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Aluminum-induced cell death of barley-root border cells is correlated with peroxidase- and oxalate oxidase-mediated hydrogen peroxide production

  • Biotic and Abiotic Stress
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Abstract

The function of root border cells (RBC) during aluminum (Al) stress and the involvement of oxalate oxidase, peroxidase and H2O2 generation in Al toxicity were studied in barley roots. Our results suggest that RBC effectively protect the barley root tip from Al relative to the situation in roots cultivated in hydroponics where RBC are not sustained in the area surrounding the root tip. The removal of RBC from Al-treated roots increased root growth inhibition, Al and Evans blue uptake, inhibition of RBC production, the level of dead RBC, peroxidase and oxalate oxidase activity and the production of H2O2. Our results suggest that even though RBC actively produce active oxygen species during Al stress, their role in the protection of root tips against Al toxicity is to chelate Al in their dead cell body.

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Abbreviations

AOS:

Active oxygen species

DPI:

Diphenyleneiodonium chloride

RBC:

Root border cells

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Acknowledgements

This investigation was supported by the Science and Technology Assistance Agency under the contract No. APVT-51-001002

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, 84523, Bratislava, Slovak Republic

    L. Tamás, S. Budíková, J. Huttová, I. Mistrík, M. Šimonovičová & B. Široká

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  1. L. Tamás
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  2. S. Budíková
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  3. J. Huttová
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  4. I. Mistrík
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  5. M. Šimonovičová
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  6. B. Široká
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Corresponding author

Correspondence to L. Tamás.

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Communicated by L. Peña

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Tamás, L., Budíková, S., Huttová, J. et al. Aluminum-induced cell death of barley-root border cells is correlated with peroxidase- and oxalate oxidase-mediated hydrogen peroxide production. Plant Cell Rep 24, 189–194 (2005). https://doi.org/10.1007/s00299-005-0939-7

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  • DOI: https://doi.org/10.1007/s00299-005-0939-7

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