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Diversity, phylogeny and host specificity of soybean and peanut bradyrhizobia

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Abstract

A study on the diversity, phylogeny, and host specificity of soybean (Glycine max L.) and peanut (Arachis hypogaea L.) bradyrhizobia was conducted based on the 16S ribosomal RNA (rRNA) restriction fragment length polymorphisms (RFLPs), 16S rRNA sequencing, and 16S–23S rRNA intergenetic spacer (IGS) RFLP assays. Based on 16S rRNA RFLP assay, tested bradyrhizobia were divided into five genotypes, which could be further clustered into five groups by IGS RFLP assays. According to the 16S rRNA sequencing, strains of IGS-II, IV, and V were phylogenetically related to Bradyrhizobium liaoningense, Bradyrhizobium japonicum, and Bradyrhizobium elkanii, while strains of IGS-Ic and IGS-III related to Bradyrhizobium yuanmingense and Bradyrhizobium canariense, respectively. All isolates could crossly nodulate Phaseolus vulgaris, forming small white nodules. Strains of IGS-II originally isolated from peanut could efficiently nodulate Glycine soja, and two strains isolated from soybean could also nodulate peanut.

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Acknowledgements

This work was granted by Chinese High-tech Developing Program 2007AA05Z417, Chinese Microbe Resource Project 2005DKA21208-6 and Grant of State Key Laboratory of Agricultural Microbiology, HAU, China.

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  1. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Jiang Ke Yang

  2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Jun Chu Zhou

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  1. Jiang Ke Yang
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Correspondence to Jiang Ke Yang.

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Yang, J.K., Zhou, J.C. Diversity, phylogeny and host specificity of soybean and peanut bradyrhizobia. Biol Fertil Soils 44, 843–851 (2008). https://doi.org/10.1007/s00374-008-0269-3

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  • DOI: https://doi.org/10.1007/s00374-008-0269-3

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