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Immunomodulation of tissue-engineered transplants: in vivo bone generation from methylprednisolone-stimulated chondrocytes

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Abstract

Subcutaneously implanted, in vitro engineered tissue is generally affected by the immune system of the host even in autogenous transplantation. The aim of this study was to investigate immunomodulation of subcutaneously implanted tissue-engineered cartilage transplants by intramuscular methylprednisolone application. Transplants consisted of auricular rabbit chondrocytes, polylactide-polyglycolide co-polymer fleeces and species-specific fibrin or agarose. The transplants were subcutaneously implanted in the ridge. Thereafter, animals were separated into two groups, one with and one without methylprednisolone treatment. The specimens were histologically investigated after 6 and 12 weeks. Fleece fiber degradation was complete after 12 weeks, and all transplants showed areas of calcification. The corticosteroid-treated group presented pronounced trabecular bone generation without fibrous tissue infiltration. The untreated group showed sporadic islets of calcification without coherent bone formation, and adjacent fibrous tissue had infiltrated the transplants. Native controls and corticoid-treated transplants did not exhibit bone generation or signs of fibrous tissue infiltration. This study found that immunomodulation by intramuscular methylprednisolone application protects tissue-engineered autogenous chondrocyte transplants from fibrous tissue infiltration and induces trabecular bone formation.

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Acknowledgements

The authors thank the DFG (German Research Foundation) for the grant Ha 2721-1-3.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    Andreas Haisch, Frank Wanjura & Andreas Gröger

  2. Department of Pathology, Charité, Humboldt University of Berlin, Schumannstr. 20-21, 10098 , Berlin, Germany

    Cornelia Radke

  3. Department of Pathology, University Medical Center Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 , Berlin, Germany

    Korinna Leder-Jöhrens

  4. Interdisciplinary Tissue Engineering Laboratories, Department of Rheumatology, Charité, Humboldt University of Berlin, Tucholskystr. 2, 10115, Berlin, Germany

    Michaela Endres, Svea Klaering, Alexander Loch & Michael Sittinger

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  1. Andreas Haisch
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Correspondence to Andreas Haisch.

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Haisch, A., Wanjura, F., Radke, C. et al. Immunomodulation of tissue-engineered transplants: in vivo bone generation from methylprednisolone-stimulated chondrocytes. Eur Arch Otorhinolaryngol 261, 216–224 (2004). https://doi.org/10.1007/s00405-003-0646-3

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  • DOI: https://doi.org/10.1007/s00405-003-0646-3

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