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Effects of liming and tillage systems on microbial biomass and glycosidases in soils

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Abstract

This study was undertaken to investigate the long-term influence of lime application and tillage systems (no-till, ridge-till and chisel plow) on soil microbial biomass C (Cmic) and N (Nmic) and the activities of glycosidases (α- and β-glucosidases, α- and β-galactosidases and β-glucosaminidase) at their optimal pH values in soils at four agroecosystem sites [Southeast Research Center (SERC), Southwest Research Center (SWRC), Northwest Research Center (NWRC), and Northeast Research Center (NERC)] in Iowa, USA. Results showed that, in general, the Cmic and Nmic values were significantly (P <0.001) and positively correlated with soil pH. Each lime application and tillage system significantly (P <0.001) affected activities of the glycosidases. With the exception of α-glucosidase activity, there was no lime×tillage interaction effect. Simple correlation coefficients between the enzyme activities and soil pH values ranged from 0.51 (P <0.05) for the activity of α-glucosidase at the NWRC site (surface of the no-till) to 0.98 (P <0.001) at the SWRC site. To assess the sensitivity of the enzymes to changes in soil pH, the linear regression lines were expressed in Δactivity/ΔpH values. In general, their order of sensitivity to changes in soil pH was consistent across the study sites as follow: β-glucosidase>β-glucosaminidase>β-galactosidase>α-galactosidase>α-glucosidase. Lime application did not significantly affect the specific activities (g p -nitrophenol released kg–1 soil organic C h–1) of the enzymes. Among the glycosidases studied, β-glucosidase and β-glucosaminidase were the most sensitive to soil management practices. Therefore, the activities of these enzymes may provide reliable long-term monitoring tools as early indicators of changes in soil properties induced by liming and tillage systems.

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References

  • Acosta-Martinez V, Tabatabai MA (2000) Enzyme activities in a limed agricultural soil. Biol Fertil Soils 31:85–91

    CAS  Google Scholar 

  • Arshad MA, Schnitzer M, Angers DA, Rippmeester JA (1990) Effects of till, vs. no-till on quality of soil organic matter. Soil Biol Biochem 22:595–599

    Article  CAS  Google Scholar 

  • Baath E, Berg B, Lohm U, Lundgren B, Lundkvist H, Rosswall T, Soderstrom B, Wiren A (1980) Effects of experimental acidification and liming on soil organisms and decomposition in a Scots pine forest. Pedobiologia 20:85–100

    Google Scholar 

  • Baath E, Frostegard A, Fritze H (1992) Soil bacterial biomass, activity, phospholipid fatty acid pattern, and pH tolerance in an area polluted with alkaline dust deposition. Appl Environ Microbiol 58:4026–4031

    Google Scholar 

  • Bardgett RD, Leemans DK (1995) The short-term effects of cessation of fertiliser applications, liming, and grazing on microbial biomass and activity in a reseeded upland grassland soil. Biol Fertil Soils 19:148–154

    Google Scholar 

  • Barr AJ, Godnight JH, Sall JP, Helwig JI (1976) A user's guide to SAS. SAS Institute, Cary, N.C.

  • Bergstrom DW, Monreal CM, King DJ (1998) Sensitivity of soil enzyme activities to conservation practices. Soil Sci Soc Am J 62:1286–1295

    CAS  Google Scholar 

  • Brookes PC (1995) The use of microbial parameters in monitoring soil pollution by heavy metals. Biol Fertil Soils 19:269–279

    CAS  Google Scholar 

  • Campbell CA, LaFond GP, Leysohon AJ, Zentner RP, Janzen HH (1991) Effect of cropping practices on the initial rate of N mineralization in a thin Black Chernozem. Can J Soil Sci 71:43–45

    CAS  Google Scholar 

  • Carter MR (1986) Microbial biomass as an index for tillage-induced changes in soil biological properties. Soil Till Res 7:29–40

    Article  Google Scholar 

  • Carter MR, Rennie DA (1982) Changes in soil quality under zero tillage farming systems: Distribution of microbial biomass and mineralizable C and N potentials. Can J Soil Sci 62:587–597

    CAS  Google Scholar 

  • Christensen BT (1996) Matching measurable soil organic matter fractions with conceptual pools in simulation models of carbon turnover: revision of model structure. In: Powlson DS, Smith P, Smith JU (eds) Evaluation of soil organic matter models using existing long-term datasets. Global environmental change. (Nato ASI series, vol 38) Springer, Berlin Heidelberg New York, pp 143–160

  • Curci M, Pizzigallo MDR, Crecchio C, Mininni R (1997) Effects of conventional tillage on biochemical properties of soils. Biol Fertil Soils 25:1–6

    Article  CAS  Google Scholar 

  • Deng SP, Tabatabai MA (1996) Effect of tillage and residue management on enzyme activities in soils. II. Glycosidases. Biol Fertil Soils 22:208–213

    CAS  Google Scholar 

  • Dick RP (1992) A review: long-term effects of agricultural systems on soil biochemical and microbial parameters. Agric Ecosyst Environ 40:25–36

    CAS  Google Scholar 

  • Dick RP (1994) Soil enzyme activities as indicators of soil quality. In: Doran JW, Coleman DC, Bezdicek DF, Stewart BA (eds) Defining soil for a sustainable environment. SSSA special publication no. 35. Soil Science Society of America, Madison, Wis., pp 3–21

  • Dick RP, Rasmussen PE, Kerle EA (1988) Influence of long-term residue management on soil enzyme activities in relation to soil chemical properties of a wheat-fallow system. Biol Fertil Soils 6:159–164

    CAS  Google Scholar 

  • Doran JW, Power JF (1983) The effects of tillage on the nitrogen cycle in corn and wheat production. In: Lowrance RW, et al. (eds) Nutrient cycling in agricultural ecosystems. University of Georgia Experimental Station special publication no. 23. University of Georgia, Ga., pp 441–455

  • Doran JW, Elliott ET, Paustian K (1998) Soil microbial activity, nitrogen cycling, and long-term changes in organic C pools as related to fallow tillage management. Soil Tillage Res 49:3–18

    Article  Google Scholar 

  • Edmeades DC, Judd M, Sarathchandra SU (1981) The effect of tillage and lime on nitrogen mineralization as measured by grass growth. Plant Soil 60:177–186

    CAS  Google Scholar 

  • Eivazi F, Tabatabai MA (1988) Glucosidases and galactosidases in soils. Soil Biol Biochem 20:601–606

    CAS  Google Scholar 

  • Ekenler M, Tabatabai MA (2002) β-Glucosaminidase activity of soils: effect of cropping systems and its relationship to nitrogen mineralization. Biol Fertil Soils 36:367–376

    Article  CAS  Google Scholar 

  • Fan LT, Lee YH (1983) Kinetic studies of enzymatic hydrolysis of insoluble cellulose: derivation of a mechanistic kinetic model. Biotechnol Bioeng 15:2707–2733

    Google Scholar 

  • Frankenberger WT Jr, Dick WA (1983) Relationships between enzyme activities and microbial growth and activity indices in soil. Soil Sci Soc Am J 47:945–951

    CAS  Google Scholar 

  • Friedel JK, Munch JC, Fischer WR (1996) Soil microbial properties and the assessment of available soil organic matter in a Haplic Luvisol after several years of different cultivation and crop rotation. Soil Biol Biochem 28:479–488

    Google Scholar 

  • Horwath WR, Paul EA (1994) Microbial biomass. In: Weaver RW, Angel JS, Bottomley PS (eds) Methods of soil analysis. Part 2. (Book series no. 5) Soil Science Society of America, Madison, Wis., pp 753–773

  • Illmer P Schinner F (1991) Effects of lime and nutrient salts on the microbiological activities of forest soils. Biol Fertil Soils 11:261-266

    Google Scholar 

  • Jenkinson DS, Ladd JN (1981) Microbial biomass in soil: measurement and turnover. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol 5. Decker, New York, pp 415–417

  • Jordan D, Kremer RJ, Bergfield WA, Kim KY, Cacnio VN (1995) Evaluation of microbial methods as potential indicators of soil quality in historical agricultural fields. Biol Fertil Soils 19:297–302

    Google Scholar 

  • Kandeler E, Tscherko D, Spiegel H (1999) Long-term monitoring of microbial biomass, N mineralization and enzyme activities of Chernozem under different tillage management. Biol Fertil Soils 28:343–351

    CAS  Google Scholar 

  • Keeney DR, Nelson DW (1982) Nitrogen—inorganic forms. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. Part 2, 2nd edn. Agronomy monograph no. 9. American Society of Agronomy, Soil Science Society of America, Madison, Wis., pp 642–698

  • Klose S, Tabatabai MA (2000) Urease activity of microbial biomass in soils as affected by cropping systems. Biol Fertil Soils 31:191–199

    CAS  Google Scholar 

  • Klose S, Tabatabai MA (2002) Response of glycosidases in soils to chloroform fumigation. Biol Fertil Soils 35:262–269

    Article  CAS  Google Scholar 

  • Klose S, Moore JM, Tabatabai MA (1999) Arylsulfatse activity of microbial biomass in soils as affected by cropping systems. Biol Fertil Soils 29:46–54

    CAS  Google Scholar 

  • Kratz W, Brose A, Weigmann G (1991) The influence of lime application in damaged pine forest ecosystems in Berlin (FRG): soil chemical and biological aspects. In: Revera O (ed) Terrestrial and aquatic ecosystems: perturbation and recovery. Horwood, Chichester, pp 464–471

  • Parham JA, Deng SP (2000) Detection, quantification and characterization of β-glucosaminidase activity in soil. Soil Biol Biochem 32:1183–1190

    CAS  Google Scholar 

  • Powlson DS, Brookes P, Christensen BT (1987) Measurement of soil microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation. Soil Biol Biochem 19:59–164

    Article  Google Scholar 

  • Priha O, Smolander A (1994) Fumigation-extraction and substrate-induced respiration derived microbial biomass C, and respiration rate in limed soil of Scots pine sapling stands. Biol Fertil Soils 17:301–308

    CAS  Google Scholar 

  • Saddler JN (1986) Factors limiting the efficiency of cellulase enzymes. Microbiol Sci 3:84–87

    CAS  PubMed  Google Scholar 

  • Shah Z, Adams WA, Haven CDV (1990) Composition and activity of soil microbial population in an acidic upland soil and effects of liming. Soil Biol Biochem 22:257–263

    Article  Google Scholar 

  • Simard R, Angers DA, Lapierre C (1994) Soil organic matter quality as influenced by tillage, lime, and phosphorus. Biol Fertil Soils 18:13–18

    CAS  Google Scholar 

  • Smith JL, Paul EA (1990) The significance of soil microbial biomass estimations. In: Bollag JM, Stotzky G (eds) Soil biochemistry, vol 6. Decker, New York, pp 357–396

  • Stryer L (1988) Biochemistry, 3rd edn. Freeman, New York

  • Tabatabai MA (1994) Soil enzymes. In: Weaver RW, Angel JS, Bottomley PS (eds) Methods of soil analysis. Part 2. (Book series no. 5) Soil Science Society of America, Madison, Wis., pp 775–833

  • Tscherko D, Kandeler E (1997) Biomonitoring of soils—denitrification enzyme activity and soil microbial processes as indicators for environmental stress. In: Becker KH, Wieser P (eds) Proceedings of the 7th International Workshop on Nitrous Oxide Emissions, Cologne, 21–23 April 1997. Physikalische Chemie, Bericht 41. Wuppertal, pp 373–381

  • Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707

    CAS  Google Scholar 

  • Webb EC (1984) Enzyme nomenclature. Academic Press, New York

  • Wood CW, Torbert HA, Rogers HH, Runion GB, Prior SA (1994). Free-air CO2 enrichment effects on soil carbon and nitrogen. Agric For Meteorol 70:103–116

    Article  Google Scholar 

  • Zelles L, Scheunert I, Kreutzer K (1987) Bioactivity in limed soil of spruce forest. Biol Fertil Soils 3:211–216

    CAS  Google Scholar 

  • Zelles L, Stepper I, Zsolnay A (1990) The effect of liming on microbial activity in spruce ( Picea abies L.) forests. Biol Fertil Soils 9:78–82

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported in part by the Biotechnology By-products Consortium of Iowa and by the Iowa Limestone Producers Association.

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  1. Department of Agronomy, Iowa State University, Ames, Iowa, 50011-1010, USA

    M. Ekenler & M. A. Tabatabai

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  1. M. Ekenler
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  2. M. A. Tabatabai
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Ekenler, M., Tabatabai, M.A. Effects of liming and tillage systems on microbial biomass and glycosidases in soils. Biol Fertil Soils 39, 51–61 (2003). https://doi.org/10.1007/s00374-003-0664-8

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