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The effect of auxin and strigolactone on ATP/ADP isopentenyltransferase expression and the regulation of apical dominance in peach

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Abstract

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We confirmed the roles of auxin, CK, and strigolactones in apical dominance in peach and established a model of plant hormonal control of apical dominance in peach.

Abstract

Auxin, cytokinin, and strigolactone play important roles in apical dominance. In this study, we analyzed the effect of auxin and strigolactone on the expression of ATP/ADP isopentenyltransferase (IPT) genes (key cytokinin biosynthesis genes) and the regulation of apical dominance in peach. After decapitation, the expression levels of PpIPT1, PpIPT3, and PpIPT5a in nodal stems sharply increased. This observation is consistent with the changes in tZ-type and iP-type cytokinin levels in nodal stems and axillary buds observed after treatment; these changes are required to promote the outgrowth of axillary buds in peach. These results suggest that ATP/ADP PpIPT genes in nodal stems are key genes for cytokinin biosynthesis, as they promote the outgrowth of axillary buds. We also found that auxin and strigolactone inhibited the outgrowth of axillary buds. After decapitation, IAA treatment inhibited the expression of ATP/ADP PpIPTs in nodal stems to impede the increase in cytokinin levels. By contrast, after GR24 (GR24 strigolactone) treatment, the expression of ATP/ADP IPT genes and cytokinin levels still increased markedly, but the rate of increase in gene expression was markedly lower than that observed after decapitation in the absence of IAA (indole-3-acetic acid) treatment. In addition, GR24 inhibited basipetal auxin transport at the nodes (by limiting the expression of PpPIN1a in nodal stems), thereby inhibiting ATP/ADP PpIPT expression in nodal stems. Therefore, strigolactone inhibits the outgrowth of axillary buds in peach only when terminal buds are present.

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Abbreviations

GR24:

(3aR*,8bS*,E)-3-(((R*)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-3,3a,4,8b-tetrahydro-2H-indeno[1,2-b]furan-2-one, a synthetic strigolactone

iP:

Isopentenyladenine

iPR:

iP riboside

IAA:

Indole-3-acetic acid

SL:

Strigolactone

PpIPT:

Peach gene adenosine phosphate isopentenyltransferase

tZ:

Trans-zeatin

tZR:

Trans-zeatin riboside

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Funding

This work was supported by the China Agriculture Research System [CARS-31-3-03].

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Authors and Affiliations

  1. Beijing Academy of Forestry and Pomology Sciences, Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, Beijing, 100093, People’s Republic of China

    MinJi Li & Qinping Wei

  2. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong, 271018, People’s Republic of China

    Yuansong Xiao & FuTian Peng

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  1. MinJi Li
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  2. Qinping Wei
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  3. Yuansong Xiao
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  4. FuTian Peng
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Correspondence to FuTian Peng.

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Communicated by Hiroyasu Ebinuma.

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Li, M., Wei, Q., Xiao, Y. et al. The effect of auxin and strigolactone on ATP/ADP isopentenyltransferase expression and the regulation of apical dominance in peach. Plant Cell Rep 37, 1693–1705 (2018). https://doi.org/10.1007/s00299-018-2343-0

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