y., Yoda, H., Akiyama, K. et al. Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants. Planta 218, 249–253 (2003). https://doi.org/10.1007/s00425-003-1108-2

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  • DOI: https://doi.org/10.1007/s00425-003-1108-2

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