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Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation

  • Laryngology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate the myofiber subtype transition of human posterior cricoarytenoid (PCA) muscle after the injury to recurrent laryngeal nerve (RLN). PCA muscle specimens were obtained from 38 bilateral vocal fold paralysis patients underwent arytenoidectomy. According to the duration of RLN injury, all the cases were divided into five denervation groups: 6–12 months, 1–2, 2–3, 3–6, and >6 years. The normal PCA muscles from total laryngectomy patients were chosen as controls. Immunofluorescence was adopted to detect the expression level of myosin heavy chain (MHC)-I and MHC-II in PCA muscle. Quantitative real-time PCR was also used to assess the transcriptional level of MHC subtypes (MHC-I, MHC-IIa, MHC-IIb, MHC-IIx, embryonic-MHC, and peri-natal-MHC). Immunofluorescence showed that MHC-I-positive myofibers in denervation groups were much lower than control group, respectively, while MHC-II-positive myofibers were significantly higher than control group (P < 0.05). With the extension of denervation, the number of MHC-I-positive myofibers gradually decreased, while MHC-II gradually increased and peaked in 1- to 2-year group. Transcriptional level of MHC-I, MHC-IIa, and MHC-IIb in denervation groups significantly down-regulated compared with the control (P < 0.05), respectively. However, MHC-IIx, embryonic-MHC, and peri-natal-MHC significantly up-regulated in all denervation groups, and the highest level was in 1- to 2-year denervation group. Data from the present study demonstrated that the maximum transition of MHC subtypes in human PCA muscles occurred in 1–2 years after denervation, suggesting that laryngeal reinnervation before the occurrence of irreversible transition of MHC subtypes could maintain the structural integrity of laryngeal PCA muscles.

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Acknowledgments

This work was supported by Grant No. 81070774, 81371081, and 81400455 for science research from National Natural Science Foundation of China, and Grant No. 2010235 from Shanghai Municipal Commission of Health and Family Planning.

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    Authors and Affiliations

    1. Department of Otolaryngology-Head and Neck Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, 200433, China

      Xiaoxia Qiu, Donghui Chen, Meng Li, Yingna Gao, Fei Liu, Hongliang Zheng & Shicai Chen

    2. Department of Otolaryngology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

      Xiaoxia Qiu

    3. Otolaryngology-Head and Neck Surgery Center of Chinese PLA, Shanghai, 200433, China

      Shicai Chen

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    1. Xiaoxia Qiu
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    2. Donghui Chen
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    3. Meng Li
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    4. Yingna Gao
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    5. Fei Liu
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    6. Hongliang Zheng
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    Corresponding authors

    Correspondence to Hongliang Zheng or Shicai Chen.

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    X. Qiu, D. Chen and M. Li contributed equally to this research.

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    Qiu, X., Chen, D., Li, M. et al. Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation. Eur Arch Otorhinolaryngol 272, 2915–2923 (2015). https://doi.org/10.1007/s00405-015-3664-z

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    • DOI: https://doi.org/10.1007/s00405-015-3664-z

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