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Headspace measurements of irradiated in vitro cultured cells using PTR-MS

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Abstract

A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4 Gy and 8 Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation.

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Acknowledgments

We would like to thank Klaudia Winkler and Romy Müller from the Institute of Radiation Biology of the Helmholtz Zentrum München, for providing the cells, the laboratory and the know-how for handling of in vitro cultured cells. We would also like to thank Lothar Keck, Bhushan Thekedar and Florian Wagner from the Institute of Radiation Protection for plenty of assistance with the development of the experimental setup and the data analysis.

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Authors and Affiliations

  1. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    C. Brunner, W. Szymczak, W. Li, C. Hoeschen & U. Oeh

  2. Institute of Radiology, Hospital rechts der Isar of the Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany

    C. Brunner

  3. Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    S. Mörtl & F. Eckardt-Schupp

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  1. C. Brunner
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  2. W. Szymczak
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  3. W. Li
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  4. C. Hoeschen
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  5. S. Mörtl
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  6. F. Eckardt-Schupp
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  7. U. Oeh
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Correspondence to C. Brunner.

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Brunner, C., Szymczak, W., Li, W. et al. Headspace measurements of irradiated in vitro cultured cells using PTR-MS. Radiat Environ Biophys 50, 209–217 (2011). https://doi.org/10.1007/s00411-010-0327-8

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  • DOI: https://doi.org/10.1007/s00411-010-0327-8

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