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Wheat gene TaS3 contributes to powdery mildew susceptibility

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Abstract

Key message

Identification of TaS3 as a potential susceptibility gene encoding a protein homologous to ULP1 protease in wheat, which may regulate SUMO function facilitating powdery mildew attack.

Abstract

Some plant genes that are required for susceptibilities to certain pathogens are known as susceptibility genes or susceptibility factors, whose loss-of-function mutations can confer the plants resistances. To identify potential susceptibility genes to powdery mildew in wheat, differentially expressed genes in compatible and incompatible interactions between wheat and powdery mildew were examined by the cDNA chip assay. The genes exclusively expressed in the susceptible cultivar were interfered using biolistic transient transformation in wheat epidermal cells. The suppression of gene TaS3 (Triticum aestivum susceptibility) decreased the pathogen penetration by 19 %, and its over-expression increased the disease susceptibility. The deduced protein from TaS3 belongs to the putative ubiquitin-like protease 1 peptidase domain family. Subcellular localization studies revealed that its protein was accumulated in the nucleus. Quantitative real-time polymerase chain reaction analysis revealed that TaS3 transcript was significantly induced in the compatible host. This suggests that TaS3 is a potential susceptible gene and its function may be related to regulate SUMO functions.

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Abbreviations

qRT-PCR:

Quantitative real-time polymerase chain reaction

RACE:

Rapid amplification of cDNA ends

TaS3 :

Triticum aestivum susceptibility

ULP1:

Ubiquitin-like protease 1

SUMO:

A small ubiquitin-related modifier

hpi:

Hour post inoculation

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Acknowledgments

We would like to thank Dr. Bo Zhou, Dr. Xueping Zhou for the gift of pENTR™, pANDA-mini and pCHF3-GFP, respectively. We appreciate Dr. Hongyan Liu for the seeds of Yumai 13, Hongyou and Chiyacao, and Dr. Zhenghe Li for providing the RFP-H2B tobacco seeds. Yunqin Li supplied technical assistance in confocal laser microscopy. This work was supported by grants of the National Natural Science Foundation of China (Grant No. 30871609), the Special Fund for Agro-scientific Research in the Public Interest (201303016) and Sino-Swiss Science and Technology Cooperation (SSSTC) (Grant No. EG10-032010).

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  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Shaohui Li, Mingli Yong, Qide Deng, Zhengyi Wang & Dongwei Hu

  2. State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Rui Ji

  3. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland

    Robert Dudler

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  1. Shaohui Li
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Correspondence to Dongwei Hu.

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Communicated by J. Register.

S. Li and R. Ji contributed equally to this work.

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Li, S., Ji, R., Dudler, R. et al. Wheat gene TaS3 contributes to powdery mildew susceptibility. Plant Cell Rep 32, 1891–1901 (2013). https://doi.org/10.1007/s00299-013-1501-7

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