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Spermine Promotes Acclimation to Osmotic Stress by Modifying Antioxidant, Abscisic Acid, and Jasmonic Acid Signals in Soybean

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Abstract

The possible involvement of spermine (Spm) in the acclimation of soybean to osmotic stress was investigated by determining the changes in photosynthetic pigments, antioxidants, and plant hormone levels in response to applied Spm. Plants were exposed to 9 % PEG-induced osmotic stress with or without 0.4 μM Spm. Osmotic stress reduced the relative water content, chlorophyll a, chlorophyll b, carotenoid, and protein contents in leaves, and these detrimental effects were alleviated by treatment with Spm. Moreover, the significant increase in the content of abscisic acid and decrease in that of jasmonic acid in plants subjected to osmotic stress was attenuated by treatment with Spm. Osmotic stress caused a significant increase in lipid peroxidation when compared to controls, and that was accompanied by a slight reduction in the level of antioxidants and reduced glutathione and in the activities of catalase, superoxide dismutase, peroxidase, and polyphenol oxidase. Spm treatment ameliorated these osmotic stress effects by reducing lipid peroxidation and increasing catalase, superoxide dismutase, peroxidase, and polyphenol oxidase activities. These results indicate that application of Spm could be exploited to alleviate a moderate level of osmotic stress through the regulation of stress-related components such as photosynthetic pigments, plant hormones, and antioxidants.

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Acknowledgments

The authors are very grateful to National Research Foundation of Korea (NRF), Ministry of Education, Science and Technology for financial support through Basic Science Research Program (2011-0022027).

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Sangyuk-Dong, Buk-gu, Daegu, 702-701, South Korea

    Ramalingam Radhakrishnan & In-Jung Lee

  2. Crop Physiology Lab, School of Applied Biosciences, Kyungpook National University, Daegu, 701-702, South Korea

    In-Jung Lee

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  1. Ramalingam Radhakrishnan
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Correspondence to In-Jung Lee.

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Radhakrishnan, R., Lee, IJ. Spermine Promotes Acclimation to Osmotic Stress by Modifying Antioxidant, Abscisic Acid, and Jasmonic Acid Signals in Soybean. J Plant Growth Regul 32, 22–30 (2013). https://doi.org/10.1007/s00344-012-9274-8

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  • DOI: https://doi.org/10.1007/s00344-012-9274-8

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