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Hyalocyte proliferation and ECM accumulation modulated by bFGF and TGF-β1

  • Basic Science
  • Published:
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Abstract

Purpose

In cases of severe retinal diseases, the vitreous body has to be removed and replaced by a suitable biomaterial. Currently, however, no satisfying long-term vitreous substitute is in clinical use. A novel therapeutic concept represents the combination of hyalocytes with suitable biomaterials. The goal of the present study was to evaluate the potential of bFGF and TGF-β1 as tools to control hyalocyte proliferation and the accumulation of extracellular matrix (ECM).

Methods

In vitro investigation on the influence of different concentrations of bFGF and TGF-β1 on hyalocyte morphology, proliferation and ECM production.

Results

Both growth factors affected hyalocyte morphology; small, round cells could be observed after bFGF supplementation, whereas the cells appeared more completely spread when cultured with TGF-β1. Hyalocyte proliferation was increased 3-fold by 10 ng/ml bFGF; 1 ng/ml TGF-β1 in contrast reduced cell proliferation to about 40% of the control. Converse effects of the growth factors could also be observed on the ECM accumulation of hyalocytes; whereas bFGF halved ECM accumulation, TGF-β1 enhanced the ECM production up to 3-fold. Precultivation of hyalocytes with bFGF for two passages had no influence on their subsequent accumulation of glycosaminoglycans (GAG). However, cells precultivated with bFGF exhibited a doubled accumulation of collagen compared to controls.

Conclusions

The observed opposite effects of bFGF and TGF-β1 on hyalocyte proliferation and ECM accumulation may allow for the control of hyaloycte properties. Therefore, these two growth factors seem to be valuable tools towards the development of a cell-based vitreous substitute.

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Acknowledgement

This work was financially supported by grant 616/04 from the “Bayerische Forschungsstiftung”, Bavaria, Germany.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Institute of Pharmacy, University of Regensburg, Universitätsstraße 31, 93040, Regensburg, Germany

    Florian Sommer, Klaus Pollinger, Ferdinand Brandl, Barbara Weiser, Jörg Teßmar, Torsten Blunk & Achim Göpferich

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  1. Florian Sommer
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  2. Klaus Pollinger
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Correspondence to Achim Göpferich.

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The authors have full control of all primary data and they agree to allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review their data upon request.

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Sommer, F., Pollinger, K., Brandl, F. et al. Hyalocyte proliferation and ECM accumulation modulated by bFGF and TGF-β1. Graefes Arch Clin Exp Ophthalmol 246, 1275–1284 (2008). https://doi.org/10.1007/s00417-008-0846-z

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  • DOI: https://doi.org/10.1007/s00417-008-0846-z

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