Abstract
Regenerating muscle fibers emerge from quiescent satellite cells, which differentiate into mature multinuclear myofibers upon activation. It has recently been found that ATOH8, a bHLH transcription factor, is regulated during myogenic differentiation. In this study, expression and localization of ATOH8, the other well-described regeneration markers, vimentin, nestin and neonatal myosin, and the satellite cell marker Pax7 were analyzed on protein level in human myopathy samples by immunofluorescence studies. On mRNA level, expression levels of ATOH8 and vimentin were studied by quantitative real-time PCR. ATOH8 is expressed in activated satellite cells and proliferating myoblasts of human skeletal muscle tissue. Quantitative analyses of ATOH8+, Pax7+, vimentin+, nestin+ and neonatal myosin+ muscle fibers showed the highest amount of regenerating muscle fibers in inflammatory myopathies, followed by muscular dystrophy. The relative co-expression of ATOH8 with the above-mentioned markers did not vary among the disorders. These results show that the novel regeneration marker ATOH8 contributes to muscle cell differentiation in healthy and diseased human muscle tissue.
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Abbreviations
- DAPI:
-
4′-6-Diamidino-2-phenylindole
- DM:
-
Dermatomyositis
- dsRNA:
-
Double-stranded ribonucleic acid
- LGMD:
-
Limb-girdle muscular dystrophy
- MD:
-
Muscular dystrophy
- MV:
-
Mean value
- PCR :
-
Polymerase chain reaction
- PM:
-
Polymyositis
- SEM:
-
Standard error of the mean
- IBM:
-
Inclusion body myositis
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Acknowledgments
Beate Brand-Saberi was supported by the MYORES Project (511978) funded by the EU’s Sixth Framework Program, FoRUM F647-09 and F732N-2011 of the Faculty of Medicine, Ruhr University Bochum and MERCUR PR 2012-0058. Anne-K. Güttsches was supported by FoRUM K069-12 of the Faculty of Medicine, Ruhr University Bochum. We sincerely thank J. Mertens-Rill and A. Schreiner for excellent technical support.
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Güttsches, AK., Balakrishnan-Renuka, A., Kley, R.A. et al. ATOH8: a novel marker in human muscle fiber regeneration. Histochem Cell Biol 143, 443–452 (2015). https://doi.org/10.1007/s00418-014-1299-6
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DOI: https://doi.org/10.1007/s00418-014-1299-6